Method used for preparing probiotic preparations by using vitamin B2 fermentation liquid waste
A technology of probiotic preparations and fermentation waste liquid, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of complex process, high energy consumption and high cost, shorten the process flow, improve nutritional value, The effect of saving production cost
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Embodiment 1
[0034] The utilization vitamin B of this embodiment 2 The method for preparing probiotic preparations from fermented waste liquid comprises the following steps:
[0035] 1) Pretreatment of fermentation waste liquid: to vitamin B 2 Sodium bicarbonate and calcium hydroxide are added to the fermentation waste liquid, the mass ratio of the sodium bicarbonate and calcium hydroxide is 3:1, and the pH value of the waste liquid is adjusted to 6.5;
[0036] 2) Probiotic inoculation and fermentation: Add probiotic strains to the waste liquid, the probiotic strains are compound probiotic strains of yeast, actinomycetes and filamentous bacteria, and the amount added is 0.5% of the total mass of the waste liquid %, control the temperature of the waste liquid to be 38°C, start fermentation, and stop the fermentation when the pH value of the fermentation liquid reaches 5;
[0037] 3) Collect bacteria or spores: Centrifuge the fermentation broth at a speed of 10000r / min to collect bacteria ...
Embodiment 2
[0041] The utilization vitamin B of this embodiment 2 The method for preparing probiotic preparations from fermented waste liquid comprises the following steps:
[0042] 1) Pretreatment of fermentation waste liquid: to vitamin B 2 Ammonia water is added to the fermentation waste liquid to adjust the pH value of the waste liquid to 5.5;
[0043] 2) Inoculation and fermentation of probiotics: Add probiotic strains to the waste liquid. The probiotic strains are compound probiotic strains of actinomycetes and filamentous bacteria. The amount added is 0.5% of the total mass of the waste liquid. The waste liquid temperature is 41 ℃, start fermentation, when the content of bacteria or spores in the fermentation liquid reaches 1×10 10 When CFU / g, stop the fermentation;
[0044] 3) Collect bacteria or spores: Centrifuge the fermentation broth at a speed of 10,000r / min to collect bacteria or spores; the centrifuged liquid is concentrated and returned to the fermentation waste liquid ...
Embodiment 3
[0047] The utilization vitamin B of this embodiment 2 The method for preparing probiotic preparations from fermented waste liquid comprises the following steps:
[0048] 1) Pretreatment of fermentation waste liquid: to vitamin B 2 Sodium bicarbonate and calcium hydroxide are added to the fermentation waste liquid, the mass ratio of the sodium bicarbonate and calcium hydroxide is 4:1, and the pH value of the waste liquid is adjusted to 7.2;
[0049]2) Inoculation and fermentation of probiotics: Add probiotic strains to the waste liquid, the probiotic strains are Lactobacillus and Bifidobacterium strains, the amount added is 0.5% of the total mass of the waste liquid, and the temperature of the waste liquid is controlled at 39 ℃, start fermentation, when the content of bacteria or spores in the fermentation liquid reaches 1×10 10 When CFU / g, stop the fermentation;
[0050] 3) Collect bacteria or spores: Centrifuge the fermentation broth at a speed of 10000r / min to collect bac...
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