Fibroin-base artificial ligament repair material and preparation method thereof

Abstract

The invention discloses a fibroin-base artificial ligament repair material and a preparation method thereof. The ligament repair material comprises stents and laid-in yarns, wherein the stents are produced by carrying out biaxial laid-in warp knitting on natural mulberry silks; the laid-in yarns are regenerated silkworm silk fibroin filament yarns and regenerated silkworm silk nanofiber yarns. The preparation method specifically comprises the following steps of: (1) preparing biaxial laid-in warp knitting stent materials; (2) preparing regenerated silk fibroin filament yarns by utilizing a dry or wet spinning method; (3) preparing and dissloving a regenerated silk fibroin film, and preparing nanometer silk fibroin fiber yarns by utilizing an electrostatic spinning method; (4) taking a pure fibroin warp knitting material as a base material, taking the regenerated silkworm silk fibroin filament yarns and the nanometer silk fibroin fiber yarns as the laid-in yarns, weaving the base material and the laid-in yarns into the warp knitting stent material to obtain the artificial ligament repair material, and sewing two sides of a ligament material into a columnar structure. The preparation method is simple and efficient, and the artificial ligament repair material prepared by utilizing the preparation method has excellent compatibility, degradability and mechanical properties.

Description

technical field [0001] The invention relates to an artificial ligament repair material, in particular to a fibroin-based artificial ligament repair material and a preparation method thereof, and belongs to the technical field of implantable medical devices. Background technique [0002] With the rise of tissue engineering, artificial ligament as an anterior cruciate ligament reconstruction material has once again attracted people's attention, providing an effective way for ligament reconstruction. Tissue engineering artificial ligament integrates the development of disciplines such as life science and engineering, using biofibers and synthetic materials as ligament scaffolds to promote cell and tissue adhesion and growth in vitro and in vivo to replace the normal anterior cruciate ligament. [0003] In the prior art, artificial ligament materials that are research hotspots and have made certain progress are permanent ligament materials, such as: carbon fiber ligament; Dacron...

AI Technical Summary

Problems solved by technology

However, the above-mentioned ligament materials have disadvantages: carbon fiber ligaments degenerate quickly, cannot provide sufficient tensile strength, poor tissue compatibility, cracked particles cause severe inflammatory reactions, and poor flexibility; Dacron ligament materials are prone to complications, such as Ligament rupture and synovitis; although the Gore-Tex ligament material is strong and the early effect of reconstruction is still good, it will degenerate over time and complications will occur; after the LAD reinforced ligament material is implanted inside the knee joint, it will cause reactive exudation, Joint fibrosis and synovitis increase the chance of infection; LARS polyester ligament cannot be degraded in the body and cannot guide cell growth to promote the formation of the body's own ligament tissue

[0005] Altman et al. used silk protein to design the tissue-engineered anterior cruciate ligament. Its 3-D spatial structure is called a steel cable structure, and its mechanical properties and fatigue resistance are satisfactory (see: Gregory H. Altman, David L. Kaplan et al. Biomaterials. 23 (2002) 4131–4141), but in the process of cell culture, due to the tight structure of the cable ligament, cells can only attach to the surface of the scaffold and cannot enter the interior of the scaffold; Haifeng Liu et al. studied silk fibroin Framework-type ligament material, after the silk scaffold material is immersed in a silk fibroin solution, a 3-D spatial structure is finally formed (see: Haifeng Liu, Hongbin Fan et al. Biomaterials. 29 (2008)662–674), but the formed porous silk The stability of the material is difficult to control; Laurencin et al. used PLLA as a raw material to weave a ribbon-like scaffold material to form a 3-D spatial structure (see: Cooper JA, Lu HH et al. Biomaterials. 26 (2005)1523–1532), and its ligament Fiber bundles are cross-woven into ribbons, and the crossing angles between the middle section and both ends of the ligament are different, but the yarn structure in the middle section is crossed, which will partially affect cell adhesion

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