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Folium microcotis total flavone extract and preparation method and application thereof

A technology for total flavonoids and extracts, which is applied to the total flavonoids extract of slag leaves and the fields of preparation and application thereof, can solve the problems that have not yet been found, and achieve the effect of a simple preparation method

Active Publication Date: 2014-04-23
孙冬梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, no literature reports have been found on the treatment of hyperlipidemia, coronary heart disease, cardiocerebral atherosclerosis, angina pectoris, cerebral infarction, fatty liver, and related hypertension and diabetes by the total flavonoids of Buza leaves.

Method used

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  • Folium microcotis total flavone extract and preparation method and application thereof
  • Folium microcotis total flavone extract and preparation method and application thereof
  • Folium microcotis total flavone extract and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A preparation method of total flavonoids extract of cloth dregs leaves, the steps are as follows:

[0030] (1) Rough extraction: crush the cloth residue leaves, use alcohol extraction and water precipitation extraction method, add ethanol solution with a volume fraction of 60% to reflux extraction for 3 times, filter, combine each filtrate, recover ethanol until it has no ethanol smell, and then concentrate To a suitable concentration, add an appropriate amount of water and stir evenly, let it stand still, after the precipitation is complete, pour out the supernatant, concentrate the medicinal solution until the concentration of the crude drug of cloth dregs is 0.5g / ml, obtain a crude extract, and set aside;

[0031] (2) Refining: Use non-polar macroporous adsorption resin HPD-100 for separation, first pass the above crude extract through the pretreated HPD-100 with a diameter:height ratio of 1:8 at a speed of 1.5BV / h Macroporous resin adsorption, after all pass through...

Embodiment 2

[0038] A preparation method of total flavonoids extract from leaves of cloth dregs, the steps are as follows:

[0039](1) Rough extraction: use water extraction and alcohol precipitation extraction method, first add water to decoct the cloth dregs leaves, extract 3 times, filter, combine each filtrate, concentrate to an appropriate concentration, add ethanol to adjust the concentration of the ethanol solution to 70%, let it stand , after the precipitation is complete, pour out the supernatant, recover the ethanol until it has no ethanol smell, and then concentrate until the concentration of the crude drug of cloth dregs is 3.5g / ml to obtain a crude extract, which is set aside;

[0040] (2) Refining: polyamide adsorption separation: take the crude extract, pass it through a polyamide adsorption column with a diameter:height ratio of 1:5 at a speed of 1BV / h, and elute with 10% ethanol solution to no color, and then use 1.5BV volume fraction of 95% ethanol solution to elute with ...

Embodiment 3

[0046] A preparation method of total flavonoids extract from leaves of cloth dregs, the steps are as follows:

[0047] (1) Rough extraction: pulverize the cloth dregs leaves, use alcohol percolation extraction method, add 40% ethanol to soak, carry out diafiltration, collect percolation liquid, recover ethanol, concentrate until the concentration of cloth dregs crude drug is 5g / ml, Get the crude extract and set aside.

[0048] (2) Refining: using silica gel adsorption and separation: the above liquid medicine is passed through a silica gel adsorption column with a diameter:height ratio of 1:3 at a speed of 0.5BV / h, and gradient elution is first performed with chloroform-methanol, and then methanol-water Gradient gradient elution, the specific elution process is: the volume ratio of chloroform-methanol is 4:1 to elute 1.5BV, the volume ratio is 3:1 to elute 1BV, the volume ratio is 2:1 to elute 1BV, the volume ratio is 1:1 elution 1BV, volume fraction 100% methanol elution 1BV...

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Abstract

The invention discloses a folium microcotis total flavone extract, and a preparation method and an application thereof. The extract contains 50 wt% to 95wt% of general flavone based on rutin, 3.5 wt% to 7.5 wt% of narcissoside in the total flavone extract, 0.5 wt% to 3.0 wt% of vitexin and 0.5 wt% to 3.0 wt% of isovitexin. The preparation method comprises the steps as follows: coarse extracting; taking one or more of water, methanol and ethanol as an extraction solvent for coarse extracting; refining: taking a macroporous resin or a polyamide or a silica gel as an adsorbent, ethanol or other organic solvents as an eluent for eluting, collecting the eluent, and decompressing and concentrating to obtain the folium microcotis total flavone extract. The folium microcotis total flavone extract provided by the invention contains 50 wt% to 95 wt% of the general flavone based on the rutin, and has the obvious effect of adjusting blood fat. The preparation method is simple and effective, thereby being suitable for industrial mass production and capable of preparing medicines for adjusting the blood fat.

Description

technical field [0001] The invention relates to an extract of total flavonoids from Buza leaves, a preparation method and application thereof. Background technique [0002] Hyperlipidemia is a common clinical disease, which has the characteristics of slow progress and many complications. With the improvement of people's living standards and changes in diet structure and lifestyle, the prevalence of hyperlipidemia in my country has a tendency to increase year by year. Hyperlipidemia is the main risk factor leading to atherosclerosis. Arteriosclerosis in the heart and brain can lead to coronary heart disease, angina pectoris, myocardial infarction, and cerebrovascular accidents; capillary sclerosis in the kidneys can cause renal failure. Thereby leading to obstinate hypertension and uremia; hyperlipidemia can also cause fatty liver, and severe cases can lead to liver cirrhosis. According to statistics, among adults over the age of 30 in my country, the proportion of hyperlip...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P9/10A61P1/16A61P9/12A61P3/10A61P3/06A61K127/00
Inventor 孙冬梅陈玉兴毕晓黎张建军曾晓会李智勇
Owner 孙冬梅
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