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Anoxybacillussp and application thereof

A facultative anaerobic and bacillus technology, applied in the field of petroleum exploration engineering strains, thermophilic and salt-tolerant facultative anaerobic bacillus, can solve the problems of high freezing point, difficult flow, high mining, etc., to achieve high freezing point in Liaohe Oil recovery, surface tension reduction effect

Active Publication Date: 2013-02-06
北京世纪金道石油技术开发有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Heavy oil, heavy oil, and high pouring oil are rich in waxes, colloids, and asphaltenes, and have the characteristics of high freezing point and difficult flow, so they are difficult to mine and costly to mine

Method used

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  • Anoxybacillussp and application thereof
  • Anoxybacillussp and application thereof
  • Anoxybacillussp and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Screening of thermophilic and halo-tolerant facultative anaerobic bacillus (Anoxybacillus sp.) JD-174CGMCC NO.6570 strain

[0039] Take the oil-water mixed sample of an oil well in a certain block of Liaohe Oilfield, shake and mix well, and start the first round of cultivation: take 10ml with a pipette gun, and aseptically inoculate it into 100ml inorganic salt degradation basal medium (medium composition: liquid paraffin 2% 、NaNO 3 4%, MgSO 4 ·7H 2 O 0.05%, KH 2 PO 4 1%, K 2 HPO 4 2.0%, NaCl 1%, FeSO 4 ·7H 2 O 0.01%, CaCl 2 0.01%, yeast powder 0.1%, the balance is water, pH = 7.2; 121 ° C for 30 min), add 5 g of crude oil, so that the concentration of crude oil in the basic medium for inorganic salt degradation is 5% g / ml, at 60 ° C The shaker rotates at 180 rpm, and cultures with reciprocating shaking for 7 days. Start the second round of cultivation: take 10ml of the culture solution after 7 days, insert 100ml of new inorganic salt basal mediu...

experiment example 1

[0044] Experimental example 1 Morphological characteristics, physiological and biochemical characteristics, high temperature resistance and salt tolerance of the thermophilic and halotolerant facultative anaerobic bacillus (Anoxybacillus sp.) JD-174CGMCC NO.6570

[0045] 1. Test strains

[0046] The thermophilic and halotolerant facultative anaerobic bacillus (Anoxybacillus sp.) JD-174 CGMCC NO.6570 strain isolated in Example 1 of the present invention.

[0047] 2. Experimental method

[0048] Refer to the experimental method of "Bergey's Manual of Systematic Bacteriology" to detect its Gram staining, cell size and shape, growth temperature, growth pH range, and NaCl tolerance. Perform methyl red, pyocyanin, catalase, starch hydrolysis, cellulose hydrolysis, oxidase, V-P, H 2 S, MR, phenylalanine deamination, urease, glucose acid production, lysozyme resistance, indole degradation, gelatin liquefaction and casein hydrolysis experiments.

[0049] 3. Test results

[0050] Mo...

experiment example 2

[0054] Experimental Example 2 Degradation performance test of crude oil by the bacterial strain of thermophilic and halo-tolerant facultative anaerobic bacillus (Anoxybacillus sp.) JD-174 CGMCC NO.6570 under aerobic and anaerobic conditions

[0055] 1. Test strains

[0056] The thermophilic and halotolerant facultative anaerobic bacillus (Anoxybacillus sp.) JD-174 isolated in Example 1 of the present invention has a strain preservation number of CGMCC NO.6570.

[0057] 2. Experimental method

[0058] The tested strains were activated on LB plates, and a single colony was picked and inserted into 50ml LB liquid medium for overnight culture at 65°C until the logarithmic growth phase. Inoculate the cultivated bacterium liquid with 10% inoculum to the Erlenmeyer flask that 100ml is equipped with the inorganic salt degradation basal medium (composition is identical with embodiment 1) with crude oil (5%) as carbon source. The aerobic condition is to culture the shake flask at 65°C i...

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Abstract

The invention discloses anoxybacillussp, a fungicide of the anoxybacillussp and application. The anoxybacillussp (Anoxybacillussp) JD-174 is separated from an oil sample from a high-temperature oil well, and is stored with a number of CGMCC No.6570. The invention also discloses a microbial agent comprising the anoxybacillussp and a nutrient culture medium. The invention further discloses the application of the anoxybacillussp and the microbial agent in petroleum extraction. The anoxybacillussp or the fungicide the anoxybacillussp can degrade and emulsify raw petroleum or diesel, and can effectively reduce viscosity and a freezing point of raw petroleum, and improve liquidity of the raw petroleum. By being used as a microbial oil displacement agent, the anoxybacillussp or the fungicide of the anoxybacillussp can obviously improve a recovery ratio of the petroleum.

Description

technical field [0001] The invention relates to a bacterial strain used in the field of petroleum exploitation engineering, in particular to a thermophilic, salt-tolerant and facultative anaerobic bacillus strain for improving the recovery rate of petroleum heavy oil. The invention further relates to the application of the bacteria in crude oil exploitation, and belongs to the technical field of microbial oil flooding. Background technique [0002] Petroleum is the blood of industry, the nerve artery of modern civilization, and plays an extremely important role in the development of human society. At the same time, oil is a non-renewable resource. With the increasing shortage of oil resources and the continuous increase of exploration costs, the efficiency of secondary water injection oil recovery technology has become low, which can no longer meet people's increasing demand for oil resources. In order to effectively utilize oil resources, countries all over the world are t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K8/582C12R1/01
Inventor 王小通姜利滨夏文杰李东安
Owner 北京世纪金道石油技术开发有限公司
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