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Method for detecting single nucleotide polymorphism (SNP) of milk goat PITX2 gene and application thereof

A single nucleotide polymorphism and detection method technology, which is applied in the field of detection of single nucleotide polymorphism of dairy goat PITX2 gene, can solve the problems of lack of research and blank correlation research, and achieve simple operation, high accuracy, Easy to promote the effect of the application

Inactive Publication Date: 2014-04-09
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, research on the polymorphism of the PITX2 gene in Chinese dairy goats is lacking. The functional research of the gene locus and its genetic variation and lactation performance (such as: milk production, milk fat rate, milk fat amount, milk protein rate, milk protein amount, milk solids content, etc.) is still blank

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  • Method for detecting single nucleotide polymorphism (SNP) of milk goat PITX2 gene and application thereof
  • Method for detecting single nucleotide polymorphism (SNP) of milk goat PITX2 gene and application thereof
  • Method for detecting single nucleotide polymorphism (SNP) of milk goat PITX2 gene and application thereof

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Embodiment Construction

[0029] The present invention will be further described in detail below in conjunction with specific embodiments, which are explanations of the present invention rather than limitations.

[0030] (1) Reagents and their sources

[0031] 1. Biochemical and biological reagents:

[0032]①Taq DNA polymerase (purchased from MBI); ②Restriction enzyme RsaI (purchased from MBI); ③dNTPs (purchased from MBI); ④Marker D2000 (purchased from Beijing Tiangen Biochemical Technology Co., Ltd.); (purchased from Huamei Bioengineering Company).

[0033] 2. Common reagents:

[0034] Ordinary reagents are purchased from Huamei Bioengineering Co., Ltd., and are packaged for import: citric acid, sodium citrate, glucose, Tris, EDTA, NaCl, NaOH, KCl, Na 2 HPO 4 、KH 2 PO 4 , Tris saturated phenol, chloroform, isoamyl alcohol, absolute ethanol, sodium acetate, sodium dodecylsulfonate (SDS), ethidium bromide (EB), bromophenol blue, dimethylbenzene cyanide FF, agarose Wait.

[0035] 3. Solutions an...

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Abstract

The invention discloses a method for detecting the single nucleotide polymorphism (SNP) of a milk goat PITX2 gene. The method comprises the following steps of: amplifying a milk goat PITX2 gene by taking a milk goat genome DNA (Deoxyribose Nucleic Acid) or a DNA sequence comprising a milk goat PITX2 gene as a template and taking a PCR (Polymerase Chain Reaction) primer pair P as a primer; and performing enzyme digestion by using a restriction endonuclease RsaI, performing fragment size separation on an enzyme digestion product according to agarose gel electrophoresis, and analyzing the fragment size by using a gel imaging system to determine the SNP of the 543th locus of the milk goat PITX2 gene. The TT genotype and TC genotype of the SNP locus can be taken as effective DNA marks for remarkably increasing the milk fat content of milk goat and remarkably lowering the milk sugar content and the milk concentration simultaneously.

Description

technical field [0001] The invention belongs to the technical field of dairy goat nucleotide polymorphism, and relates to a method for detecting single nucleotide polymorphism of dairy goat PITX2 gene and an application thereof. Background technique [0002] Dairy goats play an important role in the construction of my country's dairy industry. However, compared with the basic research and practical application of dairy cows, dairy goats are extremely lagging behind in this aspect, and the overall level is very low. Important genetic resources have not been effectively tapped and utilized. In recent years, the production performance of my country's excellent dairy goat breeds, such as Guanzhong dairy goats, has declined rapidly and degraded seriously, which has directly affected the development of my country's dairy goat industry. Since the "melamine" incident, my country's goat milk products have become popular at home and abroad, and the society's demand for goat milk produ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 蓝贤勇赵海谕潘传英姜兴武陈宏雷初朝
Owner NORTHWEST A & F UNIV