Method for separating trans-resveratrol from giant knotweed

A technology of resveratrol and Polygonum cuspidatum, applied in the field of compound trans-resveratrol, can solve the problems that have not been reported on the separation of active components of molecularly imprinted hollow fiber membranes, and achieve the effect of separation of selective permeability

Inactive Publication Date: 2013-02-13
SOUTHERN MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chromatographic performance of theophylline molecularly imprinted membranes, Membrane Science and Technology, 2010, 30(5), 37...

Method used

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  • Method for separating trans-resveratrol from giant knotweed
  • Method for separating trans-resveratrol from giant knotweed
  • Method for separating trans-resveratrol from giant knotweed

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0030] Example 1 (Preparation of trans-resveratrol molecularly imprinted polymer membrane)

[0031] (1) Dissolve 0.038g trans-resveratrol and 0.071g acrylamide in 1ml acetonitrile, overnight at 4°C, then add 1g ethylene glycol dimethacrylate and 0.01g azobisisobutyronitrile , feed nitrogen to deoxygenate to obtain monomer solution;

[0032] (2) Soak the polyvinylidene fluoride (PVDF) film in the monomer solution for 1 hour, then take it out and place it between two glass slides, and place it in a vacuum drying oven at a vacuum degree of -0.09 to -0.005 Under the conditions of MPa and temperature of 52°C, the polymerization reaction was carried out for 48 hours to obtain the precursor of the resveratrol molecularly imprinted polymer membrane; the polyvinylidene fluoride membrane has the following performance parameters: the membrane pore diameter is 0.2-0.45 μm, and 1000-1400μm, wall thickness 200-300μm, porosity 80-90%, pure water flux 200-300L / m 2 .h;

[0033] (3) Wash the...

example 2

[0035] Example 2 (separation of trans-resveratrol)

[0036] 1. Extraction of trans-resveratrol

[0037] (1) Take 1g of Polygonum cuspidatum, add 50ml of 95% ethanol to reflux and extract for 2 hours, filter, concentrate the filtrate under reduced pressure to extract, disperse in water, apply AB-8 macroporous adsorption resin, and elute with water and 30% ethanol-water in sequence , collect 30% ethanol-water eluate, concentrate under reduced pressure, and dry to obtain alcohol extract of Polygonum cuspidatum;

[0038] (2) Dissolve the alcoholic extract of Polygonum cuspidatum in methanol, so that the concentration of Polygonum cuspidatum extract in the solution is 50 μg / ml to obtain a sample solution;

[0039] (3) Pump the sample solution and methanol into figure 1 The membrane separator shown, and makes the sample solution flow in the membrane tube at a speed of 0.5ml per minute, and the extractant flows in reverse at a speed of 0.5ml per minute outside the membrane tube; wh...

example 3

[0055] Example 3 (separation of trans-resveratrol)

[0056] (1) Take 1g of Polygonum cuspidatum and add 40ml of 80% ethanol to reflux to extract for 2.5h, filter, concentrate the filtrate under reduced pressure to extract, disperse in water, and wash with water and 30% ethanol-water successively on AB-8 macroporous adsorption resin , collect 30% ethanol-water eluate, concentrate under reduced pressure, and dry to obtain alcohol extract of Polygonum cuspidatum;

[0057] (2) Dissolve the alcoholic extract of Polygonum cuspidatum in water so that the concentration of Polygonum cuspidatum extract in the solution is 20 μg / ml to obtain a sample solution;

[0058] (3) Pump the sample solution and ethanol into figure 2 The membrane separator shown, and makes the sample solution flow in the membrane tube at a speed of 0.8ml per minute, and the extractant flows in reverse at a speed of 0.8ml per minute outside the membrane tube; when the inner and outer phases of the membrane tube are...

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Abstract

The invention relates to a method for separating trans-resveratrol from giant knotweed. The method comprises the following steps of: (1) crushing the giant knotweed, adding methanol or 80 to 95 volume percent ethanol in an amount which is 20 to 50 times the massic volume of the giant knotweed, extracting, purifying by using macroporous resin, concentrating under reduced pressure, and drying to obtain an alcohol extract of the giant knotweed; (2) dissolving the alcohol extract of the giant knotweed into water, methanol or acetonitrile to ensure that the concentration of the alcohol extract of the giant knotweed in the obtained solution is 10 to 1,000mu g/ml to obtain a sample solution; and (3) pumping the sample solution and an extracting agent into a membrane separator, ensuring that the sample solution flows at the rate of 0.3 to 1.0ml/min in a membrane tube and the extracting agent contraflows at the rate of 0.3 to 1.0ml/min outside the membrane tube, adding a pH regulator into the extracting agent outside the membrane tube (the volume ratio of the pH regulator to the extracting agent is 0.1:99.9-1:99) after two phases inside and outside the membrane tube are balanced, collecting an extracting solution which flows outside the membrane tube, and concentrating under reduced pressure to obtain the trans-resveratrol. The method is high in selectivity and separation efficiency.

Description

technical field [0001] The invention relates to a compound trans-resveratrol, in particular to a method for separating trans-resveratrol from traditional Chinese medicine Polygonum cuspidatum. Background technique [0002] Polygonum cuspidatum is a traditional Chinese medicine, which is the dried rhizome and root of perennial herb in the family Polygonaceae. It mainly contains anthraquinones and stilbenes, among which trans-resveratrol, a stilbene component, has important physiological activities. Resveratrol is a natural stilbene polyphenol substance in plants, and it is a phytoalexin produced by plants under the influence of harsh external environments such as fungal infection and ultraviolet light irradiation. It has obvious antibacterial, anti-inflammatory, anti-cancer, anti-thrombosis, anti-hyperlipidemia effects, especially for cardiovascular diseases. It is one of the promising anticancer active substances. As a natural active substance, trans-resveratrol has attra...

Claims

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Application Information

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IPC IPC(8): C07C39/21C07C37/70C08F220/06C08F220/56C08F226/06C08F222/14C08J9/26B01D71/40B01D71/34B01D71/68B01D67/00B01D69/12B01D61/00
Inventor 向海艳谢扬罗奇志戴开金
Owner SOUTHERN MEDICAL UNIVERSITY
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