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Tumor-targeting living body multimodality imaging method based on gold nano-clusters

A multi-modal imaging and gold nanocluster technology, applied in echo/ultrasound imaging agents, biochemical equipment and methods, pharmaceutical formulations, etc., can solve the problems of high biological toxicity, poor stability of probes, and low signal enhancement in vivo and other issues to achieve the effect of avoiding biological toxicity and realizing timely tracking

Active Publication Date: 2014-09-10
SOUTHEAST UNIV
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  • Summary
  • Abstract
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Problems solved by technology

We also encountered many difficulties in the development of cancer-specific imaging reagents, including: poor transport of probes targeting tissue or tumors; high biological toxicity; poor stability of probes; low signal enhancement in vivo, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0028] Example 1 Imaging method based on intracellular in situ biosynthesis of gold nanoclusters

[0029] First, incubate HepG2 cells with a sterile concentration of 1-1000 μmol / L chloroauric acid or its salt solution for 16-48 hours (37 °C, 5 % CO 2 , RH 95%) to obtain gold nanoclusters with in situ biosynthesis in vivo. Then carefully remove the culture medium in the culture dish, and gently rinse the cells with sterile PBS with pH 7.2, place them under a laser focusing fluorescence microscope, and use blue light with a wavelength of 488 nm for excitation to collect the green fluorescence of the cells In the image, it can be clearly observed that the gold nanoclusters are mainly concentrated in the nucleus area by fluorescence tomography.

example 2

[0030] Example 2 Tumor targeting imaging method based on in situ growth of gold nanoclusters in living lesions

[0031]Firstly, 0.1-0.5 mL sterile chloroauric acid or its salt solution with a concentration of 1-1000 mmol / L was injected subcutaneously near the tumor of nude mice implanted with liver cancer tumor model, and after incubation for 12-36 hours, the The experimental nude mice were anesthetized with 5% isoflurane gas, and then placed on the operating platform of the small animal in vivo imager, and the images of the tumor area could be collected by selecting blue light excitation.

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Abstract

The invention discloses a method for in vivo multimodal imaging analysis of tumor targeting based on gold nanoclusters: firstly, through experiments on in vitro cells (including different types of tumor cells and normal cells), the relevant cells are mixed with a certain concentration of chloroauric acid and its Gold nanoclusters were produced by incubating in saline solution under physiological conditions, which realized real-time non-invasive high-resolution tumor cell fluorescence imaging, Raman imaging and ultrasound imaging. On this basis, local subcutaneous injection was further used to realize real-time orthotopic tumor-targeted fluorescence, Raman and ultrasound imaging on a nude mouse model with transplanted tumors. The functional nanoprobe integrates tumor targeting, fluorescence imaging, Raman imaging and ultrasound imaging, and can realize multimodal simultaneous monitoring; at the same time, this in situ in vivo imaging method of gold nanoclusters can Accurate localization and tumor-targeted imaging analysis.

Description

technical field [0001] The present invention relates to the technical field of tumor detection, and more specifically relates to a method for preparing tumor-targeting nanoprobes. Nanobiological probes such as gold nanoclusters with tumor-targeting molecular imaging are grown in situ on living body lesions. Functional nanoprobes integrate tumor targeting, fluorescence imaging, Raman imaging, and ultrasound imaging into one, and can realize multi-modal simultaneous monitoring, and this in situ in vivo imaging method can not only perform early diagnosis of tumors but also in Timely monitoring during cancer treatment. Background technique [0002] Tumors, especially malignant tumors - Cancer is the leading cause of death in humans. Despite great developments in tumor biology and oncology medicine, such as the discovery of tumor biomarkers, facile surgical procedures, and the development of radiotherapy and chemotherapy, the overall cancer survival rate has not improved signifi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N29/02G01N21/65G01N21/64A61K49/00A61K49/22C12Q1/02
CPCG01N21/65A61K49/0017A61K49/22A61K49/0065A61K49/0002G01N21/6486G01N29/00G01N21/6458G01N33/582G01N33/5005A61B5/0071A61B8/085A61B8/481A61K49/0093A61K49/222
Inventor 王雪梅王建玲李奇维姜晖
Owner SOUTHEAST UNIV
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