Chitin membrane and application of chitin membrane in ophthalmologic treatment

A chitin and medical device technology, applied in the field of medicine and biology, can solve the problems of cell shedding, failure to achieve clinical research, poor compatibility between rabbit corneal cells and chitosan film, etc., and achieves good biocompatibility and good mechanical properties. The effect of strength and good flexibility

Inactive Publication Date: 2013-08-14
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2000, Elizabeth used collagen sponge as a carrier to culture corneal epithelial cells, stromal cells, and endothelial cells respectively, and found that corneal cells could grow and migrate on the collagen sponge, and could show the cell morphology and characteristics under normal physiological conditions, but the collagen sponge culture The tissue transparency formed by stromal cells can only reach 50% of the normal cornea, which cannot meet the requirements of clinical research
In 2001, Yao Ziang et al. studied the culture of rabbit corneal endothelial cells on chitosan film, chitosan and chondroitin sulfate blend film, and found that the compatibility between rabbit corneal cells and chitosan film was poor, and after 24 hours of culture Afterwards, the cells began to aggregate and fall off, but the growth on the chitosan-chondroitin sulfate blend film was better, and no aggregation and fall-off phenomenon was found, but it was still not very ideal. During the long-term culture, the cells fell off.
However, due to the strong hydrogen bond interaction between chitin molecules and molecules, the intermolecular structure is tight and orderly, and the structure is stable. Limit the research, development and application of chitin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The preparation of embodiment 1 chitin film 1

[0017] Weigh 3.0 g of high-purity chitin powder, add it to 100 ml of formic acid, and then add 0.5 g of NaCl as a porogen, stir and dissolve with a glass rod at room temperature to obtain a chitin colloidal solution. Cast the colloidal solution on a PE plastic plate and dry it in a fume hood at room temperature. The dried film was soaked in distilled water and washed until neutral, dried at room temperature, and sterilized by irradiation to obtain a flat chitin film 1 .

Embodiment 2

[0018] The preparation of embodiment 2 chitin film 2

[0019] Weigh 2.8 g of high-purity chitin powder, add it to 100 ml of formic acid, then add 0.2 g of hyaluronic acid, stir and dissolve with a glass rod at room temperature, and obtain a chitin-hyaluronic acid colloidal solution. Use a pipette to draw a certain amount of chitin-hyaluronic acid colloid solution, add it to the groove of a polytetrafluoroethylene rod with a curvature diameter of 14mm that can be rotated under the drive of the motor, turn on the motor, and control the rotation speed at 600-800 Rotation / min, so that the colloidal solution forms a uniform film layer in the groove, and is centrifuged and dried under natural conditions to form a curvature film. The dried membrane was soaked in distilled water and washed until neutral, dried at room temperature, and sterilized by irradiation to obtain a chitin membrane 2 with pore size and curvature.

Embodiment 3

[0020] The preparation of embodiment 3 chitin film 3

[0021] Weigh 2.2g of high-purity chitin powder, add it to 100ml formic acid, then add 0.5g chondroitin sulfate and 0.3g carboxymethyl chitosan, stir and dissolve with a glass rod at room temperature to obtain chitin-chondroitin sulfate-carboxylate Methyl chitosan colloidal solution. Use a pipette to draw a certain amount of chitin-chondroitin sulfate-carboxymethyl chitosan colloid solution, add it to the groove of a polytetrafluoroethylene rod with a curvature diameter of 14mm that can rotate under the drive of the motor, turn on the motor, The rotation speed is controlled at 600-800 rpm, so that the glue solution forms a uniform glue film layer in the groove, and is centrifugally dried under natural conditions to make a curvature film. The dried membrane was soaked in distilled water and washed until neutral, dried at room temperature, and sterilized by irradiation to obtain a chitin membrane 3 with pore size and curvatu...

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Abstract

The invention relates to an application of chitin products, and in particular relates to a chitin membrane and an application of the chitin membrane in ophthalmologic treatment, belonging to the technical field of pharmaceutical biotechnology. The chitin membrane is characterized by consisting of chitin or utilizing the chitin as a main raw material and being prepared according to the weight ratio of chitin to amino sugar of 1: (0-0.5).

Description

technical field [0001] The invention relates to the application of a chitin product, in particular to a chitin film and its application in ophthalmic treatment, and belongs to the field of medical biotechnology. Background technique [0002] Corneal disease is the main cause of blindness in my country. In terms of ocular surface diseases, such as corneal ulcers caused by pathogenic bacteria infection, ocular surface trauma caused by ophthalmic surgery or mechanical force, ocular surface burns caused by acid and alkali, etc., the treatment is mostly treated with eye drops to control infection , to promote wound healing. However, due to the short retention time of the medicine in the eyes and the frequent drips, it is difficult for patients to use the medicine, and it also causes irritation to the eyes, which may aggravate the eye diseases of some patients. In terms of deep corneal diseases, corneal ulcers, deep corneal injuries, and full-thickness corneal lacerations need t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/10A61L31/16A61L27/20
Inventor 刘万顺韩宝芹隋鲜鲜邵凯
Owner OCEAN UNIV OF CHINA
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