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Porcine circovirus 2 (PCV2) antibody colloidal gold immunity chromatography detection test paper and making method thereof

A technology for immunochromatographic detection and porcine circovirus, which is applied to measurement devices, analytical materials, instruments, etc., can solve problems such as the determination of the level of incapable antibodies, and achieve the effects of rapid detection, convenient portability, and simple operation.

Inactive Publication Date: 2013-10-23
WUHAN CHOPPER BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing patents on colloidal gold test paper for porcine circovirus type 2 antibody, there is generally one control line (or quality control line), and the result interpretation is only based on the color depth of the test line, and the antibody level cannot be judged

Method used

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  • Porcine circovirus 2 (PCV2) antibody colloidal gold immunity chromatography detection test paper and making method thereof
  • Porcine circovirus 2 (PCV2) antibody colloidal gold immunity chromatography detection test paper and making method thereof
  • Porcine circovirus 2 (PCV2) antibody colloidal gold immunity chromatography detection test paper and making method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Colloidal gold immunochromatographic detection test paper plane structure area map of porcine circovirus type 2 antibody figure 1 As shown, the horizontal plane of the test paper from bottom to top is: sample absorption area 1, gold label probe area 2, immobilized antibody area 3 and water absorption area 4, immobilized antibody area 3 is coated with control line C1 31, detection Line T32, control line C2 33 and control line C3 34.

[0042] Colloidal gold immunochromatographic detection test paper for porcine circovirus type 2 antibody longitudinal section structure diagram (specific materials are used to represent each area), the polyethylene plate 5 with a layer of polyvinyl chloride lining film is the support plate, and the glass fiber film 6 is the sample In the absorption area, the polyester film 7 is the gold standard probe area, the nitrocellulose membrane 8 is the solid-phase antibody area, and the absorbent filter paper 9 is the water absorption area, and the p...

Embodiment 2

[0064] Except that the coating amount of test line T is 5 μg; the coating amount of control line C1 is 0.5 μg, the coating amount of control line C2 is 5 μg, the coating amount of control line C3 is 2 μg, and the coating amount of glass fiber membrane PCV2-Cap protein is 20 μg ; The labeling amount of gold-labeled probe 1 colloidal gold-labeled porcine circovirus type 2 monoclonal antibody is 20 μg monoclonal antibody per mL colloidal gold, and the labeling amount of gold-labeled probe 2 colloidal gold-labeled rabbit IgG is 10 μg rabbit per mL colloidal gold IgG; colloidal gold-labeled porcine circovirus type 2 monoclonal antibody method: take 20mL of colloidal gold with a radius of 10nm and a concentration of 0.01% and 400μg of porcine circovirus type 2 monoclonal antibody, and pass it under the condition of pH9.0 Magnetic stirring and shaking to combine, add bovine serum albumin (BSA) and polyethylene glycol 20000 (PEG20000) as stabilizers, and make the final mass concentrati...

Embodiment 3

[0066] Except that the coating amount of test line T is 2 μg; the coating amount of control line C1 is 0.2 μg, the coating amount of control line C2 is 2 μg, the coating amount of control line C3 is 5 μg, and the coating amount of glass fiber membrane PCV2-Cap protein is 50 μg The labeling amount of gold-labeled probe 1 colloidal gold-labeled porcine circovirus type 2 monoclonal antibody is 10 μg monoclonal antibody per mL colloidal gold, and the labeling amount of gold-labeled probe 2 colloidal gold-labeled rabbit IgG is 5 μg rabbit per mL colloidal gold labeling IgG; colloidal gold-labeled porcine circovirus type 2 monoclonal antibody method: take 20mL of colloidal gold with a radius of 20nm and a concentration of 0.01% and 200μg of porcine circovirus type 2 monoclonal antibody respectively, and pass it under the condition of pH9.2 Magnetic stirring and shaking to combine, add bovine serum albumin (BSA) and polyethylene glycol 20000 (PEG20000) as stabilizers, and make the fin...

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Abstract

The invention discloses a PCV2 antibody colloidal gold immunity chromatography detection test paper and a making method thereof. The test paper comprises a sample absorption zone, a colloidal-gold probe zone, an immobilized antibody zone, a water absorption zone and a support plate, and the sample absorption zone, the colloidal-gold probe zone, the immobilized antibody zone and the water absorption zone are laid on the support plate and sequentially partially overlap with each other; the sample absorption zone is coated with PCV2-Cap proteins; the colloidal-gold probe zone is coated with a colloidal-gold probe 1 and a colloidal-gold probe 2 which are colloidal-gold labeled PCV2 monoclonal antibody and rabbit IgG respectively; and the immobilized antibody zone is sequentially coated with a contrast line C1 coated goat anti-rabbit IgG antibody, a test line T coated goat anti-pig IgG antibody, a contrast line C2 coated goat anti-rabbit IgG antibody and a contrast line C3 coated goat anti-mouse IgG antibody. The test paper has the advantages of rapid detection, high accuracy, strong specificity, and convenient carrying and operation, and is used for determining the level of the PCV2 antibody according to the deepness of the color of the contrast line.

Description

technical field [0001] The invention relates to a detection test paper and a preparation method thereof, in particular to a porcine circovirus type 2 antibody colloidal gold immune chromatography detection test paper and a preparation method. Background technique [0002] Porcine circovirus type 2 (PCV2) is the main pathogen that causes postweaning multisystemic wasting syndrome (PMWS) in weaned piglets. feature. In addition to causing PMWS, PCV2 is also associated with congenital tremor in piglets, porcine dermatitis nephritic syndrome, porcine respiratory syndrome, reproductive disorders in sows and other diseases. Since the first discovery of PMWS in Canada in 1996, the associated diseases caused by PCV2 infection have been widespread all over the world. As my country's pig industry is increasingly threatened by PCV2 infection, there is an urgent clinical need to develop a PCV2 serum antibody detection kit. The PCV genome consists of a covalently closed circular single...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N33/558
Inventor 廖园园漆世华朱薇秦伟李建刘洁谢红玲温文生冯钊
Owner WUHAN CHOPPER BIOLOGY
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