Microbial reduction of graphene oxide and preparation method for graphene

A graphene and graphene suspension technology, applied in the direction of graphene, nano-carbon, etc., can solve the problems of few layers, few defects and disorder, etc., to reduce structural defects, less environmental hazards, and simple operation steps Effect

Inactive Publication Date: 2013-11-27
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Although the graphene prepared by the Shewanella method has made great progress in structure and performance relative to the chemical method (Gongming Wang, Fang Qian, Chad W. Saltikov, Yongqin Jiao.Microbial Reduction of Graphene Oxide by Shewanella .Nano Res. 2011, 4(6): 563–570), but the graphene prepared by denitrifying bacteria has fewer sheets, and less defects and disorder

Method used

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  • Microbial reduction of graphene oxide and preparation method for graphene

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Embodiment 1

[0027] The invention relates to a biologically reduced graphene oxide, which comprises the following preparation steps: The first step is pretreatment of graphene oxide. Add 50mL, 2% graphene oxide to a 1L Erlenmeyer flask,

[0028] Add deionized water to 400mL in the conical flask, in a 250W ultrasonic cleaner, ultrasonically treat at room temperature for 30min, and obtain a brown suspension of graphene oxide with better dispersion properties;

[0029] The second step is to prepare 400mL of denitrifying bacteria culture medium, adjust the pH to 7.0 with 1:9 (v / v) dilute sulfuric acid, and distribute it to four 250mL Erlenmeyer flasks, each with a volume of 100mL. Use an autoclave at 121°C with a steam pressure of 0.1MPa to sterilize for 30 minutes, put the cooled medium in the ultra-clean workbench, add 100mL graphene oxide suspension to each bottle, and inject N into each bottle for 20 minutes. 2 , to remove dissolved oxygen. Among them, the denitrifying bacteria culture m...

Embodiment 2

[0036] A kind of biological reduction graphene oxide of the present invention comprises the following steps:

[0037] The first step is graphene oxide pretreatment. Add 50mL, 2% graphene oxide into a 1L conical flask, add deionized water to 400mL in a 1L conical flask, and in a 250W ultrasonic cleaning machine, ultrasonically treat at room temperature for 30min to obtain graphene oxide with good dispersion properties brown suspension;

[0038] The second step is to prepare 100mL of denitrifying bacteria culture medium, adjust the pH to 7.0 with 1:9 (v / v) dilute sulfuric acid, and distribute it to four 250mL Erlenmeyer flasks, each with a volume of 20mL. Use an autoclave at 121°C with a steam pressure of 0.1MPa to sterilize for 30 minutes, put the cooled medium in the ultra-clean workbench, add 100mL graphene oxide suspension to each bottle, and inject N into each bottle for 20 minutes. 2 , to remove dissolved oxygen. Among them, the denitrifying bacteria culture medium incl...

Embodiment 3

[0042] A kind of biological reduction graphene oxide of the present invention comprises the following steps:

[0043] The first step is graphene oxide pretreatment. Add 50mL, 2% graphene oxide into a 1L conical flask, add deionized water to 400mL in a 1L conical flask, and in a 250W ultrasonic cleaning machine, ultrasonically treat at room temperature for 30min to obtain graphene oxide with good dispersion properties brown suspension;

[0044] The second step is to prepare 400mL of denitrifying bacteria culture medium, adjust the pH to 7.0 with 1:9 (v / v) dilute sulfuric acid, and distribute it to four 250mL Erlenmeyer flasks, each with a volume of 100mL. Use an autoclave at 121°C with a steam pressure of 0.1MPa to sterilize for 30 minutes, put the cooled medium in the ultra-clean workbench, add 100mL graphene oxide suspension to each bottle, and inject N into each bottle for 20 minutes. 2 , to remove dissolved oxygen. Among them, the denitrifying bacteria culture medium inc...

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Abstract

The invention discloses a microbial reduction of graphene oxide and a preparation method for graphene. The method comprises the following steps: dispersing graphene oxide in water to form a graphene oxide suspending solution with better dispersion; mixing denitrifying bacteria culture medium being subjected to sterilization and cooling with the graphene oxide suspending solution; inoculating a seed solution; culturing in still standing state in anaerobic condition for a few days, removing the oxygen-containing functional group of the dispersed graphene oxide with the effect of denitrifying bacteria to form graphene; after finishing the culturing, using hydrochloric acid, alcohol, and deionized water to remove bacterial cells, metabolite, and other ions produced in the reduction process, using water to cleaning until the water is neutral finally, and drying to obtain pure graphene. The method using microorganism denitrifying bacteria capable of reducing high valence state inorganic nitrogen and carbon as the microbial strain to conduct microbial reduction on graphene oxide; compared with similar reducing bacteria of Shewanella, the denitrifying bacteria has the advantages that the environmental safety is good, the reaction condition is mild and controllable, the medium component is simple, and the graphene obtained by preparation has less defects and layers.

Description

technical field [0001] The invention relates to a biologically reduced graphene oxide and a preparation method thereof, belonging to the field of biological materials. In particular, it concerns a microbe that reduces graphene oxide with a microbe, denitrifying bacteria. Background technique [0002] Because graphene has a series of excellent properties such as high specific surface area, outstanding thermal conductivity and mechanical properties, and its extraordinary electron transport performance, the preparation of high-quality and high-yield graphene has always been the focus of attention. Among them, the chemical oxidation-reduction method has become an effective way to realize large-scale preparation of graphene, that is, graphite is oxidized into graphite oxide, and then a dispersed graphene oxide solution is obtained under ultrasonic conditions, and then graphene is obtained by reduction with a chemical reducing agent. In the process of reducing graphene oxide, in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B31/04C01B32/184
Inventor 孙东平朱春林杨加志黄洋范孟孟魏静蒋国民张衡
Owner NANJING UNIV OF SCI & TECH
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