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Method for preparing brassica oleracea var. caulorapa by using biological tissue culture method

A technology of biological tissue and culture method, which is applied in the biological field to achieve the effects of good water retention, increased germination and multiplication rates, and sufficient nutrition

Inactive Publication Date: 2013-06-12
康建国
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there have been few reports on the tissue culture of kohlrabi at home and abroad. This technology establishes a complete regeneration system of kohlrabi through the determination of medium components, hormone ratios and environmental factors, which is a great contribution to the large-scale production of kohlrabi. Large-scale rapid propagation and its genetic transformation research laid the foundation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Carefully scrub the surface of fresh kohlrabi leaves with detergent, rinse with running water for 10 minutes, disinfect with 75% ethanol for 30 seconds, disinfect with 0.2% mercury liter for 5 minutes, add 2 drops of Tween 80, and then use 0.2% Na 2 S 2 0 3 Wash with sterile water 3 times;

[0023] 2. Cut the leaves of sterilized kohlrabi into 0.8cm 2 As explants, they were inoculated on the medium for callus induction, and the medium composition was MS+3% sucrose+NAA1.Omg / L+6-BA1.0mg / L+2.4-D 0.01mg / L Combination, add hydrolyzed casein 100mg / L+0.2% polyglycerol palmitate simultaneously as anti-browning agent; Kohlrabi leaf is at 9 ℃, 1500Lux, 60 days callus induction rate is 40%, can utilize callus as 20%;

[0024] 3. After the callus is formed, transfer to the proliferation medium. The composition of the proliferation medium is MS+NAA 1.0mg / L+IBA 1.0mg / L+6-BA 0.05mg / L to induce bud formation and cluster bud proliferation , the multiplication ratio of cluster bu...

Embodiment 2

[0028] 1. Carefully scrub the surface of fresh kohlrabi leaves with washing powder, rinse with running water for 10 minutes, disinfect with 75% ethanol for 30 seconds, disinfect with 0.1% mercury liter for 7 minutes, add 3 drops of Tween 80, and then use 0.2% Na 2 S 2 0 3 Wash with sterile water 4 times;

[0029] Two, the leaves of kohlrabi after disinfection are cut into 1.Om 2 As explants, they were inoculated on the medium for callus induction, and the medium composition was MS+3% sucrose+NAA 0.05mg / L+6-BA 1.0mg / L+2.4-D O.05mg / L The combination of L, while adding hydrolyzed casein 100mg / L+0.2% polyglycerol palmitate as anti-browning agent, kohlrabi leaves at 12 ℃, 1800Lux, 40 days callus induction rate 45%, using callus twenty three%;

[0030] 3. After the callus is formed, transfer to the proliferation medium, the composition of the proliferation medium is B5+NAAO.05mg / L+IBA 1.0mg / L+6-BA 0.05mg / L, to induce the formation of buds and the proliferation of cluster buds, ...

Embodiment 3

[0034] 1. Carefully scrub the surface of fresh kohlrabi leaves with detergent, rinse with running water for 10 minutes, disinfect with 75% ethanol for 30 seconds, disinfect with 0.15% mercury liter for 5 minutes, add 2 drops of Tween 80, and then use 0.2% Na 2 S 2 0 3 Wash with sterile water 4 times;

[0035] 2. Cut the leaves of sterilized kohlrabi into 1.2m 2 As explants, they were inoculated on the medium for callus induction, and the medium composition was B5+3% sucrose+NAA 2.0mg / L+6-BA 0.05mg / L+2.4-D 0.08mg / L The combination of hydrolyzed casein 100mg / L+0.2% polyglycerol palmitate is added as an anti-browning agent at the same time, and the callus induction rate of kohlrabi leaves is 50% in 30 days at 15°C and 2000Lux, and the callus can be used 25%;

[0036] 3. After the callus is formed, transfer to the proliferation medium, the composition of the proliferation medium is B5+NAA 2.0mg / L+IBA 2.0mg / L+6-BA 1.0mg / L, to induce bud formation and cluster bud formation Prolif...

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Abstract

The invention discloses a method for preparing brassica oleracea var. caulorapa by using a biological tissue culture method, relates to a plant tissue culture method, and belongs to the technical field of biology. The method is characterized in that brassica oleracea var. caulorapa leaves are used as the explant; after disinfection treatment, inoculating on an induction calluse culture medium; after the calluse is formed, further inoculating into a differential medium so as to form an induced bud; subsequently inoculating a root culture medium; when the root of seedling grows to a certain extent, hardening and transplanting the seedling. The method has the beneficial effects that polyglycerol palmitate and Na2S203 are used as the anti-browning agent, so that the problems that heavy losses are caused or the product quality is seriously affected as the brassica oleracea var. caulorapa bolts when not being mature in the growing period, are effectively solved.

Description

technical field [0001] The invention relates to a plant tissue culture method, which belongs to the field of biotechnology, in particular to a method for preparing kohlrabi by a biological tissue culture method. Background technique [0002] Kohlrabi( Brassica oleraces var. carlorapa ), also known as kohlrabi, blue and so on. According to the analysis of nutritionists, every 500g kohlrabi contains 5.9g of protein, 11g of sugar, 4.1g of crude fiber, 3.3g of ash, 81mg of calcium, 122mg of phosphorus, 1.1mg of iron, trace carotene, 0.19mg of thiamine, riboflavin Vitamin C 0.07mg, Niacin 1.5mg, Vitamin C 152mg. Kohlrabi is very rich in vitamins, especially when taken fresh, it has a therapeutic effect on stomach problems; the vitamin C and other signature nutrients it contains have the effect of relieving pain and promoting muscle growth, and can promote the healing of gastric and duodenal ulcers; It contains a lot of water and dietary fiber, which can widen the bowel, prev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 康建国
Owner 康建国
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