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Enzyme linked immunosorbent assay (ELIAS) kit for detecting bisphenol A of water sample and detecting method

An enzyme-linked immunosorbent reagent and bisphenol technology, which is applied in the field of inspection and quarantine, can solve the problems of dependence on imports and fewer ELISA kits, and achieve the effect of reducing operation steps, low pre-treatment requirements, and low equipment requirements

Inactive Publication Date: 2012-12-19
上海出入境检验检疫局工业品与原材料检测技术中心
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  • Application Information

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Problems solved by technology

However, there are very few ELISA kits for the detection of bisphenol A that are actually used in China at present, and they mainly rely on imports.

Method used

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  • Enzyme linked immunosorbent assay (ELIAS) kit for detecting bisphenol A of water sample and detecting method
  • Enzyme linked immunosorbent assay (ELIAS) kit for detecting bisphenol A of water sample and detecting method

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Embodiment Construction

[0033] Below we will further elaborate on the enzyme-linked immunosorbent assay kit for detecting bisphenol A in water samples and its detection method of the present invention in combination with the accompanying drawings and specific examples, in order to understand more clearly the composition and composition of the present invention. Workflow, but it cannot be used to limit the protection scope of the present invention.

[0034] 1. First carry out the complete antigen synthesis of bisphenol A.

[0035] (1) Synthesis of haptens.

[0036] To organize the reaction device, put a 250mL three-neck reaction flask into a 1L glass container with an ice bath. The glass container contains ice-salt water mixture, with a digital thermometer that can be read at any time, and then place the glass container in a magnetic stirrer; Add a magnetic stirring bar into the three-necked flask, and insert a reflux condenser (anhydrous CaCl 2 Drying tube and exhaust gas absorption device), insert...

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Abstract

The invention provides an enzyme linked immunosorbent assay (ELIAS) kit for detecting bisphenol A of a water sample. The ELIAS kit comprises an ELIAS plate coated with a coating antigen, an ELIAS secondary antibody solution, a bisphenol A antibody solution, a bisphenol A standard substance solution, a substrate developing solution, a stopping solution and a concentrated scrubbing solution. According to a method for detecting the bisphenol A of the water sample with higher content of suspended solids by utilizing the ELIAS kit, the sample is preprocessed, and then the kit is used for detecting and a detection result is finally analyzed. The ELIAS kit provided by the invention is used for detecting the bisphenol A of the water sample, has the advantages of convenience in operation and high flexibility, is low in manufacturing cost, is monitored on site and is suitable for the screening of a large quantity of samples.

Description

technical field [0001] The invention relates to inspection and quarantine, in particular to an enzyme-linked immunoassay kit for detecting bisphenol A in water samples. Background technique [0002] Bisphenol A, Chinese name 2,2-bis(4-hydroxyphenyl)propane, English name 2,2-bis(4-hydroxyphenyl)propane or Bisphenol A (referred to as BPA), easily soluble in alcohol, acetone, ether , dichloromethane, benzene and dilute lye, etc., slightly soluble in carbon tetrachloride, almost insoluble in water. BPA is an important organic chemical raw material, an important derivative of phenol and acetone, mainly used in the production of polycarbonate, epoxy resin, polysulfone resin, polyphenylene ether resin, unsaturated polyester resin and other polymer materials. It can also be used in the production of plasticizers, flame retardants, antioxidants, heat stabilizers, rubber antioxidants, pesticides, coatings and other fine chemical products. [0003] BPA is almost everywhere in product...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
Inventor 吴晓红缪文彬蒋伟朱洪坤陈相
Owner 上海出入境检验检疫局工业品与原材料检测技术中心