Industrial preparation method of high-protein grape seed dregs

[0020] Embodiment 1: The specific steps of the industrialized preparation method of the high-protein grapeseed meal are as follows.

[0021] figure 1 As shown, the grape seeds are crushed and separated from the kernels and shells by a pulverizer to obtain grape seed kernels. The grape seed kernels are softened and tempered with water at 55°C for 45 minutes, and the water at 7wt% is kept in the rolling mill. Dry to a moisture content of 3wt%, and then send it to a degreasing extractor; use the non-polar solvent ethyl acetate for staged spray extraction, the extraction temperature is 45°C, and the extraction time is 130min to obtain mixed oil; the degreasing grape seed kernels are subjected to low-temperature degreasing. Dissolve, remove the solvent, and add a mixed aqueous solution of cellulase 80g/L, hemicellulase 65g/L and pectinase 60g/L to carry out enzymatic hydrolysis reaction according to the material-to-liquid ratio of 1:2 (g/ml). The reaction temperature was controlled at 30°C, and the reaction time was controlled at 1080min; after the enzymatic hydrolysis reaction was over, the material was filtered, the lower filter residue was added with 7mmol/L sodium hydroxide solution to dissolve, the temperature was controlled at 40°C for stirring reaction, and the reaction time was controlled at 60min; After the reaction is completed, the filtrate is filtered and separated, and the filtrate enters the blending tank, and the protein dissolved in water is separated out by adjusting the pH to 3.8, and is separated by a horizontal centrifuge to obtain a concentrate of grape seed protein; the concentrate is dried at low temperature to obtain the protein content Grapeseed meal with 49.8wt%, 2.8wt% ash, 4.6wt% fiber and 95.8wt% amino acid content.

[0022] Embodiment 2: The specific steps of the industrialized preparation method of the high-protein grapeseed meal are as follows.

[0023] Grape seeds are crushed and separated from the kernels and shells by a pulverizer to obtain grape seed kernels. The grape seed kernels are softened and tempered with water at 58°C for 50 minutes, and the moisture of 9wt% is kept in the rolling mill for rolling, and then dried at 79°C to a moisture content of 3.5. wt%, sent to the degreasing extractor; the non-polar solvent petroleum ether was used for staged spray extraction, the extraction temperature was 48 °C, and the time was 130 min to obtain mixed oil; According to the solid-liquid ratio of 1:3 (g/ml), a mixed aqueous solution of cellulase 90g/L, hemicellulase 60g/L and pectinase 90g/L was added to carry out enzymatic hydrolysis reaction, and the reaction temperature was controlled at 35°C, The reaction time was controlled at 1400min; after the enzymatic hydrolysis reaction was over, the material was filtered, the lower filter residue was added with 8.5mmol/L sodium hydroxide solution to dissolve, the temperature was controlled at 45°C for stirring reaction, and the reaction time was controlled at 80min; after the reaction was over, filtration and separation, The filtrate enters the blending tank, and the protein dissolved in water is precipitated by adjusting the pH to 3.9, and is separated by a horizontal centrifuge to obtain a concentrate of grape seed protein; the concentrate is dried at low temperature to obtain a protein content of 50.6wt%, ash content 3.7wt%, fiber 4.2wt%, amino acid 97.6wt% grapeseed meal.

[0024] Example 3: The specific steps of the industrialized preparation method of high-protein grapeseed meal are as follows.

[0025] Grape seeds are crushed and separated from the husks by a pulverizer to obtain grape seed kernels. The grape seed kernels are softened and tempered with water at 57°C for 45 minutes, and the moisture of 8wt% is kept in the rolling mill for rolling, and then dried at 60°C to a moisture content of 4wt%. , sent to the degreasing extractor; the non-polar solvent dichloromethane was used for staged spray extraction, the extraction temperature was 48 °C, and the time was 160 min to obtain mixed oil; the degreasing grape seed kernels were desolvated at low temperature, and the solvent was removed, and the The liquid ratio is 1:3 and a mixed aqueous solution of cellulase 100g/L, hemicellulase 90g/L and pectinase 80g/L is added to carry out enzymatic hydrolysis reaction, the reaction temperature is controlled at 32°C, and the reaction time is controlled at 1250min; After the solution reaction is completed, the material is filtered, the lower filter residue is added with 10 mmol/L sodium hydroxide solution to dissolve, the temperature is controlled at 42 ° C to carry out the stirring reaction, and the reaction time is controlled at 75 min; after the reaction is completed, the filtrate is filtered and separated, and the filtrate enters the mixing tank. When the pH reaches 4.1, the protein dissolved in water is precipitated, and separated by a horizontal centrifuge to obtain a concentrate of grape seed protein; the concentrate is dried at low temperature to obtain a protein content of 51wt%, ash content of 3.7wt%, fiber Grapeseed meal with amino acid content of 96.1wt%.