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Scenedesmus sp., CHX1 and use thereof

A technology for Scenedesmus and seeds is applied in the application field of Scenedesmus in the fields of bioenergy and purifying swine wastewater, which can solve the problems of low oil yield, low biological yield and high requirements for cultivation conditions, and achieves high oil yield and biological The effect of high yield and low requirements for culture conditions

Inactive Publication Date: 2013-03-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few domestic research reports on the coupling of advanced sewage treatment and biodiesel production based on microalgae cultivation. Screening microalgae strains suitable for sewage cultivation with high oil yield and easy conversion to biodiesel is the premise and key of this process
However, many high-oil algae species purely for the purpose of producing biodiesel, such as Chlorella and Staphylococcus brancii, do not necessarily grow normally in sewage and accumulate a large amount of oil, and these algal strains generally have high requirements for culture conditions and low biological output. Problems such as low or low oil yield

Method used

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  • Scenedesmus sp., CHX1 and use thereof
  • Scenedesmus sp., CHX1 and use thereof
  • Scenedesmus sp., CHX1 and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Isolation and identification of Scenedesmus CHX1

[0021] 1. Sample collection

[0022] In July 2011, it was collected from a wastewater pond of a farm in Xiaoshan District, Hangzhou City, Zhejiang Province containing

[0023] Water sample of green algae.

[0024] 2. Isolation and purification of Scenedesmus CHX1

[0025] Separation and purification steps: Centrifuge and concentrate the collected water samples (5000 r / min, 4°C), dilute with sterile water, pass through a 60-micron mesh protozoan sieve, and then pass through a 0.45-μm filter membrane. Rinse the filter membrane several times with sterile water, transfer the remaining cells on the filter membrane to a 12-well cell culture plate (each well contains 1ml of sterilized BG-11 culture solution, and the sterilization method is high-pressure steam sterilization at 121°C 20 minutes), placed in a light incubator for enrichment culture (the culture condition is light all day long, and the light intensit...

Embodiment 2

[0037] Example 2 Optimization of Growth Conditions of Scenedesmus CHX1

[0038] 1. Determination of Optimal Absorbance Density Value of Scenedesmus CHX1

[0039] In order to better describe the growth of Scenedesmus CHX1 and determine the optimal absorbance value of Scenedesmus CHX1, ​​the BG11 culture solution containing microalgae was scanned automatically in the range of 600-700nm. Scan results see figure 2 .

[0040] 2. Optimization and determination of the growth conditions of Scenedesmus CHX1

[0041] In order to make Scenedesmus CHX1 grow faster and better, the combination of culture mode, carbon source, glucose dosage and culture conditions was optimized on the basis of BG-11 medium.

[0042] 1. Optimal determination of culture methods

[0043] Based on the BG-11 medium, three culture methods were set up: photoautotrophic, heterotrophic and polytrophic. The photoautotrophic culture condition was light + BG-11 culture medium; the heterotrophic culture condition was...

Embodiment 3

[0054] Example 3 Growth and oil production of Scenedesmus CHX1

[0055] 1. Cultivation of Scenedesmus CHX1

[0056] Cultivate Scenedesmus CHX1 according to the optimized conditions and medium in Example 2, the culture temperature is 25°C, and the light is 40 μmol photon / m all day long 2 .s conditions for 3 days.

[0057] 2. Determination of Growth and Oil Production of Scenedesmus CHX1

[0058] 1. Biomass determination of Scenedesmus CHX1

[0059] Take the culture solution and place it in a centrifuge tube, centrifuge for 10 min (10000 r / min, 4 °C), discard the supernatant, transfer the concentrated biomass to tin foil, and freeze-dry. 2.44g of dry algae were obtained per liter of culture solution (the average biomass yield was 814.1 mg / L.d, and the range was 809.6~818.6 mg / L.day), and the biomass yield was relatively high.

[0060] 2. Determination of oil yield of Scenedesmus CHX1

[0061] Weigh a certain quality freeze-dried algae sample (weight W 0 ), add sol...

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Abstract

The invention discloses Scenedesmus sp., CHX1 and a use thereof. The Scenedesmus sp., CHX1 is separated from a pig feeding wastewater treatment tank, is named as CHX1, has a class name of Scenedesmus sp., is preserved in the China general microbiological culture collection center and has a preservation number of CGMCC No. 6649. A Scenedesmus sp., CHX1-optimized medium is obtained by preparing solutes of 1ml of an A5+Co solution, 5g of glucose, 3g of NaNO3, 0.04g of K2HPO4.3H2O, 0.075g of MgSO4.7H2O, 0.036g of CaCl2.2H2O, 0.006g of Fe(NH4)3Cl8H10O14, 0.001g of Na2-EDTA, and the balance water into 1L of a solution. The Scenedesmus sp., CHX1 is cultured into a seed culture solution by the Scenedesmus sp., CHX1-optimized medium. The seed culture solution can be used for preparation of biodiesel and treatment on pig feeding wastewater. The Scenedesmus sp., CHX1 can grow well in pig feeding wastewater, can efficiently remove nitrogen and phosphor in wastewater, has a high biological yield and an oil yield, and is suitable for biodiesel production. A fatty acid produced by the Scenedesmus sp., CHX1 has C16-C18 content more than 70%.

Description

technical field [0001] The invention relates to Scenedesmus and its application, in particular to the application of the Scenedesmus in the fields of bio-energy and purification of pig-raising wastewater. technical background [0002] Human beings are facing two major crises in the 21st century: energy crisis and water resource crisis. It is estimated that the world's crude oil reserves and natural gas reserves will be exhausted in 40 years and 64 years respectively. Therefore, it is imperative to develop economical and efficient new energy sources, among which bio-new energy is one of the main research hotspots. At the same time, 40% of the countries and regions in the world are facing the problem of water shortage, and the sustainable use of water resources is imminent. Sewage recycling as the second water source of the city provides a new way to solve the shortage of urban water resources, but the high content of nitrogen and phosphorus in the sewage is likely to cause e...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C02F3/32C02F103/20
CPCY02E50/13Y02E50/10
Inventor 程海翔田光明
Owner ZHEJIANG UNIV
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