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Tyrosinase Inhibitory Activity and Application of Polysaccharides from Radix Polygoni Multiflori

The technology of tyrosinase diphenolase and Baishouwu is applied in the directions of organic active ingredients, preparations for skin care, medical preparations containing active ingredients, etc., and achieves the effects of good safety, obvious effect and low production cost.

Active Publication Date: 2016-02-24
YANTAI BAOFULING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report that the polysaccharide extracted from Baishouwu medicinal material has the activity of inhibiting tyrosinase and its related uses.

Method used

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  • Tyrosinase Inhibitory Activity and Application of Polysaccharides from Radix Polygoni Multiflori
  • Tyrosinase Inhibitory Activity and Application of Polysaccharides from Radix Polygoni Multiflori
  • Tyrosinase Inhibitory Activity and Application of Polysaccharides from Radix Polygoni Multiflori

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation of Baishouwu total polysaccharides: Baishouwu tuberous root was pulverized with a pulverizer, 20 g Baishouwu powder was extracted with ethanol for many times, the residue was air-dried, and leached with boiling water according to the ratio of material to liquid 1:20, and the extract was α- Amylase and saccharifying enzyme were used to remove starch, and papain and Sevag reagent (chloroform: n-butanol was 5: 1) were used to remove protein. The supernatant was concentrated to a certain volume, and ethanol was added for precipitation. Ethanol, acetone, ether washing, vacuum drying to obtain Baishouwu polysaccharide 1.39g.

Embodiment 2

[0021] Preparation of Baishouwu total polysaccharide: Baishouwu tuberous root is pulverized with a pulverizer, passed through a 20-mesh sieve, Baishouwu powder is extracted with ethanol for several times, the residue is air-dried until there is no alcohol smell, and boiling water is carried out according to the ratio of material to liquid 1:20 Extraction, water bath for 2 h, centrifugation at 4000 r / min for 5 min, and suction filtration of the supernatant. Add amylase to the suction-filtered supernatant, adjust to PH=6, take a water bath at 85°C for 1.5h, use I 2 / KI detected to I 2 Does not discolor. Add saccharifying enzyme (45°C in advance), adjust the pH to 6, place in a water bath at 60°C for 1 h, remove the residue by suction filtration, collect the extract, and concentrate to a small volume. The above-mentioned small-volume concentrate was added with a mixture of chloroform and methanol (5:1) at a ratio of 1:3, and centrifuged at 3500 r / min for 3 min. The precipitatio...

Embodiment 3

[0023] The experimental method for the inhibition of tyrosinase activity by total polysaccharides of Baishouwu:

[0024] Reagents: Mushroom tyrosinase (EC.1.14.18.1, specific activity is 5370U / mg) and levodopa (1-dopa) are products of Sigma company, and hydroquinone (AR grade) is products of Beijing Zizhu Pharmaceutical Co., Ltd. , and other reagents (AR grade) are products of Sinopharm Group Shanghai Chemical Reagent Company.

[0025] Instruments: AL104 electronic analytical balance, manufactured by METTLER TOLEDO (Shanghai) Instrument Co., Ltd.; MuitiskanMK3 multifunctional microplate reader, manufactured by Shanghai Rebo Analytical Instrument Co., Ltd.

[0026]The tyrosinase was dissolved in phosphate buffer to prepare a 500U / mL solution, which was subpackaged and frozen, and was diluted with phosphate buffer to 25U / mL before the experiment. Before the experiment, an appropriate amount of levodopa was weighed, dissolved in phosphate buffer to prepare a 15 mmol / L solution, ...

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Abstract

The invention relates to tyrosinase inhibitory activity of total polysaccharide which is extracted and separated from traditional Chinese medicine bunge auriculate root and clarifies a new application of the total polysaccharide in the fields of medicines, food and daily chemicals. Influence of the total polysaccharide on tyrosinase diphenol enzyme activity is detected by virtue of an enzymatic reaction, experimental results prove that the total polysaccharide of bunge auriculate root has strong inhibiting effect on mushroom tyrosinase diphenol enzyme activity, IC50 is 31.8mug / ml, and an inhibition mechanism of the total polysaccharide belongs to reversible competitive inhibition. The total polysaccharide of bunge auriculate root can be used for preparing a medicine used for treating hyperpigmentation diseases, can be used for preparing health care food and cosmetics which are used for preventing skin from being sunburnt, whitening and removing freckles and can be also used for preparing a fruit and vegetable colour retention agent which can prevent brown stain of food. The application of the total polysaccharide of bunge auriculate root based on tyrosinase inhibition activity has the characteristics of nature, high efficiency, safety and no toxicity.

Description

technical field [0001] The invention relates to the tyrosinase inhibitory activity of Baishouwu polysaccharide and its application. The invention relates to the total polysaccharide extracted and separated from the traditional Chinese medicine Baishouwu, which has the activity of inhibiting tyrosinase, and clarifies its preparation method and its new application in the fields of medicine, food and daily chemical industry. Background technique [0002] Tyrosinase is a copper-containing polyphenol oxidase, which is the rate-limiting enzyme in the biosynthesis of melanin, catalyzing the hydroxylation of tyrosine to dopa, the oxidation of dopa to dopaquinone, and the 5,6 -Dihydroxyindole (DHI) oxidation to 5,6-indolequinone in three steps. Its expression and activity determine the amount and speed of melanin production. Excessive reaction can cause hypermelanosis, such as freckles, chloasma, etc. Excessive production of melanin is the root cause of the formation of chloasma; t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A23L33/00A23L5/41A61K8/73A61Q19/02A61Q19/00A61Q17/04A61P17/00
Inventor 王晓岚姚文杰苑亮
Owner YANTAI BAOFULING BIOTECH CO LTD
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