Method of extracting cynomorium songaricum polysaccharide through enzyme treatment and preparation of cynomorium songaricum polysaccharide antineoplastic agents

A technology for treating Cynomorium polysaccharide and enzyme is applied in the preparation of Cynomorium polysaccharide anti-tumor preparations, and the preparation field of Cynomorium polysaccharide can solve the problems of high operation cost, long extraction time and high extraction cost, and achieves improving immunity and improving The effect of low extraction rate and extraction cost

Inactive Publication Date: 2013-06-12
甘肃凯源生物技术开发中心有限责任公司
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AI Technical Summary

Problems solved by technology

The above methods all study the extraction process of Cynomorium polysaccharides, providing scientific basis and experimental basis for the development of Cynomorium active polysaccharides, but there are still the following deficiencies in the promotion of the process to industrial production: ①The traditional water extraction method has a low yield, and the extraction The time is longer, the energy consumption is larger, and the extraction cost is higher; ②Microwave and ultrasonic assisted extraction of Cynomorium polysaccharid

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  • Method of extracting cynomorium songaricum polysaccharide through enzyme treatment and preparation of cynomorium songaricum polysaccharide antineoplastic agents

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0021] Experimental example 1 Cynomorium cynomorium polysaccharide's inhibitory effect on tumor cells

[0022] Test cells: colon cancer SW620 cells and cervical cancer HeLa cells

[0023] Experimental method: Take the tumor cells in the logarithmic growth phase, digest them with trypsin, wash with Hank’s solution, and adjust the cells to 1×10 with RPMI1640 medium containing 10% calf serum 5 / ml. Add 100μl of the above cell suspension to each well of a 96-well culture plate, in 5% CO 2 After culturing in an incubator at 37°C and saturated humidity for 24 hours, each well of the experimental group was added with 100μl of Cynomorium cynomorium polysaccharides prepared with 10% calf serum RPMI1640 medium, and the control group was added with 10% calf serum RPMI1640 to each well. 100μl of culture medium, 6 replicate wells for each concentration, culture for 72h. 4h before the termination of the experiment, take the culture plate, add 20μl of 5mg / mL MTT reagent to each well, continue to...

Example Embodiment

[0030] Example 1:

[0031] 1) The dried Cynomorium cynomorium was crushed and passed through a 60-mesh sieve, and 1 kg of Cynomorium cynomorium powder was put into a stirred extraction tank, 15 kg of distilled water was added, and the temperature was raised to 75° C. and allowed to stand for 40 minutes.

[0032] 2) Enzymatic hydrolysis: After soaking, cool to 35℃, add cellulase, hemicellulase, pectinase, and neutral protease, and turn on the stirring device, adjust the speed to 30 rpm, and stir at 35℃ for 2 hours. The amount of cellulase added is 3% by mass of Cynomorium cynomorium powder, the amount of hemicellulase added is 5% by mass of Cynomorium cynomorium powder, the amount of pectinase added is 1% by mass of Cynomorium cynomorium powder, and the amount of neutral protease added is Cynomorium powder The quality is 5‰.

[0033] 3) Enzyme inactivation and high-speed stirring: Heat the soaking solution to 85°C, adjust the speed to 200 rpm, and keep it warm for 10 minutes.

[0034]...

Example Embodiment

[0036] Example 2

[0037] The steps are the same as in Example 1, but the difference is that after the soaking, cellulase and papain are added, the amount of cellulase added is 8% by mass of Cynomorium powder, and the amount of papain added is 8‰ by mass of Cynomorium powder. 71.6 g of Cynomorium cynomorium polysaccharide was obtained, and the content of Cynomorium cynomorium polysaccharide was 84.6%. Dissolve 200 g of Cynomorium cynomorium polysaccharide dry powder in 800 ml of purified water, add appropriate flavors and preservatives according to the drug quality requirements, shake well, and bottle according to 10 ml specifications to make Cynomorium polysaccharide anti-tumor oral liquid preparation.

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Abstract

The invention discloses a preparation method of cynomorium songaricum polysaccharide and a preparation method of cynomorium songaricum polysaccharide antineoplastic agents. The cynomorium songaricum polysaccharide mainly exits in cells or cell walls. According to the preparation method, enzyme agents are added in the extracting process, the cell walls can be degraded under a mild condition, cells without cell walls are broken through high-speed stirring, the release of polysaccharide is promoted, and the effect of improving the extracting rate is achieved. The preparation method improves the extracting rate of the cynomorium songaricum polysaccharide by 10-65% compared with a traditional water extract and alcohol precipitate method, and improves the content of the cynomorium songaricum polysaccharide by 5-40%. The preparation method is simple in process, low in extracting cost, and suitable for industrialized production. The prepared cynomorium songaricum polysaccharid extracted through the method is high in purity, and the ynomorium songaricum polysaccharide agents can be directly prepared and have various effects such as immunity enhancement, fatigue prevention and tumor prevention, and are suitable for tumor patients.

Description

technical field [0001] The invention relates to a method for extracting effective components of traditional Chinese medicines, in particular to a preparation method of Cynomorium polysaccharide and anti-tumor preparation of Cynomorium polysaccharide. Background technique [0002] Cynomorium songaricum Rupr., also known as iron stick mallet, rust iron stick, ground hair ball, Ulan high waist (Mongolian), is a single genus and single species of Cynomoriaceae (Cynomoriaceae). It is mostly parasitic on the roots of plants of the genus Nityaria L in the family Zygophyllaceae, and is a total parasitic seed plant. It is mainly produced in Gansu, Xinjiang, Qinghai, Ningxia, Inner Mongolia and other places, especially in the Hexi Corridor of Gansu. In Chinese medicine, the dried stems of the Cynomorium plant are used as the traditional Chinese medicine Cynomorium, also known as "not old medicine". The "Compendium of Materia Medica" of the Ming Dynasty records: "Locking positive temp...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P37/04A61P39/00
Inventor 罗光宏
Owner 甘肃凯源生物技术开发中心有限责任公司
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