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Industrial preparation method of high-protein grape seed dregs

A grape seed meal, high protein technology, applied in protein food processing, protein food ingredients, food science and other directions, can solve the problems of poor development of protein resources, low comprehensive utilization rate of grape seeds, limited processing technology, etc. Achieve the effect of alleviating high protein demand for feed, improving application value and low production cost

Inactive Publication Date: 2014-03-26
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the comprehensive utilization rate of grape seeds in my country's wine manufacturers is still relatively low, and many of them are directly thrown away, resulting in a great waste of resources.
Grape seeds are rich in unsaturated fatty acids, multiple vitamins and minerals, and rich in protein amino acids. Due to the limited processing technology at present, the protein resources in grape seeds cannot be well developed. The protein content is below 15%. Ordinary miscellaneous dregs or returned to the field as fertilizer, resulting in a great waste of resources

Method used

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  • Industrial preparation method of high-protein grape seed dregs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: The specific steps of the industrial production method of high-protein grape seed meal are as follows.

[0021] figure 1 As shown, the grape seeds are pulverized and separated from the kernels and shells by a grinder to obtain grape seed kernels. The grape seed kernels are softened and tempered with 55°C water for 45 minutes, and 7wt% of water is kept. Dried to a moisture content of 3wt%, sent to a degreasing extractor; using a non-polar solvent ethyl acetate to spray and extract in stages, the extraction temperature was 45 ° C, and the extraction time was 130 minutes to obtain a mixed oil; the degreased grape seeds were degreased at low temperature dissolve, remove the solvent, add a mixed aqueous solution of cellulase 80g / L, hemicellulase 65g / L and pectinase 60g / L according to the ratio of solid to liquid 1:2 (g / ml) for enzymolysis reaction, The reaction temperature is controlled at 30°C, and the reaction time is controlled at 1080min; after the enzymol...

Embodiment 2

[0022] Embodiment 2: The specific steps of the industrial production method of high-protein grape seed meal are as follows.

[0023] The grape seeds are crushed and separated from the kernels and shells by a pulverizer to obtain grape seed kernels. The grape seed kernels are softened and tempered with 58°C water for 50 minutes to keep 9wt% of moisture and enter the rolling mill for rolling, and then dried at 79°C until the moisture content is 3.5 wt%, and sent to the degreasing extractor; the non-polar solvent petroleum ether was sprayed and extracted in stages, the extraction temperature was 48°C, and the time was 130min to obtain a mixed oil; the degreased grape seeds were desolvated at low temperature, and the solvent was removed. Add a mixed aqueous solution of 90g / L cellulase, 60g / L hemicellulase and 90g / L pectinase according to the solid-liquid ratio of 1:3 (g / ml) for enzymolysis reaction, and the reaction temperature is controlled at 35°C. The reaction time is controlle...

Embodiment 3

[0024] Embodiment 3: The specific steps of the industrial production method of high-protein grape seed meal are as follows.

[0025] The grape seeds are crushed and separated from the kernel and shell by a pulverizer to obtain grape seed kernels. The grape seed kernels are softened and tempered with 57°C water for 45 minutes, and the moisture of 8wt% is kept. Then they are rolled into a rolling mill, and then dried at 60°C until the moisture content is 4wt%. , sent to the degreasing extractor; the non-polar solvent dichloromethane was used to spray and extract in stages, the extraction temperature was 48 ° C, and the extraction time was 160 min to obtain the mixed oil; Add a mixed aqueous solution of 100g / L cellulase, 90g / L hemicellulase and 80g / L pectinase at a liquid ratio of 1:3 to carry out the enzymolysis reaction. The reaction temperature is controlled at 32°C and the reaction time is controlled at 1250min; After the decomposition reaction, the material is filtered, and ...

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PUM

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Abstract

The invention discloses an industrial preparation method of high-protein grape seed dregs, which comprises the following steps: (1) breaking the grape seeds and separating shells and kernels to obtain grape seed kernels; (2) softening and tempering the grape seed kernels with water, rolling, drying and degreasing; (3) performing low-temperature desolventizing on the degreased grape seed kernels, and adding an aqueous solution containing cellulase, hemicellulase and pectinase for an enzymolysis reaction; (4) after the enzymolysis reaction, performing solid-liquid separation on the material; adding a sodium hydroxide solution into the solid for dissolution, and stirring for reaction; after the reaction, performing solid-liquid separation; adjusting the pH value of the separated liquid to 3.8-4.2 to separate out protein dissolved in water; performing solid-liquid separation again to obtain the concentrate of grape seed kernel protein; (5) drying the concentrate of grape seed kernel protein to obtain the high-protein grape seed dregs. The method disclosed by the invention is simple in process and low in production cost; the product has high protein content and rich nutrition, wherein the protein content is greater than 49.5wt%, the ash content is less than 4wt%, the fiber content is less than 5wt%, and the amino acid content is greater than 95wt%.

Description

technical field [0001] The invention relates to a deep processing method of grape seeds, in particular to an industrial production method of high-protein grape seed meal. Background technique [0002] my country's grape cultivation area is vast, and the output is increasing year by year. Among them, 80% of the grapes are used for wine making, about 13% are used for food, and about 7% are used for fruit juice and other grape products. main source. However, the comprehensive utilization rate of grape seeds in my country's wine manufacturers is still relatively low, and many of them are directly thrown away, resulting in a great waste of resources. Grape seeds are rich in unsaturated fatty acids, multiple vitamins and minerals, and rich in protein amino acids. Due to the limited processing technology at present, the protein resources in grape seeds cannot be well developed. The protein content is below 15%. Ordinary miscellaneous dregs or returning them to the field as fertili...

Claims

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Application Information

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IPC IPC(8): A23J1/00A23J3/34
Inventor 宋伟光陈运霞周冬丽王英袁瑞赵亚南
Owner CHENGUANG BIOTECH GRP CO LTD
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