A kind of harmless treatment method of chlortetracycline solid waste
A solid waste, harmless treatment technology, applied in the field of environmental microorganisms and solid waste treatment, can solve the problems of high chlortetracycline residue, strong biological toxicity, and difficult degradation, and achieves easy control and operation of the fermentation process. Simple, acid-reducing effect
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Embodiment 1
[0030] The main component determination of embodiment 1 aureomycin fresh bacterium residue
[0031] The initial water content, pH value and chlortetracycline residual amount of the fresh bacterium residue of chlortetracycline have been measured, know that the fresh bacterium residue initial water content of chlortetracycline≤80% (60-80%) (weight ratio ), the pH is 2.0-3.0, and the aureomycin content is 0.5-2.5% (on a dry basis).
[0032] Determination method of water content: adopt 105 ℃ drying method.
[0033] pH value measurement method: use acidity meter to measure.
[0034] Determination method of aureomycin content: high performance liquid chromatography (HPLC) method. The specific operation is: accurately weigh 2.0000g of bacteria residue, grind it, add 20mL of acetone extract, ultrasonicate for 30min, and filter with a 0.22μm filter membrane. The content of aureomycin in the filtrate was measured by high performance liquid chromatography and the degradation rate was ...
Embodiment 2
[0037] A kind of chlortetracycline slag biological treatment method of embodiment 2
[0038] The method mainly includes the following steps:
[0039] 1. Degradation strain selection and strain activation culture
[0040]According to the characteristics of the low pH value of the fresh bacterium residue of aureomycin and the high residual amount of aureomycin, Trichoderma harzii (Hypocrea lixii), Penicillium oxalicum (Penicillium oxalicum), Penicillium citrinum (Penicillium citrinum) and Penicillium punctatus (Penicillium spinulosu) and other aureomycin-degrading strains, such as Hypocrea lixii LJ245 (CGMCC NO.5753), Penicillium citrinum LJ318 (CGMCC NO.5850), Penicillium oxalicum LJ302 (CGMCC NO.7019) and Penicillium spinulosu LJ220 (CGMCC NO.5754) as preferred strains. Prepare Martin's Medium or Potato Glucose Medium, and activate the strains. The culture conditions are 28-30°C, 150-180r / min shaking culture for 24-48h, and then transfer to a larger container to continue the...
Embodiment 3
[0061] A kind of chlortetracycline slag biological treatment method of embodiment 3
[0062] The method mainly includes the following steps:
[0063] 1. Strain preparation
[0064] Inoculate the microbial strains that degrade chlortetracycline, such as Penicillium punctatus, Penicillium citrinum, Trichoderma harthorne and Penicillium oxalicum, in Martin's medium or potato dextrose medium, placed at 28-30°C, 150 Cultivate for 24-48h under the condition of -170r / min shaking, and then transfer to a container with a larger volume to continue culturing, and operate in this way for 2-4 generations. The preparation method of Martin's medium and potato glucose medium is the same as that in Example 2.
[0065] 2. Pretreatment of fungus residue raw materials
[0066] Break the fresh chlortetracycline residue from bulk particles into 1-5mm sized particles. Then put it into a high-pressure steam sterilizer and treat it at 121°C for 20min-30min. The bacteria residue without high tempe...
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