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Bacillus preparation for preventing and controlling pathogenic bacteria in soil environments as well as preparation method and application thereof

A technology of bacillus and bacillus pumilus, which is applied in the field of preparation and application of bacillus for the prevention and treatment of pathogenic bacteria in the soil environment, and can solve problems such as the irrelevance of antibiotic abuse, the pollution of soil and water by disinfectants, and the threat to the survival of animals and plants.

Active Publication Date: 2015-03-04
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to control the epidemic, people generally use a large number of chemical disinfectants for disinfection, but the disinfectants pollute the soil and water, affect the vegetation and microbial communities in the soil, and pose a threat to the survival of animals and plants in the water.
In addition, in order to prevent and control livestock and poultry infectious diseases, the harm caused by the extensive use of antibiotics to humans is even more unpredictable. It makes a large number of microorganisms resistant to drugs. Great relationship

Method used

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  • Bacillus preparation for preventing and controlling pathogenic bacteria in soil environments as well as preparation method and application thereof
  • Bacillus preparation for preventing and controlling pathogenic bacteria in soil environments as well as preparation method and application thereof
  • Bacillus preparation for preventing and controlling pathogenic bacteria in soil environments as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] A bacillus preparation for preventing and controlling pathogenic bacteria in the soil environment: it is prepared by mixing equal volumes of bacterial liquids of three kinds of bacteria, and the three kinds of bacteria are all isolated by the inventor from soil contaminated by pathogenic bacteria in Hubei area. Three kinds of bacteria are respectively a kind of Bacillus pumilus D31 (abbreviated as D31 in the present invention), a kind of Bacillus amyloliquefaciens J3-1 (abbreviated as J3-1 in the present invention) and a kind of Bacillus amyloliquefaciens D66 (abbreviated as J3-1 in the present invention) referred to as D66), and their separation methods are the same. Its separation process is:

[0060] A. Prepare bilayer plates:

[0061] 1) inoculate Escherichia coli (the pathogenic bacteria used in the present invention is Escherichia coli, this bacterium is isolated from the pig farm soil, utilizes the mouse to carry out the animal regression test and confirms that...

Embodiment 2

[0081] A kind of preparation method of the bacillus preparation of preventing and treating soil pathogenic bacteria, its steps are:

[0082] The microbial preparation of the present invention contains three kinds of bacillus, D31, D66 and J3-1. The inventor mixed equal volumes of bacterial liquids of D31, D66, and J3-1, and then added peat and mixed thoroughly to obtain a soil microbial preparation for pollution prevention and control of pathogenic bacteria such as pathogenic Escherichia coli in livestock and poultry breeding areas. The preparation method of microbial preparation is specifically as follows:

[0083] A. Pick a single colony, inoculate D66, D31 and J3-1 into a PA bottle containing 5ml LB liquid medium, shake at 37°C, 180rpm, for 12h;

[0084] B. Transfer 1% of the inoculum to a new 500ml Erlenmeyer flask containing 250ml of LB culture medium, 37°C, 180rpm shaking culture until more than 90% of the spores are formed; the culture time of strains D66 and J3-1 is 7...

Embodiment 3

[0089] Plate inhibition zone test

[0090] 1 Experimental method

[0091] 1) Pick a single colony, inoculate antagonistic bacteria D31, D66, and J3-1 into PA bottles containing 5ml of LB, shake and culture for 48 hours at 28°C and 180rpm;

[0092] 2) Add 5ml of LB solid medium to the plate, and place the Oxford cup after solidification;

[0093] 3) Add 5ml of LB solid medium to the plate to fix the Oxford cup;

[0094] 4) After solidification, add 200 μl of antagonistic bacteria solution to the Oxford cup, and culture at 28°C for 48 hours;

[0095] 5) Inoculate Escherichia coli into a PA bottle containing 5ml of LB liquid medium, shake at 37°C, 180rpm, for 12h;

[0096] 6) Take 50 μl of Escherichia coli culture in 250ml of LB soft agar solid medium (agar content is 0.5% (agar mass as a percentage of medium volume) cooled to 50-60°C, and mix well;

[0097] 7) Add 4 ml of LB soft agar solid medium mixed with Escherichia coli to the plate in step 4, culture it statically at 2...

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Abstract

The invention discloses a bacillus preparation for preventing and controlling pathogenic bacteria in soil environments as well as a preparation method and an application thereof. The bacillus preparation comprises three bacterial strains which are bacillus amyloliquefaciens D66, bacillus amyloliquefaciens J3-1 and short bacillus D31 and are separated out from soil environments. According to the preparation method, the fermentation liquid of the three bacterial strains is mixed in a volume ratio of 1:1:1, and grass carbon is used for adsorbing thalli, so that the microorganism preparation is prepared. Lots of animal pathogenic bacteria exist in livestock breeding soil environments at present. The animal pathogenic bacteria, represented by escherichia coli, can exist in soil for long term and result in symptoms such as diarrhea of livestock and poultry. The microorganism preparation disclosed by the invention is mainly used for preventing and controlling the animal pathogenic bacteria, namely the escherichia coli, in the livestock and poultry breeding environments, has the advantages of obvious effect and fast effect and can be used for improving the immune ability of livestock and poultry and inhibiting and antagonizing the growth and the propagation of the pathogenic bacteria in the environments, therefore, the microorganism preparation has wide application prospect.

Description

technical field [0001] The invention belongs to the field of soil environment management, and in particular relates to a bacillus preparation for preventing and controlling soil environmental pathogens, and also relates to a preparation method of a bacillus preparation for preventing and controlling soil environmental pathogens, and also relates to a bacillus preparation for preventing and controlling soil environmental pathogens They have obvious antagonism to pathogenic Escherichia coli that cause diseases such as chicken colibacillosis, piglet yellow scour, piglet pullorum and swine edema. Background technique [0002] In recent years, the outbreak of infectious diseases of livestock and poultry has become more and more frequent, which not only endangers the health of livestock and poultry, causes huge economic losses, but also threatens human health. Among them, colibacillosis caused by pathogenic Escherichia coli is the most common infectious disease in the livestock an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/14A01N63/00A01P1/00B09C1/10C12R1/07
Inventor 黄巧云陈雯莉姚佳瑜
Owner HUAZHONG AGRI UNIV