A method for protein-induced preparation of chiral polyaniline
A chiral polyaniline and protein technology, which is applied in the fields of chemistry and biochemistry, can solve the problems of complex biological extraction steps, cumbersome operation, high price, etc., and achieves the effects of simple, mild and environmentally friendly experimental conditions, wide sources and low price.
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Embodiment 1
[0028] Weigh 0.0204 g of DBSA and dissolve it in 9.7 mL of pH 2.0 buffer solution; add 0.125 mmol of aniline monomer after stirring evenly; weigh 10 mg of bovine hemoglobin and dissolve it in 200 μl of distilled water and add it to the system; then add the concentration to 9.823 mol 100 μL / L of hydrogen peroxide solution was added to the reaction system in four times within one hour. After stirring the reaction for 8 hours, 10 ml of methanol was added for demulsification to terminate the reaction. The product was collected by centrifugation after precipitation, and dried to obtain a dark green powder with an apparent yield of 138.8%. Characterized by circular dichroism, a positive circular dichroism peak is generated at about 475 nm, which proves that the product is chiral polyaniline, and its molar ellipticity value is 1100 degree cm. 2 ·decimole -1 . Circular dichroism spectrum as attached figure 1 shown.
Embodiment 2
[0030] Weigh 0.0408 g of DBSA and dissolve it in 9.5 mL of pH 2.0 buffer solution; add 0.250 mmol of aniline monomer after stirring evenly; weigh 2 mg of bovine hemoglobin and 8 mg of bovine serum albumin with 200 μl of distilled water to dissolve and add to the system ; Then, 100 μL of hydrogen peroxide solution with a concentration of 9.823 mol / L was added to the reaction system 8 times within one hour. After stirring the reaction for 12 hours, 10 ml of methanol was added for demulsification to terminate the reaction. The product was collected by centrifugation after precipitation, and dried to obtain a dark green powder with an apparent yield of 124.3%. Characterized by circular dichroism, a positive circular dichroism peak is generated at about 475 nm, which proves that the product is chiral polyaniline, and its molar ellipticity value is 910 degree·cm 2 ·decimole -1 . Circular dichroism spectrum as attached figure 2 shown.
Embodiment 3
[0032] Weigh 0.0216 g of SDS and dissolve it in 9.7 mL of pH 1.0 buffer solution; add 0.1875 mmol of aniline monomer after stirring evenly; weigh 10 mg of bovine hemoglobin and dissolve it in 200 μl of distilled water and add it to the system; then add the concentration to 12.5 mmol 100 μL / L of ammonium persulfate solution was added to the reaction system in 5 times within one hour. After stirring the reaction for 18 hours, 10 ml of methanol was added to break the demulsification, and the reaction was terminated. The product was collected by centrifugation after precipitation, and dried to obtain a dark green powder with an apparent yield of 121.2%. Characterized by circular dichroism, a positive circular dichroism peak is generated at about 475nm, which proves that the product is chiral polyaniline, and its molar ellipticity value is 860degree·cm 2 ·decimole -1 . Circular dichroism spectrum as attached image 3 shown
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