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Novel melamine antigens and antibody as well as application

A melamine and antibody technology, applied in chemical instruments and methods, animal/human protein, ovalbumin, etc., can solve the problems of high cost, difficult to meet large throughput, slow analysis speed, etc., and achieve easy operation and good application prospects , the effect of less by-products

Active Publication Date: 2013-06-12
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

[0003] Since melamine is a small molecular substance, the current detection methods include liquid chromatography, liquid chromatography-mass spectrometry / mass spectrometry, gas chromatography-mass spectrometry, etc. These methods have high cost, slow analysis speed, and are difficult to meet the requirements of large throughput. Therefore, it is urgent to develop simple, fast and sensitive analytical techniques

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  • Novel melamine antigens and antibody as well as application
  • Novel melamine antigens and antibody as well as application
  • Novel melamine antigens and antibody as well as application

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Embodiment Construction

[0036] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0037] The present invention designs and synthesizes small molecule target analyte hapten, and couples with carrier protein to prepare effective artificial antigen, immunizes animals to prepare specific antibody to small molecule analyte, utilizes the specific immunological reaction of antigen antibody, thereby qualitative Quantitatively detect ultra-trace target analytes in samples, which can be used for sample determination. Its selectivity depends on the specificity of the immunological response, and its sensitivity depends on the affinity of the antibody and the detectability of the marker. Therefore, the residual amount of melamine in the sample can be quickly and accurately analyzed and detected. The key to the research of the present...

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Abstract

The invention relates to novel melamine antigens and an antibody as well as application thereof. Biguanide and ethyl chloroacetate are employed to synthesize 6-chloromethyl-1, 3, 5-triazine-2, 4-diamino under the condition of sodium methylate, then the 6-chloromethyl-1, 3, 5-triazine-2, 4-diamino reacts with 3-mercaptopropionic acid so as to obtain a hapten 3-((4, 6-diamino-1, 3, 5-triazine-2-yl)-methylthio)propionic acid, which is coupled to a protein by an active ester technique so as to obtain an artificial antigen. After animal immunization, blood taking, antiserum separation and purification on the artificial antigen, the melamine antibody can be obtained. The synthesis method is reasonable, the route is simple, and the hapten is maximally consistent with the target object. The prepared melamine antibody has high sensitivity, and can fully meet the rapid detection need of melamine.

Description

technical field [0001] The invention belongs to the technical field of immunochemistry and residue analysis of small molecule compounds; it relates to organic synthesis, immunochemistry, biochemistry and physicochemical testing technology, etc., and particularly relates to the design and synthesis of melamine haptens, artificial antigens and enzyme-labeled antigens, and the immunization of animals, specificity Preparation of antibodies and establishment of immunoassay methods, especially a new type of melamine antigen and antibody and its application. Background technique [0002] Melamine is a commonly used chemical raw material in the world. It can be condensed and polymerized with formaldehyde to produce melamine resin. It can be used in the plastics and paint industries, and can also be used as an anti-folding and anti-shrinking treatment agent for textiles. Melamine modified resin can be used as metal coating with bright color, durability and good hardness. Melamine can...

Claims

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Application Information

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IPC IPC(8): C07D251/18C07K14/77C07K16/44G01N33/543
Inventor 王硕刘冰史小文
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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