Method for extracting buckwheat flavone from buckwheat bran
A technology of buckwheat bran and buckwheat flavonoids, which can be used in pharmaceutical formulas, non-central analgesics, medical preparations containing active ingredients, etc., and can solve the problems of low yield and content of total flavonoids, large energy consumption, and solvent residues , to achieve the effect of simple and easy operation, energy saving and fast completion time
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Embodiment 1
[0033] The method for extracting buckwheat flavonoids from buckwheat bran comprises the following steps:
[0034] (1) In this example, the sweet buckwheat bran in northern Shaanxi is used as the raw material, and the crushing method is as follows: take the sweet buckwheat bran, dry it at 60°C, crush it, pass it through a 60-mesh sieve, and set aside;
[0035] Enzymolysis under ultrasonic conditions: take the pretreated buckwheat bran, add 45 times the amount of water to make a buckwheat bran suspension, adjust the pH value to 4.6, add enzyme to buckwheat bran according to the amount of 0.54mg / ml Add the extraction compound enzyme (purchased from Ningxia Hersbit Biotechnology Co., Ltd., including cellulase, β-glucanase, xylanase, pectinase and acid protease) to the suspension, mix well, and The enzymolysis was carried out for 71 minutes at a temperature of 40° C. and a power of 200 W to obtain an enzymolysis solution.
[0036] (2) Centrifugal separation: centrifuge the enzymat...
Embodiment 2
[0052] (1) In this example, the sweet buckwheat bran in northern Shaanxi is used as raw material, and the pulverization method is as follows: take the sweet buckwheat bran, dry it at 60°C, crush it, pass it through a 60-mesh sieve, and set it aside; the specific steps of extracting buckwheat bran flavonoids are as follows:
[0053] Enzymolysis under ultrasonic conditions: Take crushed buckwheat bran, add 50 times the amount of water to make buckwheat bran suspension, adjust the pH value to 4.5, and add enzyme amount of 0.50mg / ml to buckwheat bran to suspend Add cellulase (enzyme activity ≥ 400,000 u / g), pectinase (enzyme activity ≥ 500,000 u / g) and acid protease (enzyme activity ≥ 100,000 u / g) into the turbid liquid and mix well, three of them The mass ratio of the enzymes was 1:1:1; the enzymolysis was carried out for 80 min under the conditions of an ultrasonic temperature of 40°C and a power of 200W to obtain an enzymatic hydrolysis solution.
[0054] (2) Centrifugal separa...
Embodiment 3
[0060] (1) In this example, the sweet buckwheat bran in northern Shaanxi is used as raw material, and the pulverization method is as follows: take the sweet buckwheat bran, dry it at 60°C, crush it, pass it through a 60-mesh sieve, and set it aside; the specific steps of extracting buckwheat bran flavonoids are as follows:
[0061] Enzyme hydrolysis under ultrasonic conditions: Take crushed buckwheat bran, add 40 times the amount of water to make buckwheat bran suspension, adjust the pH value to 4.0, and add enzyme amount of 0.70mg / ml to buckwheat bran to suspend Add the extraction compound enzyme to the turbid solution and mix well, and perform enzymolysis for 40 minutes under the condition of ultrasonic temperature of 40°C and power of 200W to obtain an enzymolysis solution.
[0062] (2) Centrifugal separation: centrifuge the enzymatic hydrolyzate prepared in step (1) at 3500 rpm for 15 minutes, and collect the precipitate and supernatant.
[0063] (3) Ultrasonic alcohol ext...
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