Immunofluorescence diagnostic reagent and preparation thereof and application for clinical diagnosis of human urinary tract pathogen infection

A urinary system infection and urinary system technology, applied in the field of immunochemical reagents, can solve the problems of high requirements for instruments, long test time, complicated operation, etc., achieve strong specificity, short test time, and reduce the effect of antibiotic abuse

Active Publication Date: 2014-01-08
牛远杰 +5
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problems in the prior art, such as complex operation, high requirements for instruments, relatively long test time, high cost, etc., and provides an immunofluorescence diagnostic reagent for clinical diagnosis of human urinary system pathogen infection and its preparation and application method

Method used

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  • Immunofluorescence diagnostic reagent and preparation thereof and application for clinical diagnosis of human urinary tract pathogen infection
  • Immunofluorescence diagnostic reagent and preparation thereof and application for clinical diagnosis of human urinary tract pathogen infection
  • Immunofluorescence diagnostic reagent and preparation thereof and application for clinical diagnosis of human urinary tract pathogen infection

Examples

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Embodiment 1

[0026] Example 1: Preparation of immunofluorescence diagnostic reagents for Corynebacterium pseudogenitalium

[0027] 1) Bacterial strain isolation: Collect pathogenic bacteria samples from the urine of patients with urinary tract infection in the laboratory. The standard strains were obtained by streak culture on bacterial blood medium plate for 24 hours. It was preliminarily confirmed to be the pathogenic strain by the laboratory bacteria detector. (Original initial detection was E. coli)

[0028] 2) Strain identification: identification of morphological and physiological and biochemical indicators, the main measurement items include: cell morphology, spores, Gram staining, catalase, motility, M.R., urease, malonate utilization, carbon source utilization (xylose, Sucrose, mannitol, sorbitol, trehalose), glucose to produce acid and gas, etc. The measurement results are shown in Table 1:

[0029] Table 1 Morphological, physiological and biochemical characteristics of Cory...

Embodiment 2

[0080] Example 2: Preparation of Immunofluorescence Diagnostic Reagent for Staphylococcus aureus

[0081] 1) Bacterial strain isolation: Collect pathogenic bacteria samples from the urine of patients with urinary tract infection in the laboratory. The standard strains were obtained by streak culture on bacterial blood medium plate for 24 hours. It was preliminarily confirmed to be the pathogenic strain by the laboratory bacteria detector. (Staphylococcus aureus)

[0082] 2) Strain identification: identification of morphological and physiological and biochemical indicators, the main measurement items include: cell morphology, spores, Gram staining, catalase, motility, M.R., urease, malonate utilization, carbon source utilization (xylose, Sucrose, mannitol, sorbitol, trehalose), glucose to produce acid and gas, etc. The measurement results are shown in Table 2:

[0083] Table 2: Morphological, physiological and biochemical characteristics of Staphylococcus aureus strains ...

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Abstract

The invention discloses an immunofluorescence diagnostic reagent and preparation thereof and application for clinical diagnosis of human urinary tract pathogen infection, and belongs to immunochemical reagents with fluorescence labels. The method includes preparing monoclonal antibodies or polyclonal antibodies by pathogen strains of urinary tract infection, then performing dehydration and condensation on the antibodies and fluorescent quantum dots through amide, connecting as fluorescent quantum dot antibody complex, and directly using for detecting a carrier material coated with an unknown antigen, such as a patient specimen sample to be tested, or detecting antigen pre-coated on the carrier material. The method has the advantages that specificity is high and performances are stable during detecting urinary system diseases inflected by different pathogens; operation and usage are facilitated, detection is rapid and accurate, and detecting time is short; clinicians are guided to medicate accurately, abuse of antibiotics is reduced, cost for diagnosis detection and treatment are reduced for a patient, and accordingly fine economical benefits and social benefits are produced.

Description

technical field [0001] The present invention relates to immunochemical reagents with fluorescent labels. Specifically, it relates to an immunofluorescence diagnostic reagent for clinical diagnosis of human urinary system pathogenic bacteria infection and its preparation and application method. Background technique [0002] Urinary tract infection is caused by pathogens, including pathogenic bacteria, molds, fungi, viruses, pathogens, etc., and pathogenic bacteria infection is the main source of infection. Can cause pyelonephritis, cystitis, urethritis and other diseases in general. In recent years, with the wide application of environmental conditions, the use of immunosuppressants, hormones and interventional techniques and other medical methods, the incidence of urinary tract infection has been increasing. According to reports, the incidence of urinary tract infection is second only to respiratory tract infection. Pathogens often invade the urinary system due to the weak...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/532G01N33/577G01N21/64
CPCG01N21/6428G01N33/533G01N33/569G01N2800/348
Inventor 牛远杰陈家童蒋宁尚芝群喻其林刘越
Owner 牛远杰
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