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Aqueous dispersible granules of trichoderma asperellum chlamydospores biopesticide and preparation of aqueous dispersible granules

A technology of spore organisms and chlamydospores, which is applied in the fields of biopesticides and biochemistry, and can solve problems such as flying dust

Active Publication Date: 2013-01-16
成都特普生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common Trichoderma conidia preparations are mainly wettable powders, which have the disadvantage of flying dust during use

Method used

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  • Aqueous dispersible granules of trichoderma asperellum chlamydospores biopesticide and preparation of aqueous dispersible granules
  • Aqueous dispersible granules of trichoderma asperellum chlamydospores biopesticide and preparation of aqueous dispersible granules
  • Aqueous dispersible granules of trichoderma asperellum chlamydospores biopesticide and preparation of aqueous dispersible granules

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Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0015] Trichoderma spores were cultured on PDA medium. Trichoderma species are Trichoderma viride, Trichoderma haziamum, Trichoderma konigii and Trichoderma auxeoviride.

[0016] Cultivate the Trichoderma strains on the culture medium: 200g of potatoes, 20g of glucose, 18-20g of agar, 1000mL of water, natural pH, sterilized at 121°C for 20min, inoculated, and cultivated at 28°C for 6 days to produce a large amount of green conidia.

[0017] Liquid fermentation culture: use soluble starch, calcium carbonate, wheat bran, ammonium sulfate and potassium dihydrogen phosphate according to the preparation ratio in Table 1, add 4.0mL of soybean oil, natural pH, 1000mL of water to make a liquid medium, and sterilize at 121°C 20min. Use an inoculation loop to transfer the conidia of Trichoderma to a test tube containing sterile water, stir to disperse the conidia from the mycelium, filter the mycelium with sterile gauze, and adjust the spore concentration to 107cfu / mL, Inoculate into...

Embodiment approach 2

[0028] Embodiment two: the preparation of water dispersible granule

[0029] Trichoderma fermented liquid (containing chlamydospore quantity 3.27 * 10 7 cfu / mL) and white carbon black at a ratio of 10:1 to make a mother liquor, dried at 37°C to make a mother powder, and added living microorganism protection agent, disintegrant and adhesive to the mother powder according to the combination ratio in Table 4 The agent is mixed evenly, and then the water-dispersible granule is obtained by extrusion granulation.

[0030] Table 4 Water Dispersible Granule Composition Optimization Result Table

[0031]

[0032] Although the active ingredient of the preparation is dormant chlamydospores, which have strong resistance to environmental conditions, their survival rate will still be affected by ultraviolet rays and temperature in the environment. A binder needs to be added to the processed water-dispersible granules to increase the granulation rate. The role of the binder is to increa...

Embodiment approach 3

[0035] The isolated and purified Trichoderma harzianum spores were cultured on PDA medium at 28° C. for 6 days to produce a large number of green conidia.

[0036]Combine 20g of soluble starch, 1.5g of calcium carbonate, 5.5g of wheat bran, 3.0g of ammonium sulfate and 2.0g of potassium dihydrogen phosphate, add 4.0mL of soybean oil, natural pH, 1000mL of water to make a liquid medium, 121°C Sterilize for 20 minutes. Use an inoculation loop to transfer the conidia of Trichoderma to a test tube containing sterile water, stir to disperse the conidia from the mycelium, filter the mycelium with sterile gauze, and adjust the concentration of the spores to 10 7 cfu / mL, inoculated into liquid fermentation medium according to 1% inoculum size, and cultured at 28°C for 6 days.

[0037] The obtained Trichoderma chlamydospore-containing 8.5×10 7 cfu / mL fermented liquid and 10% white carbon black are mixed and adsorbed, dried at 37°C to make mother powder, and 0.8% carboxymethyl cellulo...

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Abstract

The invention relates to aqueous dispersible granules of viable microbe biopesticide taking trichoderma asperellum chlamydospores as a main component, and a preparation method of the aqueous dispersible granules of viable microbe biopesticide, belonging to the technical field of biopesticide and biochemistry. The preparation method comprises the following steps of: cultivating high-concentration trichoderma asperellum chlamydospores by using a culture medium with efficient composites in a liquid fermentation manner; mixing trichoderma asperellum chlamydospores serving as an active component with a microbial thallus protective agent, a disintegration agent and an adhesive; and pelletizing and drying to finally obtain the aqueous dispersible granules of biopesticide. The aqueous dispersible granules of biopesticide has high content of trichoderma asperellum chlamydospores and strong storage property and ultraviolent resistances, and can disintegrate and disperse quickly after being dissolving in water; the dust flying during the use process is avoided; and the aqueous dispersible granules of biopesticide have the advantages of wettable powder and aqueous suspending agents, so the stability of the microbial pesticide preparations in prevention and control of plant diseases is improved greatly.

Description

technical field [0001] The invention belongs to the technical fields of biopesticide and biochemistry. Background technique [0002] With the requirements of economic development and sustainable development of the environment, finding new agricultural disease control measures that meet the sustainable development of agriculture has become a hot spot in current agricultural production research. Due to the advantages of no pollution and no residue, biological control has attracted people's attention. At present, the research and development of biocontrol strains and active antibacterial ingredients have become the focus of competition for new biopesticides. [0003] Trichoderma is an important class of biocontrol fungi. Trichoderma preparations currently on the market are all conidia preparations and mycelial preparations, mostly fermented from solids, such as Trichodex from Makhteshim Agan Company in Israel, which can be used to control Botrytis. my country also has simila...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N25/14A01P3/00
Inventor 黄永
Owner 成都特普生物科技股份有限公司
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