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Preparation method of vitamin C-loaded silk fibroin/hyaluronic acid composite nanofibers

A technology of composite nanofibers and silk fibroin, applied in cellulose/protein conjugated rayon, fiber processing, rayon manufacturing, etc., can solve the problem that natural proteins cannot simulate extracellular matrix components, and achieve strong application value , easy to control, simple preparation method

Active Publication Date: 2013-12-25
JIAXING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the natural extracellular matrix is ​​composed of proteins and polysaccharides with a network structure of nanofibers, and the addition of a single natural protein cannot mimic the composition of the extracellular matrix.

Method used

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  • Preparation method of vitamin C-loaded silk fibroin/hyaluronic acid composite nanofibers
  • Preparation method of vitamin C-loaded silk fibroin/hyaluronic acid composite nanofibers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of silk fibroin:

[0030] First add 100 grams of cocoons to 1 liter of 0.5w / v% Na 2 CO 3 Boiling in an aqueous solution for 30 minutes, repeating the treatment three times, fully washing with distilled water, and drying in a drying oven at 45° C. to obtain degummed silk fibers. with CaCl 2 :C 2 h 5 OH:H 2 A ternary solvent was prepared at a molar ratio of O=1:2:8, and the silk fiber was hydrolyzed in a water bath at 70°C for 1 hour at a constant temperature at a bath ratio of 1:10 to obtain a completely dissolved brown-yellow silk fiber hydrolysis solution. Put the hydrolysis solution into a dialysis bag, dialyze with distilled water for 72 hours, put the dialyzed silk fiber hydrolysis solution in -80°C for 12 hours, and then freeze-dry it at -58°C until dry to obtain white, loose and porous silk fibroin .

Embodiment 2

[0032] 4.29 grams of silk fibroin prepared in Example 1 were dissolved in 10 milliliters of deionized water (the density of deionized water was 1.0 g / cm 3 , the same below), stir until completely dissolved, and obtain a silk fibroin solution with a mass fraction of 30%; dissolve 0.42 g of hyaluronic acid in 10 ml of deionized water, stir until completely dissolved, and obtain a transparent silk fibroin solution with a mass fraction of 4%. Hyaluronic acid solution, mix 8 grams of silk fibroin solution with 2 grams of hyaluronic acid solution, so that the mass ratio of the two solutions is 8:2, then add 0.05 grams of vitamin C sodium phosphate in the mixed solution and stir evenly, then Carry out electrospinning, spinning condition is voltage 20 kilovolts, receiving distance 150 millimeters, spinning rate 0.5 milliliters / hour, obtains the composite nanofiber membrane that average fiber diameter is 512 ± 123 nanometers, composite nanofiber membrane photo is as follows figure 1 As...

Embodiment 3

[0034] Dissolve 4.29 grams of silk fibroin prepared in Example 1 in 10 milliliters of deionized water, stir until completely dissolved, and obtain a silk fibroin solution with a mass fraction of 30%; dissolve 0.42 grams of hyaluronic acid in 10 milliliters of deionized water, Stir until completely dissolved to obtain a hyaluronic acid solution with a mass fraction of 4%. Mix 8 grams of silk fibroin solution with 2 grams of hyaluronic acid solution so that the mass ratio of the two solutions is 8:2. Add 0.2 gram of vitamin C sodium phosphate and stir evenly, then carry out electrospinning, the spinning condition is voltage 20 kilovolts, receiving distance 150 millimeters, spinning speed 0.5 milliliters / hour, obtains the average fiber diameter and is 532 ± 104 nanometers Composite nanofiber membrane.

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Abstract

The invention provides a preparation method of vitamin C-loaded silk fibroin / hyaluronic acid composite nanofibers. The preparation method comprises following steps: silk fibroin is dissolved in deionized water, and is stirred at room temperature until silk fibroin is dissolved completely so as to obtain a silk fibroin solution with a mass concentration of 30 to 35%; hyaluronic acid is dissolved in deionized water, and is stirred at room temperature until hyaluronic acid is dissolved completely so as to obtain a hyaluronic acid solution with a mass concentration of 2 to 4%; the silk fibroin solution and the hyaluronic acid solution are mixed so as to obtain a silk fibroin / hyaluronic acid mixed solution; sodium L-ascorbyl-2-phosphate is dissolved in the silk fibroin / hyaluronic acid mixed solution, and the mixture is stirred uniformly so as to obtain a mixed electrospinning solution; and the mixed electrospinning solution is subjected to electrospinning so as to obtain the vitamin C-loaded silk fibroin / hyaluronic acid composite nanofibers. The vitamin C-loaded silk fibroin / hyaluronic acid composite nanofibers are capable of simulating the components of extracellular matrix in composition, and the preparation method is capable of endowing the vitamin C-loaded silk fibroin / hyaluronic acid composite nanofibers with excellent physiological properties.

Description

technical field [0001] The invention relates to the technical field of nanofiber preparation, in particular to a method for preparing silk fibroin / hyaluronic acid composite nanofibers loaded with vitamin C (vitamin C for short). Background technique [0002] Silk fibroin is a natural protein that is abundant in nature. It has excellent properties such as good biocompatibility, biodegradability, good air permeability, moisture permeability, and non-immunogenicity. It has been widely used in tissue in recent years. engineering field. The high porosity, high specific surface area, and good moisture and air permeability of silk fibroin nanofibers have become the preferred materials for skin tissue regeneration and wound healing. Min et al. found that human keratinocytes and fibroblasts had good adhesion and diffusion on silk fibroin nanofibers. In the study of wound healing, comparing the effects of silk fibroin nanofibers, microfibers and membranes on human oral epithelial ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F8/02D01F8/18D01F1/10D01D5/00
Inventor 张葵花
Owner JIAXING UNIV
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