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Method of extracting cynomorium songaricum polysaccharide through enzyme treatment and preparation of cynomorium songaricum polysaccharide antineoplastic agents

A technology for treating Cynomorium polysaccharide and enzyme is applied in the preparation of Cynomorium polysaccharide anti-tumor preparations, and the preparation field of Cynomorium polysaccharide can solve the problems of high operation cost, long extraction time and high extraction cost, and achieves improving immunity and improving The effect of low extraction rate and extraction cost

Inactive Publication Date: 2013-06-12
甘肃凯源生物技术开发中心有限责任公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods all study the extraction process of Cynomorium polysaccharides, providing scientific basis and experimental basis for the development of Cynomorium active polysaccharides, but there are still the following deficiencies in the promotion of the process to industrial production: ①The traditional water extraction method has a low yield, and the extraction The time is longer, the energy consumption is larger, and the extraction cost is higher; ②Microwave and ultrasonic assisted extraction of Cynomorium polysaccharide has the characteristics of short extraction time and high extraction rate, but the relative cost is relatively high, and the equipment suitable for industrial production is not yet mature ; ③Supercritical extraction Cynomorium polysaccharide has the advantages of high raw material utilization rate and high product yield, but the equipment is complicated and the operating cost is high
However, there is no report on extracting Cynomorium polysaccharides and Cynomorium polysaccharides anti-tumor preparations by enzymatic treatment

Method used

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  • Method of extracting cynomorium songaricum polysaccharide through enzyme treatment and preparation of cynomorium songaricum polysaccharide antineoplastic agents

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0021] Experimental example 1 Experiment of the inhibitory effect of Cynomorium polysaccharide on tumor cells

[0022] Test cells: colon cancer SW620 cells and cervical cancer HeLa cells

[0023] Experimental method: Take tumor cells in the logarithmic growth phase, digest them with trypsin, wash with Hank’s solution, and adjust the cells to 1×10 with RPMI1640 culture medium containing 10% calf serum. 5 / ml. Add 100 μl of the above-mentioned cell suspension to each well of the 96-well culture plate, in 5% CO 2 After culturing in an incubator at 37°C and saturated humidity for 24 hours, 100 μl of Cynomorium polysaccharide of different concentrations prepared with 10% calf serum RPMI1640 culture solution was added to each well of the experimental group, and 10% calf serum RPMI1640 was added to each well of the control group. 100 μl of culture solution, 6 replicate wells for each concentration, cultured for 72 hours. 4 hours before the termination of the experiment, take the c...

Embodiment 1

[0031] 1) Crush the dried Cynomorium and pass through a 60-mesh sieve, take 1kg of Cynomorium dry powder and put it into an extraction tank with stirring, add 15kg of distilled water, then raise the temperature to 75°C and let it stand for 40 minutes.

[0032] 2) Enzymolysis: After soaking, cool down to 35°C, add cellulase, hemicellulase, pectinase, and neutral protease, turn on the stirring device, adjust the speed to 30 rpm, and keep stirring at 35°C for 2 hours , the addition of cellulase is 3% of the mass of Cynomorium powder, the addition of hemicellulase is 5‰ of the mass of Cynomorium powder, the addition of pectinase is 1% of the mass of Cynomorium powder, and the addition of neutral protease is the mass of Cynomorium powder Quality 5‰.

[0033] 3) Enzyme inactivation and high-speed stirring: heat the soaking solution to 85°C, adjust the speed to 200 rpm, and keep it warm for 10 minutes.

[0034] 4) Concentration and alcohol precipitation: After step 4), the stirring ...

Embodiment 2

[0037] The steps are the same as in Example 1, except that cellulase and papain are added after soaking, the amount of cellulase added is 8% of the mass of Cynomorium powder, and the amount of papain added is 8% of the mass of Cynomorium powder. Obtained Cynomorium polysaccharide 71.6g, Cynomorium polysaccharide content is 84.6%. Take Cynomorium polysaccharide dry powder 200g and dissolve in 800ml purified water, add appropriate amount of correctives and preservatives according to drug quality requirements, shake well, bottle according to 10ml specification, and make Cynomorium polysaccharide anti-tumor oral liquid preparation.

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Abstract

The invention discloses a preparation method of cynomorium songaricum polysaccharide and a preparation method of cynomorium songaricum polysaccharide antineoplastic agents. The cynomorium songaricum polysaccharide mainly exits in cells or cell walls. According to the preparation method, enzyme agents are added in the extracting process, the cell walls can be degraded under a mild condition, cells without cell walls are broken through high-speed stirring, the release of polysaccharide is promoted, and the effect of improving the extracting rate is achieved. The preparation method improves the extracting rate of the cynomorium songaricum polysaccharide by 10-65% compared with a traditional water extract and alcohol precipitate method, and improves the content of the cynomorium songaricum polysaccharide by 5-40%. The preparation method is simple in process, low in extracting cost, and suitable for industrialized production. The prepared cynomorium songaricum polysaccharid extracted through the method is high in purity, and the ynomorium songaricum polysaccharide agents can be directly prepared and have various effects such as immunity enhancement, fatigue prevention and tumor prevention, and are suitable for tumor patients.

Description

technical field [0001] The invention relates to a method for extracting effective components of traditional Chinese medicines, in particular to a preparation method of Cynomorium polysaccharide and anti-tumor preparation of Cynomorium polysaccharide. Background technique [0002] Cynomorium songaricum Rupr., also known as iron stick mallet, rust iron stick, ground hair ball, Ulan high waist (Mongolian), is a single genus and single species of Cynomoriaceae (Cynomoriaceae). It is mostly parasitic on the roots of plants of the genus Nityaria L in the family Zygophyllaceae, and is a total parasitic seed plant. It is mainly produced in Gansu, Xinjiang, Qinghai, Ningxia, Inner Mongolia and other places, especially in the Hexi Corridor of Gansu. In Chinese medicine, the dried stems of the Cynomorium plant are used as the traditional Chinese medicine Cynomorium, also known as "not old medicine". The "Compendium of Materia Medica" of the Ming Dynasty records: "Locking positive temp...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P37/04A61P39/00
Inventor 罗光宏
Owner 甘肃凯源生物技术开发中心有限责任公司
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