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Antimicrobial total triterpenoid saponin of pubescent holly root and application of antimicrobial total triterpenoid saponin

A technology of holly vulgaris and total saponins is applied to the total saponins of holly vulgaris and its application field, and achieves the effects of enhancing bacteriostatic effect, good bacteriostatic effect and improving inhibitory effect.

Inactive Publication Date: 2013-05-22
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

No oral antimicrobial studies have been done so far

Method used

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  • Antimicrobial total triterpenoid saponin of pubescent holly root and application of antimicrobial total triterpenoid saponin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Extraction and precipitation: Take 1 kg of rhizome of Ilex pubescens, use 3 L of 70% ethanol to keep boiling under reflux for 3 hours, and filter; take the filtrate, recover 80% of the solvent, let it stand for precipitation, filter, and the precipitate is crude extract A. The filtrate B is set aside; the weight of the crude extract A is 255g, which contains 20% by weight of hollylicin.

[0021] (2) Crude macroporous resin: The filtrate B in step (1) was eluted with a macroporous adsorption resin, and the eluent was gradiently eluted with 30% ethanol solution and 70% ethanol solution respectively, and the 70% ethanol solution was collected. The liquid was removed, concentrated and dried to obtain a crude extract C; the weight of the crude extract C was 158g, and the weight percent content of hollylicin was 30%.

[0022] (3) Purification by silica gel column chromatography: Mix the crude extract A prepared in step (1) and the crude extract C prepared in step (2), and...

Embodiment 2

[0024] (1) Extraction: take 1 kg of rhizome of Ilex pubescens, use 10L of 60% ethanol to extract at 60°C for 5 hours, and filter; take the filtrate, recover 20% solvent, let it stand for precipitation, filter, and the precipitate is crude extract A, and the filtrate B is for later use;

[0025] (2) Crude macroporous resin: the filtrate B in step (1) was eluted with macroporous adsorption resin, and the eluent was gradiently eluted with 20% ethanol solution and 50% ethanol solution respectively, and collected 50% ethanol solution Deliquification, concentration and drying to obtain crude extract C;

[0026] (3) Purification by silica gel column chromatography: Mix the crude extract A prepared in step (1) and the crude extract C prepared in step (2), and perform silica gel column chromatography. The chloroform-methanol mixed solution of :1, the chloroform-methanol mixed solution of 5:1 carry out gradient elution, collect the chloroform-methanol mixed solution that the volume rati...

Embodiment 3

[0030] (1) Extraction and precipitation: take 1 kg of Ilex pubescens leaves, use 3 L of 70% ethanol to maintain a slight boiling heat under reflux for extraction for 3 hours, and filter; take the filtrate, recover 80% of the solvent, let it stand for precipitation, filter, and the precipitate is crude extract A. Filtrate B is set aside.

[0031] (2) Crude macroporous resin: the filtrate B in step (1) was eluted with a macroporous adsorption resin, and the eluent was gradiently eluted with 30% ethanol solution and 70% ethanol solution respectively, and the 70% ethanol solution was collected. Deliquified, concentrated and dried to obtain the crude extract C.

[0032] (3) Purification by silica gel column chromatography: Mix the crude extract A prepared in step (1) and the crude extract C prepared in step (2), and perform silica gel column chromatography. The chloroform-methanol mixed solution of 1:1 and the chloroform-methanol mixed solution of 10:1 carry out gradient elution, ...

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Abstract

The invention discloses antimicrobial total triterpenoid saponin of pubescent holly root and application of the antimicrobial total triterpenoid saponin in preparation of medicines for treating oral diseases. The total triterpenoid saponin of the pubescent holly root is prepared from rootstocks or leaves of pubescent holly root as raw materials and contains 20-50 percent by weight of pubescent holly root. The total triterpenoid saponin of pubescent holly root is simple in preparation process; and the prepared total triterpenoid saponin of pubescent holly root is good in antibacterial effect and can be subjected to further study as a main component of medicines for treating oral diseases.

Description

Technical field [0001] The present invention involves a mao Dongqing triple saponin and its application, which specifically involves a Mao Dongqing triple saponin with better antibacterial effect. Background technique [0002] Periodontal disease and dental dental diseases are under the influence of bacterial infection.Bacteria, which are closely related to the development of periodontal disease and caries, are called periodontal pathogenic bacteria and caries.Most of the bacteria in plaque are normal oral cavities, and only a few bacteria are closely related to the occurrence and development of periodontal disease.One or more advantageous bacteria can often be separated in the diseased area of periodontal diseases. They have significant toxic or pathogenicity, can interfere with the defective ability of the host through multiple mechanisms, and can cause the destruction of periodontal destruction.Potential is called peripheral pathogenic bacteria.Important periodontal pathogenic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P31/02A61P1/02A61K125/00A61K127/00
Inventor 林丽萍梁敬钰
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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