Regenerated silk fibroin solution and preparation method thereof

Abstract

The invention discloses regenerated silk fibroin solution and a preparation method thereof. The specific preparation method comprises the following steps of: taking phosphoric acid, formic acid and water as a main solvent (having an acid solution concentration of 50-100%), and adding inorganic salt having a concentration of 0.1-50% as a cosolvent, and preparing dissolution solution; and placing degummed silk in the dissolution solution, wherein the concentration of degummed silk is 0.1-50%; and dissolving the degummed silk for 0.5-5 hours at 10-80 DEG C, so as to obtain the regenerated silk fibroin solution. The preparation method disclosed by the invention is simple and short in flow; and the main component in the regenerated silk fibroin solution obtained by the preparation method is silk fibroin fibril, and the regenerated silk fibroin material prepared from the solution has excellent mechanical performance and is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of natural polymer materials, and in particular relates to a regenerated silk fibroin solution and a preparation method thereof. The method is simple and efficient, and can be widely used in biology and medicine. Background technique [0002] Natural silk is fibrous, and biomedical materials require diversity and plasticity in the shape of scaffolds. Therefore, the regeneration and processing of natural silk fibers is a necessary step to broaden its application range. At present, silk protein can be processed into ropes, re-growth silk, nanofiber membranes, silk membranes, porous membranes, hydrogels, etc. after degumming and dissolving. Among them, the dissolution of degummed silk is a very critical step, which affects the subsequent silk fibroin. The properties of materials, especially the mechanical properties, are related to the application properties of silk fibroin materials. [0003] Although high-concentratio...

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Problems solved by technology

However, after dissolving silk with a high-concentration neutral salt solution, it is necessary to place the silk fibroin salt solution in a dialysis bag for long-term dialysis (generally more than 3 days) to obtain a regenerated silk fibroin aqueous solution and dry it to form a film Or lyophilized into a porous scaffold, the process is more complicated, the cycle is long, the yield of silk is low, and the mechanical properties of the regenerated silk fibroin scaffold are poor, especially in the brittleness and low strength of the processed material, so Greatly limit the industrial production and wide application of silk protein regeneration

[0004] On the other hand, inside the natural silk, there is a wire structure with a diameter of tens of nanometers in the silk gland before silkworm spinning, and this nanostructure plays a key role in the formation of silk and the excellent mechanical properties of silk; however, the existing technology In the natural silk regeneration process, the dissolution step severely damaged the nanostructure, which became one of the main reasons why it was difficult for the regenerated silk fibroin scaffold to obtain the excellent mechanical properties of natural silk.

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