Preparation method of fluorescence sensor for performing high sensitivity detection on Hg(II) in water body

A fluorescent sensor and sensitive detection technology, applied in the field of material science, can solve the problems of difficult recovery and processing, low actual sensitivity, and low capacity of target analytes, so as to avoid secondary environmental pollution, high fluorescence response ability, and improve enrichment effect of ability

Inactive Publication Date: 2013-10-23
傅绪成
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  • Application Information

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Problems solved by technology

These materials usually have a cage-like structure, the channel entrance is narrow and the channel orientation is parallel to the substrate surface, which is not very favorable for the assembly of fluorescent probe molecules in the channel and the detection of target analytes, mainly in the following two aspects : (1) Probe molecules transfer slowly in the channel during the assembly process and easily lead to blockage. They tend to assemble on the outer surface and the inner surface near the pore, and the modification ratio of the inner surface of the channel is relatively low, resulting in uneven distributio

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The first step preparation of monodisperse silica sol nanospheres:

[0017] At room temperature, dissolve 1.1ml of tetraethyl orthosilicate (TEOS) in 30ml of ethanol and stir for three minutes, 1.5ml of ammonia water (28%) is quickly added to the above reaction mixture, stir vigorously for three minutes, then gently stir 18 hours. The obtained silica sol nanospheres were dispersed in toluene after three centrifugation washes.

[0018] Step 2 Preparation of superficially porous silica:

[0019] 1.0 milliliters of ethyl silicate (TEOS) and 0.7 grams of cetyltrimethylammonium bromide (CTAB, surfactant) were dissolved in 30 milliliters of ethanol, and then adding 1 milliliter of ammonia water with a mass fraction of 28% and 0.1 g of the silicon oxide nanoparticles obtained above were mixed and stirred for half an hour, and reacted at 40° C. for 18 hours. The obtained product was washed by centrifugation three times, calcined at 550°C for 6 hours to remove CTAB, and then ...

Embodiment 2

[0025] The first step preparation of monodisperse silica sol nanospheres:

[0026] With embodiment one;

[0027] Step 2 Preparation of superficially porous silica:

[0028] With octadecyltrimethylammonium chloride (STAB) as surfactant, consumption is 0.63 gram, other is the same as embodiment one, and preparation obtains surface area and pore diameter is respectively 435.36m 2 g -1 and 2.67nm;

[0029] The third step, the preparation of the fluorescent sensor on the surface porous silicon substrate:

[0030] With embodiment one;

[0031] The fourth step, Hg(II) detection test in water:

[0032] Test method is the same as embodiment one, and the result shows that this sensor is to Hg (II) in 1.0 * 10 -9 -1.0×10 -7 There is also a good linear relationship in the M range, and the lower detection limit can reach 0.1×10 -9 M (S / N=3). Other interference ions such as Pb(II), Cd(II), Zn(II), Cu(II) and Ag(I) have no or little influence on the detection signal of Hg(II).

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Abstract

The invention discloses a preparation method of a fluorescence sensor for performing high sensitivity detection on Hg (II) in a water body. The method comprises the following steps of: preparing a porous silicon coating layer with a divergent perforated structure on the surface of a template made from silicon oxide nanoparticles; and assembling fluorescent probe molecules to pore passages of surface porous silicon in a high density mode by means of sol-gel reaction. The preparation process of the fluorescence sensor comprises the following steps of: synthesizing a porous silicon layer with a surface porous structure on the surface of the silicon oxide nanoparticles; performing silanization on synthesized rhodamine 6G derivatives by the fluorescent probe molecules; assembling the fluorescent probe molecules to the pore passages of prepared surface porous silicon in a high density mode by means of silanization reaction in a methanol solution; and centrifuging, washing and drying an obtained solid material to obtain the Hg (II) fluorescence sensor with high specific surface area and high sensitivity.

Description

technical field [0001] The invention relates to the field of material science, in particular to a method for preparing a fluorescent sensor with high sensitivity for detecting Hg(II) in water. Background technique [0002] A key problem in the practical application of fluorescent detection probes is how to immobilize fluorescent probe molecules on a stable solid substrate and design them as sensors in a real sense. Porous silica materials have uniform and ordered pores, high specific surface area, and relatively good thermal stability, dispersibility, and biocompatibility, so they are widely used in catalysis, adsorption separation, microreactors, and the main body of functional assembly materials. As an excellent adsorbent, porous silica can efficiently adsorb and separate organic substances, biomolecules and inorganic substances. Therefore, the special surface properties of porous silicon can also be used to assemble fluorescent molecular probes on porous silicon to prepa...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 傅绪成谢成根吴菊
Owner 傅绪成
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