Preparation method of fluorescence sensor for performing high sensitivity detection on Hg(II) in water body

Abstract

The invention discloses a preparation method of a fluorescence sensor for performing high sensitivity detection on Hg (II) in a water body. The method comprises the following steps of: preparing a porous silicon coating layer with a divergent perforated structure on the surface of a template made from silicon oxide nanoparticles; and assembling fluorescent probe molecules to pore passages of surface porous silicon in a high density mode by means of sol-gel reaction. The preparation process of the fluorescence sensor comprises the following steps of: synthesizing a porous silicon layer with a surface porous structure on the surface of the silicon oxide nanoparticles; performing silanization on synthesized rhodamine 6G derivatives by the fluorescent probe molecules; assembling the fluorescent probe molecules to the pore passages of prepared surface porous silicon in a high density mode by means of silanization reaction in a methanol solution; and centrifuging, washing and drying an obtained solid material to obtain the Hg (II) fluorescence sensor with high specific surface area and high sensitivity.

Description

technical field [0001] The invention relates to the field of material science, in particular to a method for preparing a fluorescent sensor with high sensitivity for detecting Hg(II) in water. Background technique [0002] A key problem in the practical application of fluorescent detection probes is how to immobilize fluorescent probe molecules on a stable solid substrate and design them as sensors in a real sense. Porous silica materials have uniform and ordered pores, high specific surface area, and relatively good thermal stability, dispersibility, and biocompatibility, so they are widely used in catalysis, adsorption separation, microreactors, and the main body of functional assembly materials. As an excellent adsorbent, porous silica can efficiently adsorb and separate organic substances, biomolecules and inorganic substances. Therefore, the special surface properties of porous silicon can also be used to assemble fluorescent molecular probes on porous silicon to prepa...

AI Technical Summary

Problems solved by technology

These materials usually have a cage-like structure, the channel entrance is narrow and the channel orientation is parallel to the substrate surface, which is not very favorable for the assembly of fluorescent probe molecules in the channel and the detection of target analytes, mainly in the following two aspects : (1) Probe molecules transfer slowly in the channel during the assembly process and easily lead to blockage. They tend to assemble on the outer surface and the inner surface near the pore, and the modification ratio of the inner surface of the channel is relatively low, resulting in uneven distribution of guest molecules. , the pore utilization rate is low; the number of effective probe molecules is small so that the binding capacity of the target analyte is low, and the actual sensitivity is not high; (2) due to the narrow entrance of the pore, it is difficult for the target analyte ions to enter the sensing point inside the pore, so the target The binding kinetics of ions is slow, resulting in slow response time; (3) Since the target ions are difficult to bind or desorb in the pores, it is difficult to recycle the sensing material after detection, which is not conducive to the regeneration of the sensor