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Water-phase preparation method of acidity-sensitive CdTe quantum dot modified by double stabilizers

An acidity-sensitive, water-phase preparation technology, applied in the field of nano-fluorescent materials, can solve problems such as easy loss of optical labeling effect, and achieve the effects of simple and cheap experimental device, easy synthesis, and elimination of electrostatic adsorption.

Inactive Publication Date: 2013-12-11
XUZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It has been reported in the literature that the surface of the nanoprobe is modified with a hydrazone bond, and the gene fragment is linked again to carry out tumor marker research. However, the above work is to directly link the gene fragment to the hydrazone bond. When the hydrazone bond breaks, the gene fragment also follows. Detached from the nanoprobe surface, easy to lose optical labeling effect

Method used

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  • Water-phase preparation method of acidity-sensitive CdTe quantum dot modified by double stabilizers
  • Water-phase preparation method of acidity-sensitive CdTe quantum dot modified by double stabilizers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: TGA / TGH dual stabilizer modified PEG with fluorescence emission wavelength 515-618nm 4000 - Preparation of CdTe quantum dots

[0036] 1. Preparation of Sodium Telluride Hydride

[0037] 0.128g tellurium powder and 0.116g NaBH 4 Place in a 10mL reaction bottle, inject 4mL of absolute ethanol, 5mL of double-distilled water, seal it, stir at room temperature for 5h until it turns white and turbid, let stand for 1h and take the supernatant for immediate use.

[0038] Two, the preparation of mercaptoacetylhydrazine

[0039] Put 0.3mL of ethyl thioglycolate, 0.4mL of 80% hydrazine hydrate and 20mL of absolute ethanol in a reaction flask, heat to 120°C and reflux and stir for 3h, then evaporate to dryness at 90°C and add 10-15mL of water to dissolve to obtain mercaptoacetylhydrazide.

[0040] Three, the preparation of PEG aldehyde

[0041] Weigh PEG 4000 4.00g was dissolved in 40mL toluene, and the water was removed by azeotropic distillation. PEG after water ...

Embodiment 2

[0046] Example 2: TGA / TGH dual stabilizer modified PEG with fluorescence emission wavelength 510-589nm 2000 - Preparation of CdTe quantum dots

[0047] 1. Preparation of Sodium Telluride Hydride

[0048] 0.128g tellurium powder and 0.116g NaBH 4 Place in a 10mL reaction bottle, inject 4mL of absolute ethanol, 5mL of double-distilled water, seal it, stir at room temperature for 5h until it turns white and turbid, let stand for 1h and take the supernatant for immediate use.

[0049] Two, the preparation of mercaptoacetylhydrazine

[0050] Put 0.3mL of ethyl thioglycolate, 0.4mL of 80% hydrazine hydrate and 20mL of absolute ethanol in a reaction flask, heat to 120°C and reflux and stir for 3h, then evaporate to dryness at 90°C and add 10-15mL of water to dissolve to obtain mercaptoacetylhydrazide.

[0051] Three, the preparation of PEG aldehyde

[0052] Weigh PEG 2000 2.00g was dissolved in 20mL toluene, and the water was removed by azeotropic distillation. PEG after water...

Embodiment 3

[0057] Example 3: TGA / TGH dual stabilizer modified PEG with fluorescence emission wavelength 523-616nm 6000 - Preparation of CdTe quantum dots

[0058] 1. Preparation of Sodium Telluride Hydride

[0059] 0.128g tellurium powder and 0.116g NaBH 4 Place in a 10mL reaction bottle, inject 4mL of absolute ethanol, 5mL of double-distilled water, seal it, stir at room temperature for 5h until it turns white and turbid, let stand for 1h and take the supernatant for immediate use.

[0060] Two, the preparation of mercaptoacetylhydrazine

[0061] Put 0.3mL of ethyl thioglycolate, 0.4mL of 80% hydrazine hydrate and 20mL of absolute ethanol in a reaction flask, heat to 120°C and reflux and stir for 3h, then evaporate to dryness at 90°C and add 10-15mL of water to dissolve to obtain mercaptoacetylhydrazide.

[0062] Three, the preparation of PEG aldehyde

[0063] Weigh PEG 6000 6.00g was dissolved in 60mL toluene, and the water was removed by azeotropic distillation. PEG after water...

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Abstract

The invention discloses a water-phase preparation method of an acidity-sensitive CdTe quantum dot modified by double stabilizers, and belongs to the technical field of nanomaterials. Mercaptoacetic acid (or mercaptopropionic acid) and mercaptoacetyl hydrazine are used as the double stabilizers to prepared the CdTe quantum dot; then polyethylene glycol (PEG) is reacted with hydrazide bonds on the CdTe quantum dot surface through PEG aldehyde for connection; and thus the CdTe quantum dot which is modified by PEG and stabilized by double agents is prepared. The dimension of the CdTe quantum dot in aqueous solution is 2.3-4.5 nm, and the fluorescence emission wavelength is 510-620 nm. Compared with the prior art, the quantum dot can be coupled with biomacromolecules, and has acidity sensitivity and biocompatibility.

Description

technical field [0001] The invention belongs to the technical field of nano fluorescent materials, and in particular relates to a method for preparing an aqueous phase of acidity-sensitive CdTe quantum dots modified by a double stabilizer. Background technique [0002] Quantum dots are a kind of nano-fluorescent probes, which have excellent spectral properties and are more suitable for medical fluorescence imaging than organic fluorescent dyes. However, due to the small size of quantum dots, non-specific adsorption and cell phagocytosis on the surface of cells or tissues are prone to occur during the experiment, so it takes a lot of energy and time to modify and process quantum dots or tumor cells in multiple steps. Most of the preparation methods of quantum dots for fluorescent probes use colloidal chemistry, and the most commonly used modification reagents are small molecules of mercaptocarboxylic acids (such as thioglycolic acid TGA). During use, in order to reduce non-s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00C09K11/88C08G65/00
Inventor 杨冬芝孙世安汤道权杜岩
Owner XUZHOU MEDICAL COLLEGE
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