Preparation method of anti-oxidation water-in-oil microemulsion
A technology of microemulsion and oxidized oil, which is applied in emulsion delivery, cosmetic preparations, pharmaceutical formulations, etc. It can solve the problems of alteration, teratogenicity, and carcinogenicity, and achieve the effects of increasing added value, improving quality, and extending shelf life
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Embodiment 1
[0027] 1) Preparation of peach kernel protein isolate:
[0028] 1.1) Soak the peach kernels and peel them, dry and crush them at 35°C, defatted with petroleum ether 4 times, evaporate the solvent and pass through a 60-mesh sieve, and dry at low temperature to obtain peach kernel defatted powder, in which 1g of peeled peach kernels is added to each degreasing 6mL petroleum ether;
[0029] 1.2) Mix peach kernel defatted powder with distilled water at a mass ratio of 1:10, adjust the pH to 8.0 with 0.5mol / L NaOH solution, stir and extract at room temperature for 1 hour, centrifuge at 4000r / min for 20min, collect the supernatant, and combine the resulting precipitate with distilled water Mix at a mass ratio of 1:5, stir and extract at room temperature for 1 hour, centrifuge at 4000r / min for 20min, collect the supernatant, mix the resulting precipitate with distilled water at a mass ratio of 1:5, stir and extract at room temperature for 1h, centrifuge at 4000r / min for 20min, and collect...
Embodiment 2
[0040] 1) Preparation of peach kernel protein isolate:
[0041] 1.1) Soak the peach kernels and peel them, dry and crush them at 35°C, defatted with petroleum ether 4 times, evaporate the solvent and pass through a 60-mesh sieve, and dry at low temperature to obtain peach kernel defatted powder, in which 1g of peeled peach kernels is added to each degreasing 6mL of petroleum ether;
[0042] 1.2) Mix peach kernel defatted powder with distilled water at a mass ratio of 1:10, adjust the pH to 8.0 with 0.5mol / L NaOH solution, stir and extract at room temperature for 1 hour, centrifuge at 4000r / min for 20min, collect the supernatant, and combine the resulting precipitate with distilled water Mix at a mass ratio of 1:5, stir and extract at room temperature for 1 hour, centrifuge at 4000r / min for 20min, collect the supernatant, mix the resulting precipitate with distilled water at a mass ratio of 1:5, stir and extract at room temperature for 1h, centrifuge at 4000r / min for 20min, and coll...
Embodiment 3
[0052] 1) Preparation of peach kernel protein isolate:
[0053] 1.1) Soak the peach kernels and peel them, dry and crush them at 35°C, defatted with petroleum ether 4 times, evaporate the solvent and pass through a 60-mesh sieve, and dry at low temperature to obtain peach kernel defatted powder, in which 1g of peeled peach kernels is added to each degreasing 6mL petroleum ether;
[0054] 1.2) Mix peach kernel defatted powder with distilled water at a mass ratio of 1:10, adjust the pH to 8.0 with 0.5mol / L NaOH solution, stir and extract at room temperature for 1 hour, centrifuge at 4000r / min for 20min, collect the supernatant, and combine the resulting precipitate with distilled water Mix at a mass ratio of 1:5, stir and extract at room temperature for 1 hour, centrifuge at 4000r / min for 20min, collect the supernatant, mix the resulting precipitate with distilled water at a mass ratio of 1:5, stir and extract at room temperature for 1h, centrifuge at 4000r / min for 20min, and collect...
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