Preparation method for curcumin long circulating liposomes

A long-circulation liposome and curcumin technology, which is applied in liposome delivery, medical preparations of non-active ingredients, pharmaceutical formulas, etc., can solve the problems of easy aggregation, poor stability, and unsatisfactory action time of ordinary liposomes and other problems, to achieve the effect of reducing plasma clearance, evading recognition, and reducing affinity

Inactive Publication Date: 2014-03-19
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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Problems solved by technology

However, as a drug carrier, the action time of common liposomes is still not ide

Method used

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  • Preparation method for curcumin long circulating liposomes
  • Preparation method for curcumin long circulating liposomes
  • Preparation method for curcumin long circulating liposomes

Examples

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[0016] Example 1:

[0017] 1. Apparatus and materials

[0018] 1.1. instrument

[0019] CP225D electronic balance (Sartorius, Germany); UV-1700 ultraviolet spectrophotometer (Shimadzu, Japan); Agilent 1200 high performance liquid chromatograph (Agilent, USA); HJ-6B multi-head magnetic heating mixer (Jintan City Gold Wei Experimental Instrument Factory); SCIENTZ-ⅡD ultrasonic cell crusher (Ningbo Xinzhi Biotechnology Co., Ltd.); pH meter (Mettler Toledo, Switzerland); Optima ultra-speed low-temperature centrifuge (Beckman, USA); Labconco freeze drying Machine (Labconco, USA), Marvern measurement particle size analyzer (Malvern Instruments Co., Ltd., UK), JEM-1200EX transmission electron microscope (JEOL, Japan).

[0020] Medicines and reagents

[0021] Curcumin (Chengdu Pusi Biotechnology Co., Ltd., content> 98%, batch number 12041502); curcumin reference substance (China Institute for Food and Drug Control, content 98.8%, batch number 110823-201004); soy lecithin for injection (PC...

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Abstract

Provided is a preparation method for curcumin long circulating liposomes. The method comprises steps: curcumin, soybean lecithin, cholesterol and polyethylene glycol- distearoyl phosphatidyl ethanolamine are weighed in a mass ratio of 1:30-50:3-5:1-3; the above raw materials are dissolved in ethanol as an organic phase; a phosphate buffer salt solution with a pH value of 6-7 is taken as an aqueous phase; under a rotating speed of 1000-1500 r/min, the organic phase is dropwisely added in the organic phase with thermal insulation at the temperature of 40-50 DEG C slowly and at a constant speed; after the dropwise add, stirring is continued for 2-3h, the organic solvent is volatilized, and a solution of the curcumin long circulating liposomes is obtained. The beneficial effects of the preparation method are mainly that, Cur-LCL is prepared through ethanol injection method, the technology is simple, the entrapment rate is high, the particle sizes are small, the release in vitro is slow, and the in vivo pharmacokinetics data display that the plasma clearance is decreased obviously, the half-life period is remarkably prolonged, and the curcumin long circulating liposomes have certain long circulation effects.

Description

technical field [0001] The invention relates to a preparation method of curcumin long-circulation liposome. Background technique [0002] Curcumin (Cur) is the main active ingredient of the traditional Chinese medicine turmeric, which belongs to polyphenolic compounds. Modern research has clearly proved that it has various pharmacological effects such as anti-tumor, anti-inflammation, and anti-oxidation, and has small toxic and side effects. It has clinical application prospects good. However, due to the poor water solubility of curcumin, it is easily oxidized, the metabolism is rapid in the body, and the defects of low bioavailability limit the popularization and development of curcumin. Liposomes, as a new drug delivery system, have the advantages of targeting, sustained release, reducing drug toxicity and improving drug stability. However, the action time of ordinary liposomes as a drug carrier is still not ideal, and the long-term storage is easy to aggregate and the s...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K31/12A61K47/34A61P35/00A61P29/00A61P39/06
Inventor 李范珠尤佳王国伟魏颖慧徐骏军郭曼曼
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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