Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Double-layer composite regeneration film and making method thereof

A double-layer composite and regenerative membrane technology, applied in the direction of ketone active ingredients, amide active ingredients, etc., can solve the problems that cannot meet the requirements of bone tissue and periodontal tissue defect repair, achieve controllable degradation time, and promote wound healing , the effect of good mechanical strength

Active Publication Date: 2013-07-10
SHENZHEN LANDO BIOMATERIALS
View PDF2 Cites 28 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biodegradable membrane materials in the prior art cannot meet the requirements of bone tissue and periodontal tissue defect repair in terms of mechanical properties, degradation properties, and biological activity.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: Preparation of Enhanced Dense Layer

[0055] (1) Take a collagen sample, dissolve the collagen at a certain concentration with an organic acid solvent to form a natural polymer solution, the organic acid is acetic acid or malonic acid; the pH of the solvent is 1~3, and the viscosity of the resulting mixture is 2300cp, add Nano-silver with a concentration of 80ppm, then fully stirred at room temperature for 12 hours to obtain a uniformly mixed solution for use;

[0056] (2) Take a homogeneous collagen mixed solution, in a vacuum of 0 Pa, let it stand for 1-10 minutes; remove most of the bubbles in the solution;

[0057] (3) Pour the mixed solution of collagen and nano-silver out of the bubbles into a self-made PTFE mold, then spread it out, put the mold in minus 80°C for 2 hours, and then freeze it in 0 Pa, minus 56°C Dry for 24 h to prepare a collagen sponge membrane and set it aside;

[0058] (4) Using the general preparation method of medical fabrics, wea...

Embodiment 2

[0060] Embodiment 2: Preparation of loose layer

[0061] (1) Take a collagen sample, dissolve the collagen polymer with an organic acid solvent to form a solution, and add a certain amount of hyaluronic acid and BMP growth factor; the pH of the solvent is 1~3, and the viscosity coefficient of the collagen solution is 1200cp; transparent The mass fraction of uric acid is 0.05~1.5% (m / v); the concentration of BMP growth factor is 0.00008% (m / v);

[0062] (2) Pour the prepared mixed solution into a self-made PTFE mold according to a certain amount, freeze the mold at minus 80°C for 2 hours, and then freeze-dry it at 0 Pa and minus 56°C for 24 hours to prepare out of the loose layer.

Embodiment 3

[0063] Example 3 Strengthening the cross-linking and compounding of dense layer and loose layer

[0064] (1) Put the prepared dense layer and loose layer into a vacuum drying oven, vacuumize, under the state of vacuum of 0.1 Pa, high temperature crosslinking for 12~30 h; the temperature of high temperature crosslinking is 100~130℃ .

[0065] (2) Put the loose layer after high-temperature cross-linking into the container, add a cross-linking agent, the concentration of the cross-linking agent is 0.1~1.5%, cross-linking for 12 hours, selected from glutaraldehyde, genipin, carbodiimide , epoxy compounds and other cross-linking agents in one or more.

[0066] (3) Clean the chemically cross-linked loose layer. The conditions are: soak in PBS solution for 1-3 hours, then soak in 20-60% alcohol solution for 0.5-2 hours, then soak in normal saline for 24 hours, and wash continuously. Finally, wash with distilled water for 24 hours, and change the water constantly; remove the residua...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Diameteraaaaaaaaaa
Thicknessaaaaaaaaaa
Login to View More

Abstract

The invention relates to a double-layer composite regeneration film and a making method thereof. The regeneration film comprises a compact layer and a loose layer, the compact layer is formed through compounding a degradable high-molecular fiber web with a collagen film, or with collagen and nano-silver particles, or with collagen and a medicine having a treatment effect, and the loose layer is formed by treating one or more selected from chitosan, silk fibroins, keratoprotein and collagens as a matrix material. The preparation method mainly comprises a step of preparing the fiber strengthened compact layer, a step of preparing the loose layer, and a step of preparing a guide film through compounding. The regeneration film has the advantages of good biocompatibility and mechanical performances, biodegradability, low immune rejection response, simple operation and the like, and can be widely used for restoring tissues, especially bone tissues and periodontal tissues.

Description

technical field [0001] The invention relates to a guided tissue regeneration membrane in the field of biomedical materials and a preparation method thereof. The material can be used for repairing defective tissues, especially bone tissue and periodontal tissue. Background technique [0002] Guided tissue regeneration (guided tissue regeneration, GTR) has been widely used in periodontal surgery, and is considered to be an effective means of causing physiological regeneration of periodontal tissue. At present, the membrane materials used for guided tissue regeneration are divided into two categories: degradable membrane materials and non-degradable membrane materials. Although non-degradable membrane materials have good biocompatibility and mechanical properties and have achieved certain curative effects, they cannot be absorbed by tissues and require secondary surgery to remove them, which increases the pain of patients, so they are not accepted by patients. Biodegradable me...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/12A61K31/16
Inventor 佘振定江宇
Owner SHENZHEN LANDO BIOMATERIALS
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com