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Nano-hydroxyapatite/silk fibroin composite membrane material and preparation method thereof

A nano-hydroxyapatite and silk fibroin technology, applied in medical science, prosthesis, etc., can solve the problems of poor dispersion of hydroxyapatite, affecting biological activity and biocompatibility, difficult to control the shape and size, etc. To achieve the effect of controllable morphology and size, good physical and chemical properties and biological properties, and good practical value

Active Publication Date: 2014-03-26
苏州盛泽科技创业园发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above preparation methods can combine silk fibroin and hydroxyapatite, the dispersion of hydroxyapatite in the composite material is poor, and the shape and size are difficult to control, which directly affects its biological activity and biocompatibility.

Method used

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  • Nano-hydroxyapatite/silk fibroin composite membrane material and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0032] A nano-hydroxyapatite / silk fibroin composite membrane material and a preparation method thereof, comprising the steps of:

[0033] (1) Natural mulberry silk was degummed by boiling 0.05% sodium carbonate solution for 30 minutes, and obtained pure silk fibroin fiber after repeating 3 times; the silk fibroin fiber was dissolved in LiBr:C 2 h 5 OH:H 2 O=45:44:11 (mass ratio) Stir and dissolve in a water bath at a constant temperature of 70±2°C to obtain a regenerated silk fibroin solution; obtain a silk fibroin aqueous solution with a mass fraction of 2.0% after dialysis and filtration; take 10 mL of silk fibroin Aqueous solution, according to the mass ratio of silk fibroin and calcium chloride 1:0.1, add calcium chloride to the silk fibroin aqueous solution, magnetically stir for 3 hours, and form a film at room temperature to obtain calcium chloride / regenerated silk fibroin containing calcium chloride membrane;

[0034] (2) Will (NH 4 ) 2 HPO 4 Prepared into 0.1mol...

Embodiment 2

[0037] A nano-hydroxyapatite / silk fibroin composite membrane material and a preparation method thereof, comprising the steps of:

[0038] (1) Natural mulberry silk is degummed by boiling 0.05% sodium carbonate solution for 30 minutes, repeating 3 times to obtain pure silk fibroin fiber; dissolving the silk fibroin fiber in CaCl 2 : C 2 h 5 OH: H 2 O=1:2:8 (molar ratio) Stir and dissolve in a water bath at a constant temperature of 70±2°C to obtain a regenerated silk fibroin solution; after dialysis, filtration, and concentration, obtain a silk fibroin aqueous solution with a mass fraction of 5.0%; take 8mL Silk fibroin aqueous solution, according to silk fibroin and Ca(OC 2 h 5 ) 2 The mass ratio of 1:0.25, adding Ca(OC 2 h 5 ) 2 , magnetically stirred for 1h, and formed into a film at room temperature to obtain a Ca(OC 2 h 5 ) 2 Ca(OC 2 h 5 ) 2 / Regenerated silk fibroin film;

[0039] (2) put H 3 PO 4 Prepared into 0.05mol / L H 3 PO 4 aqueous solution and a...

Embodiment 3

[0041] A nano-hydroxyapatite / silk fibroin composite membrane material and a preparation method thereof, comprising the steps of:

[0042] (1) Natural mulberry silk is degummed by boiling with 0.5% sodium carbonate solution for 30 minutes, repeating 3 times to obtain pure silk fibroin fiber; dissolve the silk fibroin fiber in 9.3M lithium bromide solution in a water bath with a constant temperature of 70±2°C Stir and dissolve in the pot to obtain a regenerated silk fibroin solution; after dialysis and filtration, obtain a silk fibroin aqueous solution with a mass fraction of 3.0%; take 10mL of the silk fibroin aqueous solution, and press the mass ratio of silk fibroin to diammonium hydrogen phosphate 1:0.1 , adding diammonium hydrogen phosphate to the silk fibroin aqueous solution, magnetic stirring for 2 hours, and forming a film at room temperature to obtain a diammonium hydrogen phosphate / regenerated silk fibroin film containing diammonium hydrogen phosphate;

[0043] (2) Ca...

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Abstract

The invention discloses a nano-hydroxyapatite / silk fibroin composite membrane material and a preparation method thereof. The preparation method comprises the following steps: (a) preparing a silk fibroin membrane; and (b) preparing the nano-hydroxyapatite / silk fibroin composite membrane material, wherein the size of the nano-hydroxyapatite in the composite membrane material is 10-500nm. The invention develops a novel preparation method of the nano-hydroxyapatite / silk fibroin composite membrane material in simple preparation process, so that the problem that the mechanical properties of the nano-hydroxyapatite / silk fibroin composite membrane material are poor is solved. The composite membrane material prepared can be biodegradable and is harmless to a human body, so that the composite membrane material is an ideal biomedical material.

Description

technical field [0001] The invention relates to a composite membrane material and a preparation method thereof, in particular to a nano-hydroxyapatite / silk fibroin composite membrane material and a preparation method thereof. The prepared composite membrane material can be applied to repair and replacement of bone tissue engineering. Background technique [0002] The treatment and repair of bone injury and bone defect is one of the common clinical difficulties in orthopedics. The traditional treatment method is autologous or allogeneic bone grafting for repair. Autologous bone grafting is effective, but it has the disadvantages of limited supply and secondary surgery. In addition, allogeneic bone grafting has immunogenicity (immune rejection) and the risk of spreading infectious diseases . Using biomaterials for treatment can be arbitrarily shaped according to the bone repair or defect to achieve a perfect shape repair, and through in vitro cell culture and expansion, it ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46
Inventor 明津法张锋左保齐
Owner 苏州盛泽科技创业园发展有限公司
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