Preparation method for antibacterial silk fibroin membrane by biological enzyme method

A biological enzyme method and antibacterial silk technology, which is applied in the preparation methods of peptides, chemical instruments and methods, and organic chemistry, etc., can solve the problems of low strength of ordinary silk fibroin membrane, hard and brittle membrane body, etc., and achieve good antibacterial effect, Improved antibacterial properties and significant performance

Active Publication Date: 2013-12-04
江苏紫霞生物科技有限公司
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The silk fibroin film prepared by this method has good mechanical and mechanical properties, which solves the defects of low strength, hard and brittle fi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of silk fibroin solution: add the degummed silkworm silk to the lithium bromide-ethanol-water (mass ratio 45:44:11) solution, and stir continuously at 70°C until dissolved (bath ratio 1:50), to obtain mixture. Put the silk fibroin solution into a dialysis bag, dialyze with deionized water for 24 hours, change the water every hour during this period, and filter to obtain silk fibroin aqueous solution after dialysis.

[0024] (2) Tyrosinase treatment: add tyrosinase (200 U / g silk fibroin) to the silk fibroin solution, and treat for 6 hours at a temperature of 30° C. and pH=7.0.

[0025] (3) Polypeptide antibacterial agent grafting: after tyrosinase treatment, add ε-polylysine (2g / L) in the enzyme bath for enzymatic grafting, and treat at a temperature of 30°C and pH=7.0 12 hours.

[0026] (4) Drying: After the reaction, 40 ml of silk fibroin solution was poured into a polystyrene watch glass, and dried at 50° C. for 6 hours to form a smooth and transpare...

Embodiment 2

[0031] (1) Preparation of silk fibroin solution: add the degummed silkworm silk to the calcium chloride-ethanol-water (molar ratio 1:2:8) solution, and stir continuously at 70°C until dissolved (bath ratio 1:50) , to obtain a mixed solution. Fill the silk fibroin solution into a dialysis bag, dialyze with deionized water for 24 hours, change the water every hour during this period, and filter after dialyzing to obtain silk fibroin aqueous solution.

[0032] (2) Tyrosinase and polypeptide antibacterial agent treatment: add tyrosinase (100U / g silk fibroin) and L-polyarginine (1g / L) simultaneously in the silk fibroin solution, at a temperature of 30°C, Treat in the same bath for 12 hours under the condition of pH=7.0.

[0033] (3) Drying: After the reaction, 40 ml of silk fibroin solution was poured into a polypropylene container, and dried at 50° C. for 6 hours to form an antibacterial silk fibroin film.

[0034] Sample 3: only add L-polyarginine treatment sample;

[0035] Sa...

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Abstract

The invention discloses a preparation method for an antibacterial silk fibroin membrane by a biological enzyme method, belongs to the field of textile biological technology, and aims to solve the disadvantages that a silk fibroin membrane prepared by a conventional method has a low strength and a hard and brittle membrane body, while avoiding the defects of large pollution and low biological safety of the silk fibroin membrane in a membrane preparing process by a chemical crosslink method. A catalytic oxidation function of tyrosinase is utilized, while achieving an intermolecular cross-linking of silk fibroin, a polypeptide antibacterial agent (such as epsilon-polylysine and L-polyarginine) having a primary amine structure is grafted onto the surface of the silk fibroin, and thus application performance of the silk fibroin membrane is improved; the specific technological process includes: preparing a silk fibroin solution, carrying out catalytic oxidation of the silk fibroin by tyrosinase, carrying out enzymatic grafting by the polypeptide antibacterial agent, drying and forming the membrane. The silk fibroin membrane prepared by the preparation method of the invention not only enables antibacterial performance to be improved, but also enables the strength, the elasticity and other mechanical properties of the silk fibroin membrane material to be improved. Compared with the silk fibroin membrane preparation method based on chemical crosslink, the tyrosinase method used for preparation of the silk fibroin membrane has the advantages of low energy consumption, high efficiency and less pollution, and is beneficial for environmental protection.

Description

technical field [0001] The invention relates to a method for preparing an antibacterial silk fibroin film by biological enzymatic method, which is a method for improving the mechanical properties of the silk fibroin film by using tyrosinase to catalyze oxidation, and at the same time endowing the silk fibroin film with antibacterial properties, belonging to the textile industry. field of biotechnology. Background technique [0002] Silk fibroin has good biocompatibility and environmental friendliness. The membrane material processed with silk fibroin solution is non-toxic, non-irritating and antigenic, and has good water and air permeability. It is a natural biological product with excellent performance. With drug slow-release carrier. The performance of silk fibroin film depends on the morphology and structure of silk fibroin macromolecules. Under normal conditions, the aggregate structure of silk fibroin film obtained after drying is mainly amorphous, the film body is har...

Claims

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Application Information

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IPC IPC(8): C08J5/18C08J3/24C08L89/00C08L77/02C07K1/14C12P21/00
Inventor 王平崔莉邱华张金宁袁久刚王强范雪荣
Owner 江苏紫霞生物科技有限公司
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