A method for improving the antioxidant capacity of plants
A high-temperature, compound technology, applied in botany equipment and methods, plant products, angiosperms/flowering plants, etc., can solve problems such as structural damage, cell death, etc., achieve improved antioxidant capacity, good application prospects, and improved antioxidant capacity and the effect of resilience
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Embodiment 1
[0037] Example 1 Cloning of Eupatorium adenophorum EaispF1 gene
[0038]The seeds of Eupatorium adenophorum were collected from Yunnan Province. Mix the flower soil: vermiculite in a ratio of 2:1, put it into a flower pot with a diameter of 25cm, put the flower pot with the culture soil into a large pot filled with tap water, and wait for the water to slowly seep into the culture soil. Sprinkle the seeds of Eupatorium adenophorum on the moistened cultivation soil, spread a layer of fine soil with a thickness of 2 mm on top of the seeds, and finally cover the flower pot with paper to prevent excessive evaporation of water, and wait for the eupatorium adenophorum to dry after 5-7 days. After the Eupatorium seedlings grew out, the paper on the pots was removed, and they were cultivated in the greenhouse for 8 weeks. Extract total RNA from seedlings, such as figure 2 shown.
[0039] The cDNA sequence of the EaispF1 gene of Eupatorium adenophorum was screened out by in situ hyb...
Embodiment 2
[0044] Example 2 EaispF1 Gene Sequence Analysis
[0045] The full-length cDNA of EaispF1 gene is 859bp, has a complete development reading frame, and encodes a protein consisting of 231 amino acid residues, such as Figure 5 , it is speculated that the theoretical isoelectric point of the protein is 8.67, and the molecular weight is 24.8kDa. According to the prediction of the ChloroP system, the 1-28 amino acids at the nitrogen end encode a signal peptide, which is located in the chloroplast. Comparing the predicted amino acid sequence of the EaispF1 gene with the amino acid sequences of other ispF genes, it is found that EaispF1 belongs to the MECDP synthetase family and has high homology with other IspFs, such as Steviarebaudiana's homology of 95%. , 87% homology of Arabidopsis thaliana, 82% homology of catharanthus roseus, 78% homology of ginkgo biloba, 73% homology of yew (Taxusxmedia), alfalfa (Medicagotruncatua) homology 72%.
Embodiment 3
[0046] Embodiment 3 EaispF1 gene expression characteristics
[0047] (1) Expression characteristics of EaispF1 gene in different tissues
[0048] Extract total RNA from leaves, stems and roots of Eupatorium adenophorum, and perform real-time fluorescent quantitative PCR with specific primers IR-PF and IR-PR of EaispF1 gene, and specific primers ZUB-F and ZUB-R of internal reference gene UBQ7, and analyze Tissue expression specificity of EaispF1 gene, as Figure 5 . EaispF1 gene was expressed in roots, stems and leaves, and the relative expression level in leaves was the highest, which was 6 times that of roots, followed by stems, whose expression level was 2 times that of roots.
[0049] (2) Expression characteristics of EaispF1 gene under different light conditions
[0050] EaispF1 gene expression is affected by different light, such as Figure 6 . When Eupatorium adenophorum was transferred from normal light conditions to dark conditions, the expression of EaispF1 gene ...
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