Microbial flocculant as well as preparation method and application thereof

A technology of microbial flocculants and floc-producing bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unclear straw resources, high collection and transportation costs, and low degree of industrialization, and reach the source Wide range of effects with high flocculation performance

Inactive Publication Date: 2014-01-22
JIAXING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, with the increase of straw production, the improvement of rural energy structure and the application of various alternative raw materials, coupled with unclear straw resources, unclear utilization status, scattered distribution, large volume, high collection and trans

Method used

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  • Microbial flocculant as well as preparation method and application thereof
  • Microbial flocculant as well as preparation method and application thereof
  • Microbial flocculant as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1 Preparation of fermentation agent

[0043] Trichoderma koningii CGMCC3.2774 and Aspergillus niger CGMCC3.06194 were inoculated in sterilized straw bran medium, cultured at 28°C for 7 days, and the number of spores in the solid substrate exceeded 10 10 spores / g to obtain the fermentation inoculum.

[0044] The formula of the straw bran medium is: 700g of wheat straw powder, 300g of bran, 2500mL of water, natural pH; after mixing, sterilize at 126°C for 2 hours.

[0045] 2 Preparation of microbial flocculant

[0046] Take 10kg each of fresh beets and fresh rice straws, put them into a blender after pulverization by a pulverizer, add water while stirring until the water content of the substrate is about 60%, add concentrated nitric acid by spraying, when the pH of the substrate is 3-4 Stop adding concentrated nitric acid, and continue to add water until the water content of the matrix reaches 65%. Add 40 g of the above-mentioned fermentation agent, stir evenly, take o...

Embodiment 2

[0058] 1 Preparation of fermentation agent

[0059] Trichoderma viride (Trichoderma viride) CGMCC3.5455 and Aspergillus tubingensis (Aspergillus tubingensis) CGMCC3.06402 were respectively inoculated in sterilized straw bran medium, cultured at 28°C for 5 days, and the number of spores in the solid substrate exceeded 10 9 spores / g to obtain the fermentation inoculant.

[0060] The formula of the straw bran medium is: 700g of wheat straw powder, 300g of bran, 2500mL of water, natural pH; after mixing, sterilize at 126°C for 2 hours.

[0061] 2 Preparation of microbial flocculant

[0062] Take 10kg each of fresh beet and air-dried rice straw, put them into a mixer after pulverization by a pulverizer, add water while stirring until the water content of the substrate is about 60%, add concentrated nitric acid by spraying, when the pH of the substrate is 3-4 Stop adding concentrated nitric acid, and continue to add water until the water content of the substrate reaches 65%. Add ...

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Abstract

The invention discloses a microbial flocculant as well as a preparation method and application thereof. The preparation method comprises the following steps: inoculating flocculant-producing bacteria in a coarse fiber raw material to prepare a fermentation substrate with water content of 50-80 percent and pH (potential of Hydrogen) of 2-7; performing aerobic fermentation and anaerobic fermentation in sequence to obtain a fermentation product; leaching the fermentation product by using alkali liquor; filtering and drying leaching liquor to prepare the microbial flocculant. The fiber raw material is biologically converted by using the specific flocculant-producing bacteria, and meanwhile, a large amount of metabolites are produced during growth of the flocculant-producing bacteria, so that the obtained microbial flocculant has higher flocculation performance; the microbial flocculant is especially suitable for high-efficiency economic treatment on low-concentration heavy metal wastewater; the waste vegetables and crop straws are used as the raw materials which are low in cost, readily available and have wide sources, so that the recycling of the large amount of waste straws is realized; no wastewater is produced during production of the microbial flocculant, energy conservation and environmental friendliness are realized, the production process is simple, and the operation is convenient.

Description

technical field [0001] The invention relates to the technical field of heavy metal wastewater treatment, in particular to a microbial flocculant and its preparation method and application. Background technique [0002] Heavy metals are one of the main pollutants of wastewater and residues in electroplating, tanning, smelting and other industries. The heavy metals entering the environment cannot be biodegraded, and the long-term toxicity to organisms and the environment after enrichment through the food chain is huge; on the other hand, natural water bodies and heavy metals in the soil are difficult to remove, and the treatment cost is extremely high. [0003] Commonly used methods for removing heavy metals from aqueous solutions include chemical precipitation, electrolysis, ion flotation, electrodialysis and reverse osmosis, ferrite, adsorption, etc., such as literature (Peng Renyong, Cheng Baozhen, Fe / C micro-electrolysis -Research on the treatment of copper in electroplat...

Claims

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Application Information

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IPC IPC(8): C12P1/02C02F1/52C02F1/62C02F1/28C12R1/66C12R1/69C12R1/885C12R1/685C12R1/845C12R1/785C12R1/645
Inventor 陆筑凤李加友于建兴沈洁
Owner JIAXING UNIV
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