Fibroin-base artificial ligament repair material and preparation method thereof

An artificial ligament and repair material technology, applied in medical science, prosthesis, etc., can solve the problems of poor tissue compatibility, reactive exudation, rapid degeneration of carbon fiber ligament, etc., and achieve excellent biocompatibility, preparation Simple method and beneficial effect on cell adhesion

Active Publication Date: 2013-10-23
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned ligament materials have disadvantages: carbon fiber ligaments degenerate quickly, cannot provide sufficient tensile strength, poor tissue compatibility, cracked particles cause severe inflammatory reactions, and poor flexibility; Dacron ligament materials are prone to complications, such as Ligament rupture and synovitis; although the Gore-Tex ligament material is strong and the early effect of reconstruction is still good, it will degenerate over time and complications will occur; after the LAD reinforced ligament material is implanted inside the knee joint, it will cause reactive exudation, Joint fibrosis and synovitis increase the chance of infection; LARS polyester ligament cannot be degraded in the body and cannot guide cell growth to promote the formation of the body's own ligament tissue
[0005] Altman et al. used silk protein to design the tissue-engineered anterior cruciate ligament. Its 3-D spatial structure is called a steel cable structure, and its mechanical properties and fatigue resistance are sati...

Method used

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  • Fibroin-base artificial ligament repair material and preparation method thereof
  • Fibroin-base artificial ligament repair material and preparation method thereof
  • Fibroin-base artificial ligament repair material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) The weaving of the natural mulberry silk warp knitted scaffold material is woven by the RSM biaxial weft-inserted warp knitting machine. The weaving process and threading process are as follows:

[0044] a. Organization process:

[0045] GB1: 2-3 / 3-2 / 1-0 / 2-3 / 1-0 / 0-1 / 2-3 / 1-0 / /

[0046] GB2: 1-0 / 0-1 / / ×4

[0047] b. Yarn threading process:

[0048] GB1: full wear;

[0049] GB2: full wear

[0050] c. Machine number: E12;

[0051] (2) Degumming, dissolving, and dialysis of natural mulberry silk to form a silk fibroin aqueous solution with a mass fraction of 5%, placing it in a PEG solution and stirring at a low speed to concentrate to a silk fibroin aqueous solution with a mass fraction of 22%; The temperature is about 25 °C, wet spinning is carried out within a storage time of 5d, the nascent silk fibroin fibers are soaked in absolute ethanol, sealed, placed at room temperature for 24 h, and drawn in deionized water above 60 °C , the nascent fibers were drawn twic...

Embodiment 2

[0057] (1) The weaving of the warp-knitted stent material is the same as that of Example 1;

[0058] (2) Degumming, dissolving, and dialysis of natural mulberry silk to form a silk fibroin aqueous solution with a mass fraction of 5%, placing it in a PEG solution and stirring at a low speed to concentrate to a silk fibroin aqueous solution with a mass fraction of 27%; The temperature is about 25 °C, wet spinning is carried out within the storage time of 3d, the nascent silk fibroin fibers are soaked in absolute ethanol, sealed, placed at room temperature for 24 h, and drawn in deionized water above 60 °C , the nascent fibers were drawn twice and heat-set at 120 °C for 30 min. The nascent silk fibroin fibers were placed in EDC / NHS (wt / wt=2 / 1) and added to ethanol / water (V C2H5OH / V H2O = 90 / 10) in a cross-linking solution with a mass fraction of 4.5% for 24 h, stretched in deionized water above 60 °C, and then heat-set at 120 °C for 30 min to obtain EDC / NHS cross-linking The ...

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Abstract

The invention discloses a fibroin-base artificial ligament repair material and a preparation method thereof. The ligament repair material comprises stents and laid-in yarns, wherein the stents are produced by carrying out biaxial laid-in warp knitting on natural mulberry silks; the laid-in yarns are regenerated silkworm silk fibroin filament yarns and regenerated silkworm silk nanofiber yarns. The preparation method specifically comprises the following steps of: (1) preparing biaxial laid-in warp knitting stent materials; (2) preparing regenerated silk fibroin filament yarns by utilizing a dry or wet spinning method; (3) preparing and dissloving a regenerated silk fibroin film, and preparing nanometer silk fibroin fiber yarns by utilizing an electrostatic spinning method; (4) taking a pure fibroin warp knitting material as a base material, taking the regenerated silkworm silk fibroin filament yarns and the nanometer silk fibroin fiber yarns as the laid-in yarns, weaving the base material and the laid-in yarns into the warp knitting stent material to obtain the artificial ligament repair material, and sewing two sides of a ligament material into a columnar structure. The preparation method is simple and efficient, and the artificial ligament repair material prepared by utilizing the preparation method has excellent compatibility, degradability and mechanical properties.

Description

technical field [0001] The invention relates to an artificial ligament repair material, in particular to a fibroin-based artificial ligament repair material and a preparation method thereof, and belongs to the technical field of implantable medical devices. Background technique [0002] With the rise of tissue engineering, artificial ligament as an anterior cruciate ligament reconstruction material has once again attracted people's attention, providing an effective way for ligament reconstruction. Tissue engineering artificial ligament integrates the development of disciplines such as life science and engineering, using biofibers and synthetic materials as ligament scaffolds to promote cell and tissue adhesion and growth in vitro and in vivo to replace the normal anterior cruciate ligament. [0003] In the prior art, artificial ligament materials that are research hotspots and have made certain progress are permanent ligament materials, such as: carbon fiber ligament; Dacron...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/50
Inventor 吴惠英茅泳涛左保齐董启榕张锋
Owner SUZHOU UNIV
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