A detection method of visual biochip

Abstract

The invention discloses a detection method for a visible biological chip. The detection method comprises the steps of mixing an antigen or an antibody or an acceptor of an object to be detected with a colored conjugate or a colored protein in a PBS (phosphate buffering solution), dispensing a sample into a reaction region of a carrier film base, and closing an active group which is not combined with the dispensed sample on the carrier film base with fetal bovine serum; respectively adding a mixed standard solution containing detection targets with different concentration gradients into partial reaction holes of the biological chip, respectively adding a solution of the object to be detected into the residual reaction holes, continuing to add monoclonal antibodies and labeled goat anti mouse IgG which correspond to the detection targets into all the reaction holes in sequence to generate reaction in a water bath, and performing washing with washing liquid; continuing to add developing agents into all the reaction holes for developing reaction, wherein after the reaction is finished, the content of the object to be detected in the sample can be visually and qualitatively judged according to the degree of the color of a final point or the content of the detection targets in the solution of the object to be detected can be quantitatively calculated through an instrument.

Description

technical field [0001] The invention relates to a biochip detection method, in particular to a biochip detection method that is convenient for visually judging the quality of the chip and visually detecting multi-indicators in a sample to be tested. Background technique [0002] Existing biochips refer to microarrays of high-density DNA, antigens, antibodies, cells or tissues coated on immobilized carriers. Using the ability of biomacromolecules to recognize each other specifically, arrange them in an orderly manner on a solid-phase carrier substrate (well plate, membrane, glass slide, polymer carrier, etc.), and simultaneously react with the detection sample and labeled biomolecules Or hybridization, a large amount of useful biological information can be obtained through automatic reading equipment. At present, biochip detection methods mainly include fluorescence method, chemiluminescence method, catalytic colorimetric method and so on. Due to the high price of detection...

AI Technical Summary

Problems solved by technology

At the same time, before the traditional biochip is tested, the chip product is colorless, and it is impossible to intuitively judge the quality of the chip, such as whether there are leaks, etc.