A nanomaterial for enriching and purifying pathogenic microorganisms and its preparation method

A technology of pathogenic microorganisms and nanomaterials, applied in the field of nanomaterials for the rapid enrichment and purification of pathogenic microorganisms and its preparation, can solve the problems of large background interference, high processing requirements, and reliance on labeling technology, and achieve large specific surface area and good biophase Capacitance, the effect of good biocompatibility

Active Publication Date: 2019-09-20
INST OF PLA FOR DISEASE CONTROL & PREVENTION
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  • Claims
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Problems solved by technology

[0005] Aiming at many problems in the current on-site detection of infectious disease pathogens, such as high requirements for sample pretreatment, large background interference, and dependence on labeling technology, the present invention intends to provide a solution that can be applied to samples in the pretreatment process of pathogenic microorganisms through the core-shell nanoparticle technology. Rapid enrichment and purification of multifunctional nanocomposites

Method used

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  • A nanomaterial for enriching and purifying pathogenic microorganisms and its preparation method
  • A nanomaterial for enriching and purifying pathogenic microorganisms and its preparation method
  • A nanomaterial for enriching and purifying pathogenic microorganisms and its preparation method

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Embodiment 1

[0027] Embodiment 1: preparation embodiment

[0028] (1) use Monodisperse silica nanospheres were prepared by this method (reference: W. , A.Fink.Controlled growth of monodisperse silica spheres in the micron sizerange[J].J.Colloid Interface Sci., 1968,26(1):62-69);

[0029] (2) Prepare ferric oxide-coated silica nanospheres by thermal deposition method, the specific preparation process is as follows: 0.25g Add the silica nanospheres prepared by the method into a mixed solution containing 50ml of ethylene glycol and 150ml of water. After the ultrasonic dispersion is uniform, the reaction solution is transferred to a three-necked flask, and mechanically stirred in a water bath at 45°C. After 15 minutes, 0.25 g of ferrous chloride was added to the reaction system, and mechanically stirred in a water bath at 45° C. for 5 hours. After the reaction was completed, the obtained black solution was centrifuged, and washed three times with deionized water and ethanol to obtain cor...

Embodiment 2

[0034] Embodiment 2 application embodiment

[0035] figure 2It is a schematic diagram of the rapid enrichment and purification of pathogenic microorganisms using nanomaterials prepared in the present invention. The figure shows that the nano-magnetic beads can specifically bind to the target antigen after being modified by a specific antibody, and the antigen-antibody immune complex obtained after enrichment and separation can be used for the next step of detection.

[0036] Take a 100ml small beaker, pour the pre-prepared simulated sample of known pathogenic microorganism concentration and the complex medium dilution into the beaker for use, and put the core-shell type multifunctional magnetic nanomaterial (hereinafter referred to as magnetic bead) prepared above Modify the antibody that can specifically recognize the target pathogenic microorganism, and then put the modified magnetic beads into the beaker and incubate with the sample to be tested at room temperature for 0....

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Abstract

The invention discloses a nanomaterial for enrichment and purification of pathogenic microorganisms and a preparation method of the nanomaterial, wherein the chemical structure of the nanomaterial is represented as SiO2@Fe3O4@SiO2@CdTe@CMCS. The nanomaterial is a core-shell multifunctional magnetic nanomaterial which is compatible with functions of targeting delivery, fluorescence labeling and specific binding, and is capable of rapidly and effectively enriching pathogenic microorganisms when diagnosing infectious diseases in the field.

Description

technical field [0001] The invention belongs to the field of nano biomedical materials, and relates to a nano material used for rapid enrichment and purification of pathogenic microorganisms and a preparation method thereof. Background technique [0002] At present, there are many kinds of biological detection methods, but the pre-treatment of samples is the key to determine the detection efficiency and identification results. Although the emergence and application of modern technologies such as biosensors have greatly promoted the rapid detection of infectious disease pathogens, they still cannot fully meet the needs of on-site rapid detection. The complex system where the pathogens of infectious diseases such as the respiratory tract and intestinal tract are located, such as exhaled breath, throat swabs and feces, etc., interference with the detection performance of sensors is a relatively common phenomenon, mainly manifested in: First, the presence of The content of non-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/34G01N1/40G01N33/569B82Y25/00B82Y40/00
Inventor 郝荣章宋宏彬李杨邱少富林睿王勇张传福吴志豪王立贵李鹏陈泽良李悦黄留玉
Owner INST OF PLA FOR DISEASE CONTROL & PREVENTION
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