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Preparation method and application of marine organism active hemostasis dressing

A hemostatic dressing, marine biological technology, applied in medical science, absorbent pads, bandages, etc., to achieve the effects of small particle size, good biodegradability, accelerated growth and epithelial formation

Active Publication Date: 2013-12-25
山东贝诺医药生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing hemostatic materials are limited to simple hemostasis, but there are relatively few studies on anti-infection, promotion of healing, promotion of tissue repair, prevention of adhesions, and reduction of scars.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Weigh 18.1g of Span 80, 18.1g of Tween 80, and 2.0g of stearic acid into 500ml of liquid paraffin, and stir at 72°C to form an O (water) / (oil) W emulsion. Dissolve 0.8g of hydroxypropyltrimethylammonium chloride chitosan in 50ml of double distilled water, dissolve 0.8g of chitosan in 50ml of acetic acid solution with a volume fraction of 1%, and mix the two evenly. Dissolve 1.2g sodium alginate into 100ml double distilled water, add the chitosan salt solution to the sodium alginate solution and stir fully to form a stable colloidal solution, then add it to the paraffin emulsion, and stir rapidly for 25 minutes , stirring speed 800-1000 rpm. Then add 40ml of calcium chloride solution with a mass fraction of 1.6%, keep the temperature at 72°C, stir at a low speed for 50 minutes, and the stirring speed is 80-100 rpm. Pour the mixture into a sand core and vacuum filter it. Wash 3 times with 100ml of isopropanol, dehydrate with 30%, 50%, 70%, and 100% gradient alcohol resp...

Embodiment 2

[0028] Weigh 18.7g of Span 80, 18.7g of Tween 80, and 4.2g of stearic acid into 500ml of liquid paraffin, and stir at 75°C to form an O (water) / (oil) W emulsion. Take 1.0g of hydroxypropyltrimethylammonium chloride chitosan and dissolve it in 50ml of double distilled water, and dissolve 1.0g of chitosan in 50ml of acetic acid solution with a volume fraction of 1%, and mix the two evenly. Dissolve 1.5g of sodium alginate into 100ml of double-distilled water, add the chitosan salt solution to the sodium alginate solution and stir well to form a stable colloidal solution, then add it to the paraffin emulsion, and stir quickly for 30 minutes , stirring speed 800-1000 rpm. Then add 40ml of calcium chloride solution with a mass fraction of 2.0%, keep the temperature at 75°C, stir at a low speed for 55 minutes, and the stirring speed is 80-100 rpm. Pour the mixture into a sand core and vacuum filter it. Wash 3 times with 100ml of isopropanol, dehydrate with 30%, 50%, 70%, and 100% ...

Embodiment 3

[0030] Weigh 18.4g of Span 80, 18.4g of Tween 80, and 6.1g of stearic acid into 500ml of liquid paraffin, and stir at 78°C to form an O (water) / (oil) W emulsion. Dissolve 1.2g of hydroxypropyltrimethylammonium chloride chitosan into 50ml double distilled water, and dissolve 1.2g of chitosan into 50ml of acetic acid solution with a volume fraction of 1%, and mix the two evenly. Dissolve 1.8g of sodium alginate into 100ml of double-distilled water, add the chitosan salt solution to the sodium alginate solution and stir well to form a stable colloidal solution, then add it to the paraffin emulsion, and stir rapidly for 45 minutes , stirring speed 800-1000 rpm. Then add 40ml of calcium chloride solution with a mass fraction of 2.4%, keep the temperature at 80°C, stir at a low speed for 1h, and the stirring speed is 80-100 rpm. Pour the mixture into a sand core and vacuum filter it. Wash 3 times with 100ml of isopropanol, dehydrate with 30%, 50%, 70%, and 100% gradient alcohol re...

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PUM

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Abstract

The invention discloses a preparation method and application of a marine organism active hemostasis dressing. The preparation method comprises the steps of adding equal sodium alga acid solution while stirring a water solution comprising hydroxypropyl trimethyl ammonium chloride chitosan and / or an acetum of chitosan so as to obtain a liquor 1, taking an emulsion comprising paraffin as a liquor 2, mixing the liquor 1 and the liquor 2 according to the proportion of (2-3):5 and stirring uniformly so as to obtain a stable emulsification system, adding a cross-linking agent calcium chloride solution for cross-linking and solidifying the emulsification system, then washing, dewatering and drying so as to obtain the marine organism active hemostasis dressing. The materials of the prepared microballoon product have good biocompatibility and biodegradability as well as special effects of resisting bacterium, stopping bleeding, and promoting healing, when being contacted with a wound, the cross-linking and solidification product can rapidly absorb moisture in blood and concentrate blood platelets and blood coagulation factors into sticky gel, thus effectively sealing the bleeding wound and enhancing the hemostasis performance of the microballoon material, and therefore, the marine organism active hemostasis dressing is stable, efficient and environment-friendly.

Description

Technical field [0001] The invention relates to a hemostatic dressing, in particular to a preparation method and application of a marine bioactive hemostatic dressing. Background technique [0002] Chitosan is the second largest natural biopolymer material after cellulose. It is non-toxic, good biocompatibility, anti-inflammatory, anti-adhesion, antibacterial, promotes wound healing, moisture absorption and moisturizing, and is easy to degrade. With many excellent properties, it can be degraded by lysozyme into monosaccharides that can be used by the body, showing good biodegradability, and can play a role in blood coagulation through various channels. Quaternary ammonium chitosan is a kind of cationic surfactant, which is positively charged in water. It can be adsorbed on the surface of microorganisms to form micelles, and gradually penetrate into the lipid layer and protein of the cytoplasm, thereby changing the permeability of the cell membrane. It is the leakage of cell...

Claims

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Application Information

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IPC IPC(8): C08J9/26C08J3/24A61L15/28A61L15/44A61L15/42
Inventor 王春青武容华
Owner 山东贝诺医药生物科技有限公司
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