Method for preparing sulfated glycogen based on oysters or scallops

A technology of oyster glycogen and sulfation, which is applied in the direction of blood diseases, extracellular fluid diseases, non-central analgesics, etc., and can solve the problems of inability to develop and utilize health care products, glycogen without physiological functions, and waste of raw materials , achieve excellent immunity, facilitate detection and production, and ensure stability

Inactive Publication Date: 2013-06-26
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The target product of these two patents is the preparation of oyster glycogen products, and the hot water extraction method is used. The advantage of the hot water extraction method is that it can effectively reduce the cost, but the disadv

Method used

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Examples

Experimental program
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Example Embodiment

[0039] Example 1

[0040] Shell the scallops, keep the fleshy parts, clean them, freeze-dry them in a freeze-drying machine, and pulverize them into powder. Take dry scallop powder and add water until the mass fraction of dry powder accounts for 0.1%, and add commercial pepsin powder in an amount of 0.1% of the mass of the substrate. The solution was adjusted to pH 1 with 1M hydrochloric acid, water bath at 20°C, and enzymatic hydrolysis for 0.5 hours. After the enzymatic hydrolysis was completed, the solution was adjusted to pH 7 with 6M sodium hydroxide, and heated to 80 °C to kill the enzyme for 5 minutes (wherein, the adjustment of pH value and the step of killing the enzyme can be interchanged). Cool to room temperature. Centrifuge, discard the precipitate, and add ethanol to the supernatant to make the final ethanol concentration reach 50% by volume of the solution. After 0.5 hours, the supernatant was decanted, and the precipitate was collected, which was scallop gly...

Example Embodiment

[0043] Example 2

[0044] Shell the scallops, keep the fleshy parts, clean them, freeze-dry them in a freeze-drying machine, and pulverize them into powder. Take dry scallop powder and add water until the mass fraction of dry powder accounts for 10%, and then add commercial papain powder in an amount of 0.5% of the mass of the substrate. The solution was adjusted to pH 8 with 1M sodium hydroxide, water bath at 50°C, and enzymatic hydrolysis for 1 hour. After enzymolysis, the enzyme was heated to 90°C for 10 minutes, the solution was adjusted to pH 7 with 6M sulfuric acid, cooled, centrifuged, and the precipitate was discarded. Ethanol was added to the supernatant so that the final concentration of ethanol reached 90% by volume of the solution. After 1 hour, the supernatant was decanted, and the precipitate was collected, which was scallop glycogen after air-drying.

[0045] Preparation method of sulfation reagent: dimethylformyl and sulfur trioxide-pyridine reagent are mutu...

Example Embodiment

[0047] Example three

[0048] Shell the scallops, keep the fleshy parts, clean them, add water in the same volume, and beat the scallops into a paste to obtain the initial slurry. Take the scallop initial slurry and add water until the mass fraction of dry matter accounts for 0.5%, and then add commercial trypsin powder in an amount of 1.5% of the mass of the substrate. The solution was adjusted to pH 9 with 4M sodium hydroxide, water bath at 30°C, and enzymatic hydrolysis for 2 hours. After enzymolysis, the solution was adjusted to pH 7 with 6M sulfuric acid, and then heated to 95°C to inactivate the enzyme for 15 minutes. Cool, centrifuge, and discard the pellet. Ethanol was added to the supernatant so that the final concentration of ethanol reached 60% by volume of the solution. After 2 hours, the supernatant was decanted, and the precipitate was collected, which was scallop glycogen after air-drying.

[0049] At low temperature (0°C), the ratio of chlorosulfonic acid:p...

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Abstract

The invention discloses a method for preparing sulfated glycogen based on oysters or scallops. The method comprises the following steps of: 1, taking oysters or scallops, carrying out freeze drying on the oysters or scallops till the water content reaches 0-10 percent by mass, stirring and crushing the oysters or scallops subjected to freeze drying into powder, and adding water to the powder to prepare slurry; or taking fresh oysters or scallops, directly adding water to the fresh oysters or scallops, and homogenating to obtain slurry, wherein the mass of solid matters in the slurry accounts for 0.1-5 percent of the total mass; 2, obtaining oyster glycogen or scallop glycogen by using the slurry; 3, preparing a sulfate reagent; and 4, carrying out sulfated modification on the oyster glycogen or scallop glycogen by using the sulfated reagent. The method disclosed by the invention has the characteristics of high yield, low cost and outstanding product activity stability, wherein the yield can reach about 20 percent; and in addition, the prepared sulfate glycogen has very remarkable capabilities of improving the immunity, resisting viruses and resisting blood coagulation.

Description

technical field [0001] The invention relates to the preparation of a medicine base material, in particular to the preparation of a sulfated glycogen capable of improving immunity. Background technique [0002] In the application of medical treatment and health care products, how to improve the body's immunity is a key factor in treating cancer and improving anti-virus capabilities. However, the existing health products are either complex in composition and quite toxic to human health, or they are natural extracts, but the price is expensive and the effect is not significant. How to prepare health products and medicines that can improve immunity in a low-cost and cheap way is an urgent need for people at present. Sulfated polysaccharide is a natural compound that improves immunity, anti-virus, anti-cancer, and anti-coagulation (Li Yanhua, 2006). However, in recent years, most of the naturally extracted sulfated polysaccharides researched and developed are relatively rare, a...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P37/04A61P29/00A61P7/02A61P31/12
Inventor 朱蓓薇杨静峰赵君曹倩倩朱策姜鹏飞
Owner DALIAN POLYTECHNIC UNIVERSITY
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