Probiotics microcapsule product and preparation and application thereof

A technology of microcapsules and probiotics, applied in the field of probiotic microcapsules products, can solve the problems of limited gastric acid barrier effect, achieve high embedding rate, high activity, and significant tolerance

Active Publication Date: 2013-10-23
于炜婷
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the barrier effect of the probiotic microcapsules prepared by the above materials on gastric acid is very limited.

Method used

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  • Probiotics microcapsule product and preparation and application thereof
  • Probiotics microcapsule product and preparation and application thereof
  • Probiotics microcapsule product and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Calcium alginate gel microspheres embedded with Lactobacillus rhamnosus were prepared by co-directional / axial flow technology under sterile conditions, and the cell seeding density was 1×10 7 / ml calcium alginate gel microspheres.

[0034] 2) Microspheres are immersed in chitosan (chitosan molecular weight 50kDa, deacetylation degree 98%) in the solution (concentration 5g / L), the volume ratio of microspheres and chitosan solution is 1: 10, react for 20 minutes, physiological After washing with brine, AC microencapsulated Lactobacillus was prepared.

[0035] 3) The AC microcapsules were liquefied with 55 mM sodium citrate solution for 10 minutes to prepare AC microencapsulated Lactobacillus in a liquid environment inside.

[0036] 4) Put AC microencapsulated Lactobacillus into MRS basal medium, culture at 37°C for 24 hours until the end of the logarithmic growth phase, and the cell density in the microcapsules reaches 5×10 11 / ml microcapsules ( figure 1 ).

[003...

Embodiment 2

[0041] 1) Under sterile conditions, in an anaerobic workstation, calcium alginate gel microspheres embedded with Bifidobacterium longum were prepared by emulsification-internal gelation technology, and the cell seeding density was 1×10 7 / ml calcium alginate gel microspheres.

[0042] 2) Microspheres are immersed in chitosan (chitosan molecular weight 100kDa, deacetylation degree 90%) in the solution (concentration 2g / L), the volume ratio of microspheres and chitosan solution is 1: 10, react for 20 minutes, physiological After washing with brine, AC microencapsulated Bifidobacterium longum was prepared.

[0043] 3) The AC microcapsules were liquefied with 55 mM sodium citrate solution for 10 minutes to prepare AC microencapsulated Bifidobacterium longum in a liquid environment inside.

[0044] 4) Put AC microencapsulated Bifidobacterium longum into the culture medium (formula (by weight): 1.67% soybean peptone, 0.83% casein peptone, 1.0% lactose, 0.5% yeast extract powder, 0....

Embodiment 3

[0049] 1) Calcium alginate gel microspheres embedded with probiotic yeast were prepared by emulsification-external gelation technology under sterile conditions, and the cell embedding density was 2×10 9 / ml calcium alginate gel microspheres ( image 3 ).

[0050] 2) Microspheres are immersed in chitosan (chitosan molecular weight 20kDa, deacetylation degree 98%) in the solution (concentration 5g / L), the volume ratio of microspheres and chitosan solution is 1: 10, react for 20 minutes, physiological After washing with brine, AC microencapsulated yeast was prepared.

[0051] 3) Add protective agent ((by weight) 3% galactose, 8% skimmed milk powder, 5% glycerol, 1.5% sodium glutamate (W / V, g / ml) to the above-mentioned microcapsules, and the balance is water ), after freeze-drying (-40°C, 12 hours), probiotic microcapsule particles were obtained, with an average particle size of 100 microns.

[0052] 4) After the above-mentioned dried microcapsules were treated with simulated g...

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Abstract

The invention relates to a probiotics microcapsule product. The probiotics microcapsule product is a spherical or irregular microcapsule with grain size of 10 to 1,000 microns and has a structure being divided into two parts, namely, a wall layer and an inner core, wherein the wall layer is composed of alginate, chitosan and a methacrylic acid-methacrylate high polymer material; and the inner core is high-density probiotics.

Description

technical field [0001] The invention relates to a probiotic microcapsule product, in particular to a probiotic microcapsule product capable of maintaining high activity of cells. Background technique [0002] The United Nations Food and Agriculture Organization (FAO) and the World Health Organization (WHO) joint expert group gave the definition of probiotics in 2001 as "living microorganisms that will give the host a beneficial effect after being taken in an appropriate amount." Since probiotic strains are derived from the intestinal tract, most of them are anaerobic bacteria or facultative anaerobic bacteria, they are very sensitive to stress environments such as oxygen, pH, temperature, and digestive tract enzymes. The activity in the medium is easily lost, resulting in a significant decrease in the viability of probiotics (a key parameter for probiotic products). Microencapsulation technology is more and more applied to the probiotic product industry because it protects ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/29A23L1/30A23L1/06A23C9/152A23C9/16A23C9/13A23K1/16A23L21/10A23L33/00
Inventor 马小军于炜婷宋慧一刘袖洞赵姗
Owner 于炜婷
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