Preparation method of probiotic microbial inoculum for prevention of genseng replant diseases

Abstract

A preparation method of probiotic microbial inoculum for prevention of genseng replant diseases belongs to the microbial fermentation field, and comprises a fermentation step: producing strains of the probiotic microbial inoculum are inoculated onto an aseptic culture medium containing carbon source, nitrogen source, inorganic salts and water for fermentation, the fermentation process is aerobic mixed fermentation, the producing strains are selected from eight strains comprising Bacillus subtilis CGMCC1.1700 and the like. The probiotic microbial inoculum is a solid microbial inoculum prepared by adsorption and immobilization of a fermentation end product onto grass carbon. The preparation method solves the problem of the replant diseases existing in the ginseng planting technology in the prior art, can reuse soil in which ginseng is planted, has the advantages of low production cost, short fermentation cycle and high bacteria density, can effectively prevent soil ecological disease symptoms of dead seedling, yellow seedling, withering, flecked leaf, root rot and the like during ginseng planting, at the same time, can promote root growth, loosen soil and strengthen seedling, remove residues of pesticides and fertilizers, and has obvious effects on soil ecological restoration.

Description

Technical field [0001] The invention belongs to microbial fermentation, in particular to a preparation method of a probiotic bacterial agent capable of preventing ginseng cropping disease. Background technique [0002] Today, when ginseng is artificially planted in large quantities, due to the extensive use of chemical fertilizers and pesticides, the soil ecosystem formed by soil-microbes-plant roots is severely damaged, beneficial bacteria are inhibited, and pathogenic fungi, such as Cylindrocarpon destructans ), Phytophthora cactorum, Fusarium solani, Alternaria panax, Rhizoctonia solani and Pythium [0003] bacteria (Pythium rostratum), etc., get diffuse growth, causing frequent cropping diseases of ginseng, such as root rot, withering, yellow seedlings, mosaic leaves, black spots, etc. After ginseng is harvested, it cannot be reused. Generally, it takes more than ten years of natural restoration before ginseng can be planted again. As a result, less and less land can b...

AI Technical Summary

Problems solved by technology

The use of bacteria-fungus joint prevention of ginseng cropping disease pro-rhizobiotics and its high-density, high-activity production process have not been reported yet