Nutritional liquid diet containing soybean oligopeptides, and preparation method thereof
A technology of soybean oligopeptide and nutrition, applied in the field of nutritional liquid food containing soybean oligopeptide and its preparation, can solve the problems of single nutrition and uneven nutrition in liquid food, achieve enhanced nutritional intake, improve the general condition, and facilitate wound repair Effect
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Embodiment 1
[0015] In parts by weight, add 7 parts of soybean protein concentrate powder to 100 parts of magnetic stirring for 5 minutes, inactivate the obtained soybean protein concentrate emulsion in a water bath at 100 ° C for 10 minutes, quickly cool to room temperature in an ice-water bath, and then use 1mol / L NaOH solution to adjust its pH value to 7, then add alkaline protease of 3% of the concentrated soybean protein, enzymolyze in a constant temperature water bath at 60°C for 6 hours, add NaOH dropwise during this period to keep the pH value unchanged, after the enzymolysis is completed, Inactivate the enzyme in a boiling water bath for 15 minutes, quickly cool to room temperature in an ice-water bath, and centrifuge at 10,000 r for 5 minutes. The obtained supernatant is passed through an ultrafiltration membrane with a molecular weight of 1KDa. -15 Continue to purify, the column size is 2.6*80cm, the eluent is distilled water, the detection wavelength is 280nm, the flow rate is 0...
Embodiment 2
[0018] In parts by weight, add 5 parts of soybean protein concentrate powder to 100 parts of water, stir magnetically for 3 minutes, inactivate the obtained soybean protein concentrate emulsion in a water bath at 80°C for 15 minutes, and quickly cool it to room temperature in an ice-water bath , and then use 1mol / L NaOH solution to adjust its pH value to 8, then add alkaline protease at 5% of the concentrated soybean protein, and enzymolyze it in a constant temperature water bath at 60°C for 6 hours, during which NaOH is added dropwise to keep the pH value unchanged, and the enzymatic hydrolysis After the end, inactivate the enzyme in a boiling water bath for 10 minutes, quickly cool down to room temperature in an ice-water bath, and centrifuge at 10,000 r for 10 minutes. The obtained supernatant is passed through an ultrafiltration membrane with a molecular weight of 1KDa. Glycogel G‐15 continues to be purified, the column size is 2.6*80cm, the eluent is distilled water, the d...
Embodiment 3
[0021] In parts by weight, add 6 parts of soybean protein concentrate powder to 100 parts of water, stir magnetically for 5 minutes, inactivate the obtained soybean protein concentrate emulsion in a water bath at 100°C for 15 minutes, and quickly cool it to room temperature in an ice-water bath , and then use 1mol / L NaOH solution to adjust its pH value to 8, then add alkaline protease at 5% of the concentrated soybean protein, and enzymolyze it in a constant temperature water bath at 60°C for 6 hours, during which NaOH is added dropwise to keep the pH value unchanged, and the enzymatic hydrolysis After the end, inactivate the enzyme in a boiling water bath for 15 minutes, quickly cool to room temperature in an ice-water bath, and centrifuge at 10,000 r for 10 minutes. The obtained supernatant is passed through an ultrafiltration membrane with a molecular weight of 1KDa. Glycogel G‐15 continues to be purified, the column size is 2.6*80cm, the eluent is distilled water, the detec...
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