Bovine-derived anti-staphylococcus aureus single-chain antibody, preparation method and application thereof

A single-chain antibody and Staphylococcus technology, applied in the field of genetic engineering, can solve problems such as difficult to cure and easy to produce resistance to antibiotics, and achieve good application prospects
CN102863528AInactive Publication Date: 2013-01-09SHANGHAI JIAO TONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI JIAO TONG UNIV
Publication Date
2013-01-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a bovine-derived anti-staphylococcus aureus single-chain antibody. The antibody at least comprises a light chain variable region of an amino acid sequence which is represented in SEQ ID No.1, a heavy chain variable region of an amino acid sequence which is represented in SEQ ID No.2 and an intermediate connecting peptide which is located between the light chain variable region and the heavy chain variable region. The single-chain antibody can be specifically bound with staphylococcus aureus, has certain external neutralization activity and has good application prospects when used in related reagents or drugs for diagnosing, preventing and treating staphylococcus aureus bovine mastitis.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering, and in particular relates to a genetically engineered single-chain antibody against Staphylococcus aureus dairy cow mastitis, an expression vector and host cell expressing the single-chain antibody, a preparation method of the single-chain antibody and its use. The single-chain antibody can specifically bind to Staphylococcus aureus and has a certain neutralizing activity in vitro. Background technique

[0002] A single-chain antibody is a genetically engineered antibody that uses recombinant technology to convert the light chain variable region V of the antibody into a single-chain antibody. L and heavy chain variable region V H It is formed by connecting the end to the end of a short peptide linker, which is the smallest functional fragment that retains the complete antigen binding site. There are three forms of single-chain antibody: 1) direct expression in the cytoplasm; 2) fusion with ot...

Claims

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