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A kind of nanoparticle and polymercapto copolymer complex and preparation method thereof

A nanoparticle, silver nanoparticle technology, applied in pharmaceutical formulations, inactive medical preparations, inorganic inactive ingredients, etc., can solve the problems of complex design, lack of universality of nanoparticle modification, etc., and achieve good colloid The effect of stability, resistance to plasma protein adsorption, and strong practicability

Active Publication Date: 2014-10-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently very few polythiol ligands are not only complicated to design but also not universal for nanoparticle modification.

Method used

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  • A kind of nanoparticle and polymercapto copolymer complex and preparation method thereof
  • A kind of nanoparticle and polymercapto copolymer complex and preparation method thereof
  • A kind of nanoparticle and polymercapto copolymer complex and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of gold nanorods with a size of 40 × 10 nanometers: first prepare gold nanoseeds, add chloroauric acid (0.085g / L) in 10mL of three-distilled water, cetyltrimethylammonium bromide (CTAB, 36.45g / L), under vigorous stirring, quickly add NaBH 4 Aqueous solution (0.0132g / L), stirring for 5 minutes, standing for 4 hours, can obtain gold nano-seeds. Add chloroauric acid (0.17g / L), cetyltrimethylammonium bromide (CTAB, 36.45g / L), AgNO 3 (0.017g / L), ascorbic acid (0.09715g / L) and 1.5mL of the above seed solution, stirred for 5 minutes, and stood for 24h to obtain an aqueous solution of gold nanorods with a size of 40×10 nanometers.

[0036] Add the aqueous solution of polymercapto copolymer with the structure shown in the following formula to the gold nanorod solution prepared above to obtain a mixed solution, so that the gold atom concentration in the mixed solution reaches 0.5 mM, and the mercapto group concentration of the polymercapto copolymer reaches 1 mM, and ...

Embodiment 2

[0046] Preparation of gold nanospheres with a size of 16 nanometers: add chloroauric acid to 50mL of boiling three-distilled water to make the concentration of chloroauric acid reach 1mM, under vigorous stirring, quickly add 5.8mL of 38.8mM trisodium citrate aqueous solution, and boil under stirring In 10 minutes, an aqueous solution of gold nanoparticles with a size of about 16nm can be obtained.

[0047] Add the polymercapto copolymer aqueous solution with the structure shown in the following formula into the gold nanosphere solution prepared above to obtain a mixed solution, so that the gold atom concentration in the mixed solution reaches 0.5 mM, and the mercapto group concentration of the polymercapto copolymer reaches 0.5 mM, and stir for 24 Hours, centrifugal separation to obtain gold nanospheres and polythiol copolymer complexes.

[0048]

[0049] like Figure 4 Shown is the transmission electron micrograph of the gold nanosphere modified by the copolymer, showing ...

Embodiment 3

[0056] Preparation of silver nanoparticles with a size of 15 nm: add silver nitrate to 60 mL of three-distilled water to make the concentration of silver nitrate reach 0.25 mM, then add trisodium citrate to make the concentration reach 0.25 mM, stir vigorously, add 1.8 mL of 10 mM Sodium borohydride ice solution, stirred for 1 hour, can obtain the silver nanoparticle aqueous solution with a size of about 15nm.

[0057] Add the polymercapto copolymer aqueous solution with the structure shown in the following formula into the silver nanoparticle solution prepared above to obtain a mixed solution, so that the silver atom concentration in the mixed solution reaches 0.5mM, and the mercapto group concentration of the polymercapto copolymer reaches 0.5mM, and stir for 24 hours , centrifuged to obtain silver nanoparticles and polymercapto copolymer composites.

[0058]

[0059] The silver nanoparticles modified by the multimercapto copolymer were compared with the silver nanopartic...

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Abstract

The invention discloses a compound of nanoparticles and a polythiol copolymer. The compound is formed by the nanoparticles and the polythiol copolymer wrapped on the surface of the nanoparticles. Due to the multi-point key bonding effect of the polythiol with the surface of the nanoparticles, the compound is strong in combined stability, and is good in stability under the competitive condition of high thiol molecules with strong acidity. The compound is good in biocompatibility, thereby effectively preventing plasma protein from being adsorbed. The compound is good in dispersion stability in blood plasma, thereby effectively preventing the endocytosis of macrophage. The compound can be effectively enriched in a tumor part, thereby realizing the passive targeting of the tumor. Therefore, the compound has a wide application prospect in the field of cancer diagnosis and therapy. The invention further discloses a preparation method for the compound of the nanoparticles and the polythiol copolymer. The method is simple in copolymerization, unique, simple and easy to operate, wide in application range and strong in implementation.

Description

technical field [0001] The invention relates to a biomedical nanoparticle compound and a preparation method thereof, in particular to a nanoparticle-polymercapto copolymer compound and a preparation method thereof. Background technique [0002] At present, nanotechnology is more and more widely used in the field of biomedicine, especially in the diagnosis and treatment of major human diseases. Existing nanomaterials such as noble metal nanoparticles and inorganic semiconductor quantum dots have great application prospects in cancer imaging diagnosis, drug delivery, and photothermal therapy. [0003] However, the human body is a rather complex system, and nanomaterials must overcome numerous obstacles in the body if they want to perform their target functions in the human body. Generally, when nanomaterials without special modification on the surface enter the human blood system, they will inevitably adsorb plasma proteins, or even aggregate, and then be recognized and phago...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/32A61K47/02A61K47/04
Inventor 计剑汤建国刘湘圣王海波金桥陈杨军邵瑾燕郑怡
Owner ZHEJIANG UNIV
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