Device and method for online deduction of amino acid disulfide and determination of amino acid

A technology for disulfides and amino acids, which is applied to the device and field of online reduction of amino acid disulfides and determination of amino acids, which can solve the problems of being easily affected by interfering substances, reduced sensitivity, and many operating steps, and achieves high practical application prospects and reduction The effect of fast process and simple production process

Inactive Publication Date: 2013-03-27
BEIJING UNIV OF CHEM TECH
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This off-line operation is not only a waste of time, but mercapto amino acids are easily oxidized to amino acid disulfides during sample processing and subsequent analysis
Documents James L., James D. The determination of disulphide species within physical fluids. Trends Anal. Chem., 2012, 21:807-815. reported the direct simultaneous online determination of thiol amino acids and amino acid disulfides by electrochemical or mass spectrometry method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Device and method for online deduction of amino acid disulfide and determination of amino acid
  • Device and method for online deduction of amino acid disulfide and determination of amino acid
  • Device and method for online deduction of amino acid disulfide and determination of amino acid

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] The device provided by the present invention for online reduction of amino acid disulfide and determination of amino acid such as figure 1 As shown, the model of the high-pressure pump used is LC-15C, the six-port injection valve is Rodani 7725i, the high performance liquid chromatography separation column is a C-18 column, and the chemiluminescence detector is an ultra-weak chemiluminescence analyzer.

[0024] The structure of the online reduction column (4) is as figure 2 , The length of the quartz tube is 10cm and the inner diameter is 2mm. It should be rinsed with pure water before use and dried in an oven at 100°C for 3 hours; the online reduction column (4) should be rinsed with pure water for 3 hours before use.

[0025] The TCEP complex crystals are zinc(II) tris(2-carboxyethyl)phosphine complex crystals, which are synthesized according to the following steps:

[0026] The perchloric acid solution with a molar concentration of 3 mol / L is slowly added dropwise to 100 mg...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Lengthaaaaaaaaaa
The inside diameter ofaaaaaaaaaa
Login to view more

Abstract

The invention provides a device and a method for online deduction of amino acid disulfide and determination of amino acid. The device mainly comprises a high-pressure pump, a high performance liquid chromatographic column, an online reduction column, and a chemiluminescent detector. The basic technical route is as follows: a amino acid solution to be determined enters the online reduction column after being subjected to high performance liquid chromatographic column separation; and the amino acid disulfide in the solution is restored into corresponding mercaptoamino acid by tri(2-propyloic) phosphine complex crystal of zinc (II) in the online reduction column, and then enters the chemiluminescent detector to be subjected to online determination of the amino acid. The online reduction device is simple and convenient in detection method, rapid in reduction process, high in reduction efficiency, good in repeatability, and wide in application prospect, and newly restored mercaptoamino acid is prevented from being oxidized into the amino acid disulfide again in sample treatment and analysis processes.

Description

technical field [0001] The invention relates to a device and method for rapidly on-line reduction of amino acid disulfide and determination of amino acid. The device and method are suitable for rapid online determination of the content of sulfhydryl amino acid and amino acid disulfide in urine or plasma samples. Background technique [0002] The content of thiol amino acids and amino acid disulfides in biological fluids is closely related to human health. Through the determination of mercapto amino acid and amino acid disulfide content, the health of the human body can be monitored in real time. The method currently used is to reduce the amino acid disulfide to the corresponding thiol amino acid off-line, and then measure the content of various thiol amino acids, thereby determining the content of various amino acids in the sample. The literature Muhammad I.K., Zafar I. Simultaneous determination of ascorbic acid, aminothiols, and methionine in biological matrices using ion...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N1/28G01N30/02
Inventor 吕超陈清烁白守礼
Owner BEIJING UNIV OF CHEM TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap