Heterotrophic nitrifying and aerobic denitrifying bacterium and culture and application thereof

Abstract

The invention relates to a heterotrophic nitrifying and aerobic denitrifying bacterium for efficient denitrification, and the culture and application of the heterotrophic nitrifying and aerobic denitrifying bacterium. The bacterium belongs to an acinetobacter species (Acinetobacter), with the name of Acinetobacter sp.Y1, the preservation registration number of CGMCC NO.6563, the preservation institution of China Microorganism Culture Preservation Management Council General Microbiological Center and the preservation time of September 14th, 2012. The bacterium is separated from activated sludge of coking wastewater, can not only quickly remove ammonia nitrogen, but also grow under the condition that nitrite and nitrate are taken as sole nitrogen sources, and effectively removes nitrite nitrogen and nitrate nitrogen, and can be applied to nitrogen-containing wastewater treatment.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to a highly efficient denitrification heterotrophic nitrifying aerobic denitrifying bacteria, and its cultivation and application. technical background [0002] Traditional biological denitrification is an organic combination of nitrification and denitrification. The nitrification reaction refers to the NH reaction under aerobic conditions 4 + via NO 2 - Oxidation to NO 3 - process, which was first discovered by Winogradsky in 1890. The traditional nitrification reaction is completed by two kinds of microorganisms, namely ammonia oxidizing bacteria (AOB) (also known as nitrosifying bacteria) and nitrite oxidizing bacteria (NOB) (also known as nitrifying bacteria). [0003] Ammonia oxidizing bacteria and nitrite oxidizing bacteria are both aerobic bacteria that do not require organic carbon sources and are chemoautotrophic bacteria. Generally, the BOD concentration ...

AI Technical Summary

Problems solved by technology

[0005] First of all, ammonia oxidizing bacteria and nitrous acid oxidizing bacteria are chemoautotrophic bacteria. Due to the long period of time, the reactor starts slowly, which increases the cost of sewage treatment;

[0006] Second, due to the different nutritional requirements and living conditions of nitrifying bacteria and denitrifying bacteria, the entire denitrification process must be carried out in two independent reaction pools of aerobic and anoxic, which increases the volume of the reactor

Or alternate aerobic and anoxic environments in time in the same reactor, but the control of the system is also very cumbersome;

[0007] Third, denitrifying bacteria are heterotrophic bacteria, so the denitrification process requires a high concentration of organic matter. When the BOD: TN in sewage is greater than 2.86, denitrification is normal, but below this value, denitrification cannot be carried out normally.

Some processes use the reflux of nitrifying liquid to solve the problem of insufficient carbon source in the denitrification process, but the reflux of nitrifying liquid is large and the energy consumption is high;

[0008] Fourth, nitrifying bacteria will produce acid during the nitrification process, and acidic conditions can inhibit the nitrification reaction, so alkali needs to be added continuously during the nitrification process, thereby increasing the treatment cost;

[0009] Fifth, the impact resistance is weak, and high concentrations of ammonia nitrogen and nitrite will inhibit nitrification

Compared with autotrophic nitrifying bacteria, although the decomposition efficiency of heterotrophic bacteria is low, they have fast growth rate, high cell yield, low requirements for dissolved oxygen, and strong environmental adaptability. bacteria, so in some environments heterotrophic nitrification is comparable to autotrophic nitrification, or even exceeds autotrophic nitrification

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