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Method for catalyzing inulin fructose and cassava glucose to produce high-concentration sorbitol and gluconic acid by using immobilized movement fermented monas

A technology of Zymomonas and gluconic acid, which is applied in the field of biomanufacturing and biorefining of bio-based chemicals, can solve the problems of affecting production efficiency and production cost, low concentration of sorbitol and gluconic acid, increasing costs, etc., to achieve reduction Effects of reactor usage, reduction in consumption, and cost reduction

Inactive Publication Date: 2014-12-03
EAST CHINA UNIV OF SCI & TECH
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Problems solved by technology

However, in these studies, the cells were first treated with toluene, cetyltrimethylammonium bromide or metal ions for catalytic experiments, and it was believed that fresh untreated cells would have a large amount of sorbitol and gluconic acid in the direct catalytic production of sorbitol and gluconic acid. The by-product ethanol is produced and cannot be recycled repeatedly, which will affect the production efficiency and production cost of the whole process
At the same time, these studies obtained lower concentrations of sorbitol and gluconic acid, which increased the cost of subsequent product isolation

Method used

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  • Method for catalyzing inulin fructose and cassava glucose to produce high-concentration sorbitol and gluconic acid by using immobilized movement fermented monas

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Embodiment 1

[0029] Please see attached figure 1 , after culturing Zymomonas mobilis expressing glucose-fructose oxidoreductase for 18 hours under the condition of no ventilation, 30°C, pH 6.0, 150rpm, inoculum size 10% (v / v), the wet cells were obtained by centrifugation and purified with ultrapure Water suspension to 150g / L. Then the 12.5% ​​(w / v) PVA solution and the 0.125% (w / v) sodium alginate solution were heated and dissolved, and mixed with the cell suspension after cooling to room temperature (PVA solution and cell solution volume ratio 4:1), The mixed solution was thoroughly mixed for 1 hour under the action of magnetic stirring. Then, the mixed solution was added dropwise into a saturated boric acid solution (containing 0.3 mol / L calcium chloride) through a peristaltic pump, and stirred for 3 hours. The average diameter of the immobilized cell particles was 4 mm, and washed with a large amount of deionized water to remove the boric acid solution on the surface of the particles...

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Abstract

The invention relates to a method for simultaneously catalyzing inulin fructose and cassava glucose to produce high-concentration sorbitol and gluconic acid by using immobilized recombination movement fermented monas. The method comprises specific steps that: (1) a high-concentration equimolar inulin fructose and cassava glucose mixed hydrolysate is prepared with an enzymatic method; (2) cell immobilization of recombination movement fermented monas is carried out; and (3) the immobilized recombination movement fermented monas is used for simultaneously catalyzing fructose and glucose, such that a mixed product comprising high-concentration sorbitol and gluconic acid is obtained. The method provided by the invention has the advantages that: one glucoamylase is used for synchronously hydrolyzing inulin and cassava starch, such that equimolar fructose and glucose are produced; raw material cost is low, enzyme application amount is reduced, and production process is simplified; immobilized cells are circulated in catalyzing fructose and glucose to produce sorbitol and gluconic acid, such that catalyst dose is reduced, and continuous production of cell catalyst is avoided; the high-concentration sorbitol and gluconic acid mixed liquid is obtained, such that subsequent product separation cost is reduced.

Description

【Technical field】 [0001] The invention relates to the field of bio-manufacturing and bio-refining of bio-based chemicals, specifically, using inulin and tapioca starch as raw materials, using recombinant Zymomonas mobilis to catalyze equimolar concentration of inulin fructose and tapioca starch to produce high Method of Concentration Sorbitol and Gluconic Acid. 【Background technique】 [0002] Sorbitol is a widely used chemical raw material, mainly used in the production and preparation of sweeteners, wetting agents, conditioners, softeners, etc. The current industrial production technology of sorbitol is to use 50% D-glucose syrup catalytic hydrogenation process, but this chemical method to produce sorbitol is a process with high energy consumption, high recovery cost and environmental pollution. The biological method to produce sorbitol is mainly to use the glucose-fructose oxidoreductase in the periplasmic space of Zymomonas mobilis to convert fructose and glucose to gene...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12P7/58C12R1/01
Inventor 鲍杰安克红胡凤仙
Owner EAST CHINA UNIV OF SCI & TECH
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