Microbial inoculum for rehabilitating nitrotoluene polluted soil and application method thereof

A technology of nitrotoluene and polluted soil, which is applied in the field of microorganisms, achieves good degradation effect, simple preparation method, and stable operation effect

Inactive Publication Date: 2013-01-09
马小杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it cannot fully meet the actual needs. Therefore, it is imminent to develop a method for repairing nitrotoluene-contaminated soil with low construction investment, low operating cost and good treatment efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A bacterium agent for repairing nitrotoluene-contaminated soil, the active ingredient of the bacterium agent includes the following raw materials in weight percentage:

[0030] Nitrosogenic bacteria 30%, Bacillus subtilis 15%, Alcaligenes faecalis 10%, Pseudomonas aeruginosa 10%, Saccharomyces cerevisiae 10%, Rhodococcus 10%, Bacillus megaterium 8%, Aspergillus niger 7%.

[0031] Among the above bacteria agents:

[0032] The nitrosogenic bacteria are preferably Nitrosomonas europaea CCTCC No: M 2010002 (CN101955885 public use);

[0033] The Bacillus subtilis is preferably Bacillus subtilis (Bacillus subtilis) CGMCC No: 0954;

[0034] The Alcaligenes faecalis is preferably Alcaligenes faecalis ATCC 31555;

[0035] The Pseudomonas aeruginosa is preferably Pseudomonas aeruginosa ATCC 15442;

[0036]The Saccharomyces cerevisiae is preferably Saccharomyces cerevisiae CCTCC No: M 208110;

[0037] The Rhodococcus is preferably Rhodococcus (Rhodococcus rhodochrous) ATCC1590...

Embodiment 2

[0052] Nitrotoluene Contaminated Soil Remediation Experiment

[0053] Mix 200g of trinitrotoluene TNT + 200g of dinitrotoluene DNT + 100g of nitrotoluene with a total mass of 500g in 1 cubic meter of non-polluted soil, stir and dry, pass through a 20-mesh sieve for later use. First above-mentioned polluted soil is spread the thin layer of 30 centimeters of thickness, the inoculant prepared by embodiment 1 is evenly sprinkled on the surface layer of nitrotoluene polluted soil prepared in this experiment, wherein the weight ratio of inoculum and soil is 1: 2000, then Spray water, keep soil water content to be 30%, after 48 hours, detect the content of nitrotoluene, trinitrotoluene TNT is 0g / cubic meter, dinitrotoluene is 2.1g / cubic meter and the content of nitrotoluene is 0.5g / m3, indicating that the bacterial agent has basically completely degraded the nitrobenzene substances in the soil.

Embodiment 3

[0055] The bacterial agent prepared by the embodiment of the present invention 1 is used to process the polluted soil near a certain explosive factory, and the content of trinitrotoluene in the soil is 320g / cubic meter, dinitrotoluene is 72g / cubic meter and nitrotoluene 183g / cubic meter. Cubic meter; first the soil is loosened and leveled, sprayed with water, and the moisture content of the soil is maintained at 30%, then the inoculum is spread according to the addition of 500g of inoculum per cubic meter of soil, and after 2 days, the content of nitrobenzene is detected, wherein, The content of trinitrotoluene was 0 g / m3, that of dinitrotoluene was 1.3 g / m3, and that of nitrotoluene was 0.6 g / m3.

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Abstract

The invention relates to a microbial inoculum for rehabilitating nitrotoluene polluted soil and an application method thereof. The microbial inoculum is prepared from the following raw materials in percentage by weight: 30% of nitrococcus, 15% of Bacillus subtilis, 10% of Bacillus foecalis alkaligenes, 10% of Pseudomonas aeruginosa, 10% of Saccharomyces cerevisiae, 10% of Rhodococcus, 8% of Bacillus megaterium and 7% of Aspergillus niger. According to the microbial inoculum provided by the invention, the strains are matched with each other reasonably, and realize symbiotic coordination without mutual antagonism; and the microbial inoculum has a favorable degradation effect on nondegradable nitrotoluene compounds.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a bacterial agent for repairing nitrotoluene-contaminated soil and an application method thereof. Background technique [0002] TNT (2, 4, 6 trinitrotoluene) is currently the most produced explosive. In recent years, the manufacture, processing and packaging of TNT in ammunition workshops have caused highly concentrated pollution of soil, which will eventually lead to groundwater pollution. Long-term exposure to nitrotoluene can increase the chances of suffering from anemia and abnormal liver function. Blood and liver effects, enlarged spleens, and other adverse effects on the immune system were also found in animals injected or inhaled. There is also evidence that TNT has adverse effects on male reproductive function, and TNT is also listed as a possible carcinogen. At present, the treatment of such pollutants is difficult. [0003] The mode of p-nitrotoluene pol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/14B09C1/10C12R1/125C12R1/05C12R1/385C12R1/865C12R1/11C12R1/685C12R1/01
Inventor 马小杰闫家怡
Owner 马小杰
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