Method for preparing oil-removing microbial inoculum and method for treating oil field sewage by using oil-removing microbial inoculum

A technology of oil field sewage and oil bacterial agent, which is applied in the field of preparation of microbial compound bacterial agent, can solve the problems of long start-up period of biochemical treatment system, inability to adapt to oil field sewage environment, and blocked activity of microbial metabolic enzymes, etc., to achieve stable and efficient treatment , Good degreasing effect and short start-up period

Active Publication Date: 2015-03-04
CHINA PETROCHEM & CHEM JIANGSU OILFIELD BRANCH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In a high-salinity environment, the activity of microbial metabolic enzymes is hindered, and the growth rate of microorganisms is slow or does not reproduce. General oil-removing microorganisms often cannot adapt to the sewage environment of oilfields. Some biochemical treatment systems have a long start-up period for film formation, poor degreasing effect or Disadvantages such as the failure of the system to start the film

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of preparation method of degreasing bacteria agent, comprises the following steps successively:

[0036] step 1)

[0037] Oily sewage and sludge were taken from multiple oily sewage pools in Jiangsu Oilfield; bacteria in oily sewage and oily sludge in oilfields were enriched and cultured in LB medium, neutral environment, and 25-30°C, and separated after many times , purification and screening to obtain 3 strains of degreasing bacteria with high degreasing efficiency, and the 3 strains of degreasing bacteria are respectively

[0038] OFB-1: Bacillus cereus (Latin literary name Bacillus cereus ), deposited in the General Microorganism Collection Center (CGMCC) of China Committee for the Collection of Microorganisms, date of deposit: August 15, 2011, and deposit number: CGMCC NO. 5135;

[0039] OFB-2: Bacillus cereus (Latin literary name Bacillus cereus ), deposited in the General Microorganism Collection Center (CGMCC) of the China Committee for the Collection ...

Embodiment 2

[0057] Yet another preparation method of an oil-removing bacteria agent, the difference from Example 1 is that in step 2), the bacteria liquid of three strains of oil-removing bacteria is mixed by volume ratio OFB-1 strain: OFB-2 strain: OFB-3 The strains were mixed at 1: 0.9: 1.1, and the viable counts of OFB-1 strain, OFB-2 strain and OFB-3 strain were 10 9 ~10 11 pieces / ml. When the mixed bacterial liquid is fermented and scaled up in industrial culture medium; the fermentation process conditions are: fermentation temperature: 28-32°C; inoculum size: 4% v / v; stirring speed: 100 rpm; fermentation time: 30h; Air is passed into the medium, and the gas-liquid volume ratio of the air per minute is 12; the industrial medium is composed of the following components:

[0058] Rapeseed cake 0.8% w / v;

[0059] Wheat bran 1% w / v;

[0060] MgSO 4 ·7H 2 O 0.05%% w / v;

[0061] NaH 2 PO 4 0.5%% w / v;

[0062] CaCO 3 0.4%% w / v;

[0063] The balance is water. ...

Embodiment 3

[0066] Yet another method for preparing an oil-removing bacteria agent, which differs from Examples 1 and 2 in that in step 2), the bacterial liquid of three strains of oil-removing bacteria is mixed by volume ratio OFB-1 strain: OFB-2 strain: OFB -3 strains are mixed at 1: 1.1: 0.9, and the number of live bacteria of OFB-1 strain, OFB-2 strain and OFB-3 strain is 10 respectively. 9 ~10 11 a / ml;

[0067] In step 3), when the mixed bacterial liquid is fermented and scaled up in industrial medium; the fermentation process conditions are: fermentation temperature: 28-32°C; inoculum size: 6% v / v; stirring speed: 200 rpm; fermentation time: 60 h; during the fermentation process, air is introduced into the medium, and the gas-liquid volume ratio of the air per minute is 1.5; the industrial medium is composed of the following components:

[0068] Rapeseed cake 1.2% w / v;

[0069] Wheat Bran 3% w / v;

[0070] MgSO 4 ·7H 2 O 0.15% w / v;

[0071] NaH 2 PO 4 0.7% w / v;

[0...

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PUM

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Abstract

The invention discloses a method for preparing an oil-removing microbial inoculum and a method for treating oil field sewage by using the oil-removing microbial inoculum. The method for preparing the oil-removing microbial inoculum comprises the following steps of: enriching bacteria in oily sewage and oily soil of oil fields, separating, purifying and screening to obtain oil-removing bacteria which are suitable for the oily sewage of the oil fields and are high in oil removal efficiency; combining and culturing to obtain a high-efficiency compound oil-removing microbial inoculum; and processing compound microorganisms into powder with relatively high activation rate. The oil-removing microbial inoculum is applied to high-salinity oily sewage of the oil fields. The method for treating the oil field sewage by using the oil-removing microbial inoculum comprises the following steps of: performing multiplication culture on the microbial inoculum, forming a biofilm in a biochemical pool, acclimating and culturing oily wastewater, and finally treating oily sewage. The method is high in biological activity, has a good oil removal effect, and fulfills the aim of high-efficiency oil removal, and the biofilm has high impact resistance; and the method for preparing the microbial inoculum has the characteristics of simple operation and low cost, and is suitable for large-scale production and application.

Description

technical field [0001] The invention relates to a preparation method and application of a microbial composite bacterial agent, in particular to a bacterial agent for treating oily sewage in oilfields and its application in treating oilfield sewage. Background technique [0002] Oily sewage in oil fields mainly contains various components such as petroleum, suspended solids, colloids, and chemicals. including emulsified oil and dissolved oil) and degradation of organic matter to water and CO 2 , Eliminate sulfide, effectively improve the stability of effluent water quality. The biochemical treatment method can degrade and transform the polluting substances of sewage, and finally achieve harmless treatment, so that water resources can be recycled; at the same time, it can improve the service life of equipment and filtering devices; this method consumes a lot of reducing substances, crude oil and other organic substances , reducing the organic nutrients that other microorgani...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/20C02F3/34
Inventor 徐卫华薜芸杨海滨罗江涛杨帆王彪庄建全
Owner CHINA PETROCHEM & CHEM JIANGSU OILFIELD BRANCH
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