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Lactobacillus casei and application of lactobacillus casei to feed

A technology of Lactobacillus casei and feed, applied in the field of bio-fermentation technology and feed, can solve the problems of low number of viable fermentation bacteria, low enzyme production, slow growth of strains, etc., and achieve good anti-oxidation and antibacterial ability, fermentation activity The effect of increasing the number of bacteria and increasing daily weight gain

Active Publication Date: 2012-11-21
无锡泰仑达化机设备有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the characteristics of the strain, Streptomyces has some disadvantages in the feed fermentation process, such as slow growth of the strain, low number of fermenting viable bacteria, low enzyme production, and large differences in toxicological properties of different strains within the species, etc., which directly affect the fermentation process. The conversion rate of plant phospholipids in the process leads to low feed conversion rate and ineffective growth promotion effect

Method used

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  • Lactobacillus casei and application of lactobacillus casei to feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Weigh 5 kg of bean cake powder, 1 kg of rapeseed meal, and 0.1 kg of sunflower seeds, crush them to 20 mesh with a pulverizer, and prepare them as a matrix, add 10.9 kg of water, and add 0.061 kg of cornstarch, 0.0061 kg of ammonium sulfate, and Sodium chloride 0.037kg, stirred evenly, prepared into a solid-state fermentation medium, put into a solid-state fermentation tank, boiled at 100°C for 30min, cooled with sterile cold air for later use; take the above-mentioned Lactobacillus casei seed liquid to account for the total weight of the substrate 1% of the inoculum was inoculated into the above-mentioned solid-state fermentation medium, and the solid-state fermentation conditions were as follows: the initial pH value of the fermentation was 6.0, the product temperature during the fermentation was 25°C, and the fermentation time was 24 hours. Air-dried to obtain a feed additive. The additive is bagged and sealed, and stored in a ventilated, cool and dry place. Adopt M...

Embodiment 2

[0034] Weigh 5kg of bean cake powder and 2kg of rapeseed meal, pulverize them to 30 mesh with a pulverizer, prepare a matrix, add 9kg of water, add 0.21kg of cornstarch, 0.035kg of ammonium sulfate, and 0.049kg of sodium chloride while adding water, and stir Evenly, prepare a solid-state fermentation medium, put it into a solid-state fermentation tank, cook at 100 ° C for 30 minutes, and cool it with sterile cold air for later use; take the above-mentioned Lactobacillus casei seed liquid to account for 2% of the total weight of the substrate. Inoculated in a solid-state fermentation medium, the solid-state fermentation conditions are as follows: the initial pH value of the fermentation is 6.0, the product temperature during the fermentation process is 30°C, the fermentation time is 36h, and after the fermentation is completed, it is air-dried in an air dryer at 40°C to obtain a feed additive . The additive is bagged and sealed, and stored in a ventilated, cool and dry place. ...

Embodiment 3

[0036] Weigh 1000kg of bean cake powder, 200kg of rapeseed meal, and 30kg of sunflower seed meal, pulverize them to 40 meshes with a pulverizer, prepare a matrix, add 1797kg of water, and add 61.5kg of cornstarch, 3.69kg of ammonium sulfate, and Sodium 6.15kg, stir evenly, be mixed with solid-state fermentation medium, put into cement fermentation tank; Get above-mentioned Lactobacillus casei seed liquid to account for the inoculum amount of 3% of described substrate gross weight, inoculate in solid-state fermentation medium, solid-state fermentation The conditions are as follows: the initial pH value of the fermentation is 6.5, the product temperature during the fermentation is 35°C, the fermentation time is 24 hours, and after the fermentation is completed, it is air-dried in an air dryer at 30°C to obtain the feed additive. The additive is bagged and sealed, and stored in a ventilated, cool and dry place. Adopt MRS solid culture medium pouring plate counting method to count...

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Abstract

The invention discloses a strain of lactobacillus casei and application of the lactobacillus casei to feed. The Llactobacillus casei L-1 is obtained through screening and separation from soil, the preservation number is CCTCC (China Center for Type Culture Collection) M2012150. The strain can be fermented to produce phospholipase A2, the enzyme yield is high, the strain is applied to the feed field, oil crops containing plant phospholipid or processing byproducts of oil crops are used as fermentation substrates, ammonium sulfate, sodium chloride and corn starch are used as energy supply bodies, the strain is used as a fermentation strain, and feed additives are prepared through solid fermentation. The plant phospholipid conversion rate of the feed additives is high, good antioxidation and antibacterial capability is realized, and no toxic or side effect is caused on animals. Feeding experiments show that when the feed additives are added into a pig feed premix compound, the feed intake and the daily weight increase of pigs can be improved, the feed-weight ratio is reduced, and a growth promotion effect is realized.

Description

technical field [0001] The invention belongs to the field of biological fermentation technology and feed, in particular to a strain of Lactobacillus casei producing phosphatase A2 and its application in feed. Background technique [0002] In recent years, the application of microecological preparations in domestic and foreign feed industries has been increasing. The non-toxic, harmless, antibiotic-free and residue-free green feed additives produced by the fermentation of probiotic microorganisms can improve the digestion and absorption rate of feed and improve animal immunity. , Extended shelf life and other functions. As a major class of probiotics, Lactobacillus has a series of excellent probiotic properties, such as: acid resistance, bile acid tolerance, colonization and growth in the digestive tract and relief of intolerance, resistance to mutations, lowering serum cholesterol, It is widely used in the fields of food fermentation, animal feed and medical care for anti-t...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/245
Inventor 张梁张凌杰
Owner 无锡泰仑达化机设备有限公司
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